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A novel method for examining corneal endothelial cell morphology in infants

A novel method for examining corneal endothelial cell morphology in infants
A novel method for examining corneal endothelial cell morphology in infants
Previous studies suggest that central corneal endothelial cell density (ECD) decreases from 6,100 cells/mm2 in neonates to 3,100 cells/mm2 in 10-year-olds. Currently, there is very sparse data regarding ECD in young children as well as the trend in which ECD decreases during childhood. This is because young children were unable to comply with existing clinic-based specular microscopes. Hence, we developed a novel method of imaging young children intra-operatively to help establish age-specific normative data for ECD and hexagonality of cells (%HEX). Children were either imaged using our novel technique whilst under general anesthesia (GA) or awake in clinic using a child friendly technique. 58 children were recruited (mean age: 5.50; range: 0.44-10.36). Our cohort displayed a significant linear decrease in ECD with age (r= -0.56, P<0.001). No correlation was found between %HEX and age (r=-0.10, P=0.48). We show that using a modern specular microscope and a relatively simple technique, it is possible to collect images from young infants and children.
1091-8531
1-5
Rufai, S.R.
b8a329cd-17a2-4b8d-967e-3895cdb8a7be
Tan, N.Y.
a6122d05-01a7-4280-9573-5740ae9b789a
Barbara, R.
a1fca9b2-c99a-4749-b706-8237235eebc3
Hartrey, R.
0878a041-3049-4f11-869d-b79aaff8b14a
Self, J.E.
0f6efc58-ae24-4667-b8d6-6fafa849e389
Rufai, S.R.
b8a329cd-17a2-4b8d-967e-3895cdb8a7be
Tan, N.Y.
a6122d05-01a7-4280-9573-5740ae9b789a
Barbara, R.
a1fca9b2-c99a-4749-b706-8237235eebc3
Hartrey, R.
0878a041-3049-4f11-869d-b79aaff8b14a
Self, J.E.
0f6efc58-ae24-4667-b8d6-6fafa849e389

Rufai, S.R., Tan, N.Y., Barbara, R., Hartrey, R. and Self, J.E. (2017) A novel method for examining corneal endothelial cell morphology in infants. Journal of American Association for Pediatric Ophthalmology and Strabismus, 1-5. (doi:10.1016/j.jaapos.2017.01.002).

Record type: Article

Abstract

Previous studies suggest that central corneal endothelial cell density (ECD) decreases from 6,100 cells/mm2 in neonates to 3,100 cells/mm2 in 10-year-olds. Currently, there is very sparse data regarding ECD in young children as well as the trend in which ECD decreases during childhood. This is because young children were unable to comply with existing clinic-based specular microscopes. Hence, we developed a novel method of imaging young children intra-operatively to help establish age-specific normative data for ECD and hexagonality of cells (%HEX). Children were either imaged using our novel technique whilst under general anesthesia (GA) or awake in clinic using a child friendly technique. 58 children were recruited (mean age: 5.50; range: 0.44-10.36). Our cohort displayed a significant linear decrease in ECD with age (r= -0.56, P<0.001). No correlation was found between %HEX and age (r=-0.10, P=0.48). We show that using a modern specular microscope and a relatively simple technique, it is possible to collect images from young infants and children.

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More information

Submitted date: 17 November 2016
Accepted/In Press date: 13 January 2017
e-pub ahead of print date: 24 March 2017
Organisations: Clinical & Experimental Sciences

Identifiers

Local EPrints ID: 404224
URI: https://eprints.soton.ac.uk/id/eprint/404224
ISSN: 1091-8531
PURE UUID: 3b8ed47d-46e3-4962-82d8-42a387834357
ORCID for J.E. Self: ORCID iD orcid.org/0000-0002-1030-9963

Catalogue record

Date deposited: 18 Feb 2017 00:23
Last modified: 03 Dec 2019 06:18

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