Preparation and characterization of large unilamellar vesicles mixed with trimethylchitosan (TMC): the effect of polyelectrolyte concentration
Preparation and characterization of large unilamellar vesicles mixed with trimethylchitosan (TMC): the effect of polyelectrolyte concentration
Background: the effect of different concentrations of the absorption enhancer trimethylchitosan (TMC) to the physicochemical properties of large unilamellar vesicles (LUV) comprised of L-a- Phospahtidylcholine (PC) were investigated in the current study.
Methods: the degree of quartenization (DQ) of trimethylchitosan was assessed with nuclear magnetic resonance (1H NMR). The vesicles were characterized by means of dynamic light scattering (DLS), ζ- potential, differential scanning calorimetry (DSC) and contact angle goniometry (CAG) measurements.
Results: the data showed that the surface charge of the PC liposomes was significantly altered as a function of the TMC concentration, giving evidence of presence of the polyelectrolyte to the liposome’s membrane. Varying the concentration of TMC affected the phase transition temperature (Tm) of the lipid, verifying the miscibility of the polyelectrolyte with the lipid bilayer. The association of the polymer with the liposomes was related to the amount of the polyelectrolyte present, reflecting changes to the wettability of the dispersion as measured by CAG.
Conclusions: the results demonstrated that presence of TMC significantly modified the physical properties of liposomes. Such systems might have a potential use for mucosal delivery (e.g. nasal route of administration).
van der Merwe, S.M.
d4864e20-f9f4-4be0-a06c-2db971432c96
Bouropoulos, N.
fadadea1-2aa2-4cd9-a7e5-33ba790cd45f
Lamprou, D.A.
f511a9e8-a769-462d-aee8-0fa1167f6c92
Katsamenis, O.L.
8553e7c3-d860-4b7a-a883-abf6c0c4b438
Fatouros, D.G.
4a716c96-a8ba-4fbf-b3db-3b2a3a2794c4
2017
van der Merwe, S.M.
d4864e20-f9f4-4be0-a06c-2db971432c96
Bouropoulos, N.
fadadea1-2aa2-4cd9-a7e5-33ba790cd45f
Lamprou, D.A.
f511a9e8-a769-462d-aee8-0fa1167f6c92
Katsamenis, O.L.
8553e7c3-d860-4b7a-a883-abf6c0c4b438
Fatouros, D.G.
4a716c96-a8ba-4fbf-b3db-3b2a3a2794c4
van der Merwe, S.M., Bouropoulos, N., Lamprou, D.A., Katsamenis, O.L. and Fatouros, D.G.
(2017)
Preparation and characterization of large unilamellar vesicles mixed with trimethylchitosan (TMC): the effect of polyelectrolyte concentration.
Current Bionanotechnology, 3 (1).
(doi:10.2174/2213529403666170126163928).
Abstract
Background: the effect of different concentrations of the absorption enhancer trimethylchitosan (TMC) to the physicochemical properties of large unilamellar vesicles (LUV) comprised of L-a- Phospahtidylcholine (PC) were investigated in the current study.
Methods: the degree of quartenization (DQ) of trimethylchitosan was assessed with nuclear magnetic resonance (1H NMR). The vesicles were characterized by means of dynamic light scattering (DLS), ζ- potential, differential scanning calorimetry (DSC) and contact angle goniometry (CAG) measurements.
Results: the data showed that the surface charge of the PC liposomes was significantly altered as a function of the TMC concentration, giving evidence of presence of the polyelectrolyte to the liposome’s membrane. Varying the concentration of TMC affected the phase transition temperature (Tm) of the lipid, verifying the miscibility of the polyelectrolyte with the lipid bilayer. The association of the polymer with the liposomes was related to the amount of the polyelectrolyte present, reflecting changes to the wettability of the dispersion as measured by CAG.
Conclusions: the results demonstrated that presence of TMC significantly modified the physical properties of liposomes. Such systems might have a potential use for mucosal delivery (e.g. nasal route of administration).
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Accepted/In Press date: 17 January 2017
Published date: 2017
Organisations:
Engineering Mats & Surface Engineerg Gp
Identifiers
Local EPrints ID: 411648
URI: http://eprints.soton.ac.uk/id/eprint/411648
ISSN: 2213-5294
PURE UUID: 7f29046b-66dc-4dba-a42b-dbef17d7c1d6
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Date deposited: 21 Jun 2017 16:32
Last modified: 16 Mar 2024 05:14
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Author:
S.M. van der Merwe
Author:
N. Bouropoulos
Author:
D.A. Lamprou
Author:
D.G. Fatouros
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