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Parylene C topographic micropattern as a template for patterning PDMS and Polyacrylamide hydrogel

Parylene C topographic micropattern as a template for patterning PDMS and Polyacrylamide hydrogel
Parylene C topographic micropattern as a template for patterning PDMS and Polyacrylamide hydrogel
Parylene C is a well-known polymer and it has been mainly employed as a protective layer for implantable electronics. In this paper, we propose a new approach to use Parylene C as a versatile template for patterning soft materials potentially applicable as scaffolds in cardiac tissue engineering (TE). Parylene C substrates were anisotropically patterned through standard lithographic process with hydrophilic channels separating raised hydrophobic strips. Ridges and grooves of the template are 10 µm width and depth ranging from 1 to 17 µm. Polydimethylsiloxane (PDMS) and Polyacrylamide (PAm) hydrogel have been chosen as soft polymers to be moulded. Thanks to their chemical and physical properties PDMS and PAm hydrogel mimic the extracellular matrix (ECM). PDMS was spin coated on micropatterned Parylene C obtaining composite substrates with 460 nm and 1.15 µm high grooves. The Young’s modulus of the composite Parylene C/PDMS was evaluated and it was found to be almost halfwhen compared to PDMS. PAm hydrogel was also printed using collagen coated micro-grooved Parylene C. Optical micrographs and fluorescence analysis show the successful topographic and protein pattern transfer on the hydrogel.
2045-2322
1-11
Sanzari, Ilaria
0044ac56-579f-4666-8ae1-47839b9742bf
Prodromakis, Themistoklis
d58c9c10-9d25-4d22-b155-06c8437acfbf
Sanzari, Ilaria
0044ac56-579f-4666-8ae1-47839b9742bf
Prodromakis, Themistoklis
d58c9c10-9d25-4d22-b155-06c8437acfbf

Sanzari, Ilaria and Prodromakis, Themistoklis (2017) Parylene C topographic micropattern as a template for patterning PDMS and Polyacrylamide hydrogel. Scientific Reports, 7, 1-11. (doi:10.1038/s41598-017-05434-6).

Record type: Article

Abstract

Parylene C is a well-known polymer and it has been mainly employed as a protective layer for implantable electronics. In this paper, we propose a new approach to use Parylene C as a versatile template for patterning soft materials potentially applicable as scaffolds in cardiac tissue engineering (TE). Parylene C substrates were anisotropically patterned through standard lithographic process with hydrophilic channels separating raised hydrophobic strips. Ridges and grooves of the template are 10 µm width and depth ranging from 1 to 17 µm. Polydimethylsiloxane (PDMS) and Polyacrylamide (PAm) hydrogel have been chosen as soft polymers to be moulded. Thanks to their chemical and physical properties PDMS and PAm hydrogel mimic the extracellular matrix (ECM). PDMS was spin coated on micropatterned Parylene C obtaining composite substrates with 460 nm and 1.15 µm high grooves. The Young’s modulus of the composite Parylene C/PDMS was evaluated and it was found to be almost halfwhen compared to PDMS. PAm hydrogel was also printed using collagen coated micro-grooved Parylene C. Optical micrographs and fluorescence analysis show the successful topographic and protein pattern transfer on the hydrogel.

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Parylene C topographic micropattern as a template for patterning PDMS and Polyacrylamide hydrogel. - Accepted Manuscript
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s41598-017-05434-6 - Version of Record
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More information

Accepted/In Press date: 16 June 2017
e-pub ahead of print date: 18 July 2017
Organisations: Nanoelectronics and Nanotechnology, Electronics & Computer Science

Identifiers

Local EPrints ID: 411312
URI: https://eprints.soton.ac.uk/id/eprint/411312
ISSN: 2045-2322
PURE UUID: fa2d14ba-7a3a-41f3-85df-bf3cd7897f3e
ORCID for Themistoklis Prodromakis: ORCID iD orcid.org/0000-0002-6267-6909

Catalogue record

Date deposited: 19 Jun 2017 16:30
Last modified: 03 Dec 2019 06:02

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