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ETHYLENE RESPONSE FACTOR 74 (ERF74) plays an essential role in controlling a respiratory burst oxidase homolog D (RbohD)-dependent mechanism in response to different stresses in Arabidopsis

ETHYLENE RESPONSE FACTOR 74 (ERF74) plays an essential role in controlling a respiratory burst oxidase homolog D (RbohD)-dependent mechanism in response to different stresses in Arabidopsis
ETHYLENE RESPONSE FACTOR 74 (ERF74) plays an essential role in controlling a respiratory burst oxidase homolog D (RbohD)-dependent mechanism in response to different stresses in Arabidopsis
Recent studies indicate that the ETHYLENE RESPONSE FACTOR VII (ERF-VII) transcription factor is an important regulator of osmotic and hypoxic stress responses in plants. However, the molecular mechanism of ERF-VII-mediated transcriptional regulation remains unclear.

Here, we investigated the role of ERF74 (a member of the ERF-VII protein family) by examining the abiotic stress tolerance of an ERF74 overexpression line and a T-DNA insertion mutant using flow cytometry, transactivation and electrophoretic mobility shift assays.

35S::ERF74 showed enhanced tolerance to drought, high light, heat and aluminum stresses, whereas the T-DNA insertion mutant erf74 and the erf74;erf75 double mutant displayed higher sensitivity. Using flow cytometry analysis, we found that erf74 and erf74;erf75 lines lack the reactive oxygen species (ROS) burst in the early stages of various stresses, as a result of the lower expression level of RESPIRATORY BURST OXIDASE HOMOLOG D (RbohD). Furthermore, ERF74 directly binds to the promoter of RbohD and activates its expression under different abiotic stresses. Moreover, induction of stress marker genes and ROS-scavenging enzyme genes under various stress conditions is dependent on the ERF74?RbohD?ROS signal pathway.

We propose a pathway that involves ERF74 acting as an on–off switch controlling an RbohD-dependent mechanism in response to different stresses, subsequently maintaining hydrogen peroxide (H2O2) homeostasis in Arabidopsis.
0028-646X
1-15
Yao, Yuan
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He, Run Jun
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Xie, Qiao Li
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Zhao, Xian hai
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Deng, Xiao mei
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He, Jun bo
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Song, Lili
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He, Jun
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Marchant, Alan
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Chen, Xiao-Yang
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Wu, Ai-Min
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Yao, Yuan
04d74e15-8140-4330-9bf0-6a5602f653d2
He, Run Jun
207e9f21-bf43-44df-8b77-54c45e9d715e
Xie, Qiao Li
de0eca5f-a34d-46e1-884a-2ec663a14dcb
Zhao, Xian hai
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Deng, Xiao mei
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He, Jun bo
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Song, Lili
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He, Jun
d190c383-8093-4c9c-aade-c3a1fb3ae78f
Marchant, Alan
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Chen, Xiao-Yang
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Wu, Ai-Min
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Yao, Yuan, He, Run Jun, Xie, Qiao Li, Zhao, Xian hai, Deng, Xiao mei, He, Jun bo, Song, Lili, He, Jun, Marchant, Alan, Chen, Xiao-Yang and Wu, Ai-Min (2016) ETHYLENE RESPONSE FACTOR 74 (ERF74) plays an essential role in controlling a respiratory burst oxidase homolog D (RbohD)-dependent mechanism in response to different stresses in Arabidopsis. New Phytologist, 1-15. (doi:10.1111/nph.14278).

Record type: Article

Abstract

Recent studies indicate that the ETHYLENE RESPONSE FACTOR VII (ERF-VII) transcription factor is an important regulator of osmotic and hypoxic stress responses in plants. However, the molecular mechanism of ERF-VII-mediated transcriptional regulation remains unclear.

Here, we investigated the role of ERF74 (a member of the ERF-VII protein family) by examining the abiotic stress tolerance of an ERF74 overexpression line and a T-DNA insertion mutant using flow cytometry, transactivation and electrophoretic mobility shift assays.

35S::ERF74 showed enhanced tolerance to drought, high light, heat and aluminum stresses, whereas the T-DNA insertion mutant erf74 and the erf74;erf75 double mutant displayed higher sensitivity. Using flow cytometry analysis, we found that erf74 and erf74;erf75 lines lack the reactive oxygen species (ROS) burst in the early stages of various stresses, as a result of the lower expression level of RESPIRATORY BURST OXIDASE HOMOLOG D (RbohD). Furthermore, ERF74 directly binds to the promoter of RbohD and activates its expression under different abiotic stresses. Moreover, induction of stress marker genes and ROS-scavenging enzyme genes under various stress conditions is dependent on the ERF74?RbohD?ROS signal pathway.

We propose a pathway that involves ERF74 acting as an on–off switch controlling an RbohD-dependent mechanism in response to different stresses, subsequently maintaining hydrogen peroxide (H2O2) homeostasis in Arabidopsis.

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Accepted/In Press date: 15 September 2016
e-pub ahead of print date: 7 November 2016
Organisations: Centre for Biological Sciences

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Local EPrints ID: 404954
URI: http://eprints.soton.ac.uk/id/eprint/404954
ISSN: 0028-646X
PURE UUID: a2e55b9f-b333-4435-9ee3-b62c2392d4bc

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Date deposited: 25 Jan 2017 11:49
Last modified: 16 Dec 2019 19:30

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Contributors

Author: Yuan Yao
Author: Run Jun He
Author: Qiao Li Xie
Author: Xian hai Zhao
Author: Xiao mei Deng
Author: Jun bo He
Author: Lili Song
Author: Jun He
Author: Alan Marchant
Author: Xiao-Yang Chen
Author: Ai-Min Wu

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