Zeller-Plumhoff, Berit, Daly, Keith, Clough, Geraldine, Schneider, Philipp and Roose, Tiina (2017) Investigation of microvascular morphological measures for skeletal muscle tissue oxygenation by image-based modelling in 3D. Journal of the Royal Society Interface, 14 (135), [20170635]. (doi:10.1098/rsif.2017.0635).
Abstract
The supply of oxygen in sufficient quantity is vital for the correct functioning of all organs in the human body, namely for skeletal muscle during exercise. Traditionally, microvascular oxygen supply capability is assessed by analysis of morphological measures on transverse cross-sections of muscle, e.g., capillary density or capillary-to-fibre ratio. In this work we are investigating the relationship between microvascular structure and muscle tissue oxygenation in mice. Phase contrast imaging was performed using synchrotron radiation computed tomography (SR CT) to visualize red blood cells (RBCs) within the microvasculature in mouse soleus muscle. Image-based mathematical modelling of the oxygen diffusion from the red blood cells into the muscle tissue was subsequently performed, as well as a morphometric analysis of the microvasculature. The mean tissue oxygenation was then compared to morphological measures of the microvasculature. RBC volume fraction and spacing (mean distance of any point in tissue to closest RBC) emerged as the best predictors for muscle tissue oxygenation, followed by length density (summed RBC length over muscle volume). The 2D measures of capillary density and capillary-to-fibre ratio ranked last. We therefore conclude, that in order to assess states of health of muscle tissue it is advisable to rely on 3D morphological measures rather than the traditional 2D measures.
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- Faculties (pre 2018 reorg) > Faculty of Medicine (pre 2018 reorg) > Human Development & Health (pre 2018 reorg)
Current Faculties > Faculty of Medicine > Human Development and Health > Human Development & Health (pre 2018 reorg)
Human Development and Health > Human Development & Health (pre 2018 reorg) - Faculties (pre 2018 reorg) > Faculty of Engineering and the Environment (pre 2018 reorg) > Southampton Marine & Maritime Institute (pre 2018 reorg)
- Faculties (pre 2018 reorg) > Faculty of Engineering and the Environment (pre 2018 reorg) > Mechanical Engineering (pre 2018 reorg) > Bioengineering Group (pre 2018 reorg)
Current Faculties > Faculty of Engineering and Physical Sciences > School of Engineering > Mechanical Engineering > Mechanical Engineering (pre 2018 reorg) > Bioengineering Group (pre 2018 reorg)
Mechanical Engineering > Mechanical Engineering (pre 2018 reorg) > Bioengineering Group (pre 2018 reorg)
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