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Integrated optical waveguide-based fluorescent immunosensor for fast and sensitive detection of microcystin-LR in lakes: optimization and analysis

Integrated optical waveguide-based fluorescent immunosensor for fast and sensitive detection of microcystin-LR in lakes: optimization and analysis
Integrated optical waveguide-based fluorescent immunosensor for fast and sensitive detection of microcystin-LR in lakes: optimization and analysis
Nowadays, biosensor technologies which can detect various contaminants in water quickly and cost-effectively are in great demand.Herein, we report an integrated channel waveguide-based fluorescent immunosensor with the ability to detect a maximum of 32 contaminants rapidly and simultaneously. In particular, we use waveguide tapers to improve the efficiency of excitation and collection of fluorescent signals in the presence of fluorophore photobleaching in a solid surface bioassay. Under the optimized waveguide geometry, this is the first demonstration of using such a type of waveguide immunosensor for the detection of microcystin-LR (MC-LR) in lake water. The waveguide chip was
22 activated by (3-Mercaptopropyl)23 trimethoxysilane/N-(4-maleimidobutyryloxy) succinimide (MTS/GMBS) for immobilization of BSA-MC-LR conjugate, which was confirmed to have uniform monolayer distribution by atomic force microscopy. All real lake samples, even those containing MC-LR in the sub-microgram per liter range (e.g. 0.5 μg/L), could be determined by the immunosensor with recovery rates between 84% and 108%, confirming its application potential in the measurement of MC-LR in real water samples.
2045-2322
Liu, Lanhua
2b7fba18-6358-48db-84c9-ccb526cf99db
Zhou, Xiaohong
bb88cffe-e54d-4ff8-827a-1d549f8ab376
Wilkinson, James S.
73483cf3-d9f2-4688-9b09-1c84257884ca
Hua, Ping
92fa76e2-970b-45f5-a459-d9f95e735303
Song, Baodong
23bb4743-8000-47f7-9094-f4faeecdc64b
Shi, Hanchang
c9b0c354-5f2a-40ac-991c-d334840819a3
Liu, Lanhua
2b7fba18-6358-48db-84c9-ccb526cf99db
Zhou, Xiaohong
bb88cffe-e54d-4ff8-827a-1d549f8ab376
Wilkinson, James S.
73483cf3-d9f2-4688-9b09-1c84257884ca
Hua, Ping
92fa76e2-970b-45f5-a459-d9f95e735303
Song, Baodong
23bb4743-8000-47f7-9094-f4faeecdc64b
Shi, Hanchang
c9b0c354-5f2a-40ac-991c-d334840819a3

Liu, Lanhua, Zhou, Xiaohong, Wilkinson, James S., Hua, Ping, Song, Baodong and Shi, Hanchang (2017) Integrated optical waveguide-based fluorescent immunosensor for fast and sensitive detection of microcystin-LR in lakes: optimization and analysis. Scientific Reports, 7 (1), [3655]. (doi:10.1038/s41598-017-03939-8).

Record type: Article

Abstract

Nowadays, biosensor technologies which can detect various contaminants in water quickly and cost-effectively are in great demand.Herein, we report an integrated channel waveguide-based fluorescent immunosensor with the ability to detect a maximum of 32 contaminants rapidly and simultaneously. In particular, we use waveguide tapers to improve the efficiency of excitation and collection of fluorescent signals in the presence of fluorophore photobleaching in a solid surface bioassay. Under the optimized waveguide geometry, this is the first demonstration of using such a type of waveguide immunosensor for the detection of microcystin-LR (MC-LR) in lake water. The waveguide chip was
22 activated by (3-Mercaptopropyl)23 trimethoxysilane/N-(4-maleimidobutyryloxy) succinimide (MTS/GMBS) for immobilization of BSA-MC-LR conjugate, which was confirmed to have uniform monolayer distribution by atomic force microscopy. All real lake samples, even those containing MC-LR in the sub-microgram per liter range (e.g. 0.5 μg/L), could be determined by the immunosensor with recovery rates between 84% and 108%, confirming its application potential in the measurement of MC-LR in real water samples.

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JSW-PH-SR-2017 - Accepted Manuscript
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s41598-017-03939-8 - Version of Record
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More information

Accepted/In Press date: 5 May 2017
e-pub ahead of print date: 16 June 2017
Published date: 1 December 2017
Organisations: Optoelectronics Research Centre, Photonic Systems Circuits & Sensors, Faculty of Physical Sciences and Engineering

Identifiers

Local EPrints ID: 410620
URI: http://eprints.soton.ac.uk/id/eprint/410620
ISSN: 2045-2322
PURE UUID: db46a990-bf82-4bb9-98da-8eb8ac1fdadf
ORCID for James S. Wilkinson: ORCID iD orcid.org/0000-0003-4712-1697

Catalogue record

Date deposited: 09 Jun 2017 09:15
Last modified: 18 Feb 2021 16:32

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