Reduced fetal vitamin D status by maternal undernutrition during discrete gestational windows in sheep
Reduced fetal vitamin D status by maternal undernutrition during discrete gestational windows in sheep
Placental transport of vitamin D and other nutrients (e.g. amino acids, fats and glucose) to the fetus is sensitive to maternal and fetal nutritional cues. We studied the effect of maternal calorific restriction on fetal vitamin D status and the placental expression of genes for nutrient transport (aromatic T-type amino acid transporter-1 [TAT-1]; triglyceride hydrolase / lipoprotein uptake facilitator lipoprotein lipase [LPL]) and vitamin D homeostasis (CYP27B1; vitamin D receptor [VDR]), and their association with markers of fetal cardiovascular function and skeletal muscle growth. Pregnant sheep received 100% total metabolizable energy (ME) requirements (control), 40% total ME requirements peri-implantation (PI40, 1–31 days of gestation [dGA]) or 50% total ME requirements in late gestation (L, 104–127 dGA). Fetal, but not maternal, plasma 25-hydroxy-vitamin D (25OHD) concentration was lower in PI40 and L maternal undernutrition groups (p<0.01) compared with the control group at 0.86 gestation. PI40 group placental CYP27B1 mRNA levels were increased (p<0.05) compared with the control group. Across all groups, higher fetal plasma 25OHD concentration was associated with higher skeletal muscle myofibre and capillary density (p<0.05). In the placenta, higher VDR mRNA levels were associated with higher TAT-1 (p<0.05) and LPL (p<0.01) mRNA levels. In the PI40 maternal undernutrition group only, reduced fetal plasma 25OHD concentration may be mediated in part by altered placental CYP27B1. The association between placental mRNA levels of VDR and nutrient transport genes suggests a way in which the placenta may integrate nutritional cues in the face of maternal dietary challenges and alter fetal physiology.
vitamin D, undernutrition, fetus, placental, nutrient transport
370-381
Cleal, J. K.
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Hargreaves, M. R.
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Poore, K. R.
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Tang, J. C. Y.
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Fraser, W. D.
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Hanson, M. A.
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Green, L. R.
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June 2017
Cleal, J. K.
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Hargreaves, M. R.
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Poore, K. R.
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Tang, J. C. Y.
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Fraser, W. D.
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Hanson, M. A.
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Green, L. R.
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Cleal, J. K., Hargreaves, M. R., Poore, K. R., Tang, J. C. Y., Fraser, W. D., Hanson, M. A. and Green, L. R.
(2017)
Reduced fetal vitamin D status by maternal undernutrition during discrete gestational windows in sheep.
Journal of Developmental Origins of Health and Disease, 8 (3), .
(doi:10.1017/S2040174417000149).
Abstract
Placental transport of vitamin D and other nutrients (e.g. amino acids, fats and glucose) to the fetus is sensitive to maternal and fetal nutritional cues. We studied the effect of maternal calorific restriction on fetal vitamin D status and the placental expression of genes for nutrient transport (aromatic T-type amino acid transporter-1 [TAT-1]; triglyceride hydrolase / lipoprotein uptake facilitator lipoprotein lipase [LPL]) and vitamin D homeostasis (CYP27B1; vitamin D receptor [VDR]), and their association with markers of fetal cardiovascular function and skeletal muscle growth. Pregnant sheep received 100% total metabolizable energy (ME) requirements (control), 40% total ME requirements peri-implantation (PI40, 1–31 days of gestation [dGA]) or 50% total ME requirements in late gestation (L, 104–127 dGA). Fetal, but not maternal, plasma 25-hydroxy-vitamin D (25OHD) concentration was lower in PI40 and L maternal undernutrition groups (p<0.01) compared with the control group at 0.86 gestation. PI40 group placental CYP27B1 mRNA levels were increased (p<0.05) compared with the control group. Across all groups, higher fetal plasma 25OHD concentration was associated with higher skeletal muscle myofibre and capillary density (p<0.05). In the placenta, higher VDR mRNA levels were associated with higher TAT-1 (p<0.05) and LPL (p<0.01) mRNA levels. In the PI40 maternal undernutrition group only, reduced fetal plasma 25OHD concentration may be mediated in part by altered placental CYP27B1. The association between placental mRNA levels of VDR and nutrient transport genes suggests a way in which the placenta may integrate nutritional cues in the face of maternal dietary challenges and alter fetal physiology.
Text
Cleal text clean DOHaD-11-16-OA-0735
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More information
Accepted/In Press date: 13 February 2017
e-pub ahead of print date: 22 March 2017
Published date: June 2017
Keywords:
vitamin D, undernutrition, fetus, placental, nutrient transport
Organisations:
Human Development & Health
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Local EPrints ID: 406441
URI: http://eprints.soton.ac.uk/id/eprint/406441
PURE UUID: f360373b-65fd-44bc-a521-69f29f7a0049
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Date deposited: 10 Mar 2017 10:47
Last modified: 16 Mar 2024 05:02
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Contributors
Author:
M. R. Hargreaves
Author:
J. C. Y. Tang
Author:
W. D. Fraser
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