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A Lab-on-Chip Analyzer for in situ Measurement of Soluble Reactive Phosphate: Improved phosphate blue assay and application to fluvial monitoring

A Lab-on-Chip Analyzer for in situ Measurement of Soluble Reactive Phosphate: Improved phosphate blue assay and application to fluvial monitoring
A Lab-on-Chip Analyzer for in situ Measurement of Soluble Reactive Phosphate: Improved phosphate blue assay and application to fluvial monitoring
Here, we present a new in situ microfluidic phosphate sensor that features an improved “phosphate blue” assay which includes polyvinylpyrrolidone in place of traditional surfactants—improving sensitivity and reducing temperature effects. The sensor features greater power economy and analytical performance relative to commercially available alternatives, with a mean power consumption of 1.8 W, a detection limit of 40 nM, a dynamic range of 0.14–10 μM, and an infield accuracy of 4 ± 4.5%. During field testing, the sensor was continuously deployed for 9 weeks in a chalk stream, revealing complex relations between flow rates and phosphate concentration that suggest changing dominance in phosphate sources. A distinct diel phosphorus signal was observed under low flow conditions, highlighting the ability of the sensor to decouple geochemical and biotic effects on phosphate dynamics in fluvial environments. This paper highlights the importance of high resolution in situ sensors in addressing the current gross under-sampling of aquatic environments.
0013-936X
9989–9995
Clinton-Bailey, Geraldine S.
b1c0cbd2-32a3-4280-b097-28b596e3c117
Grand, Maxime
659acbde-d639-42b7-9fab-52fa1b3655ff
Beaton, Alexander D.
02f38dc0-7db0-488c-af92-465275f9d584
Nightingale, Adrian
4b51311d-c6c3-40d5-a13f-ab8917031ab3
Owsianka, David
429615d0-69a0-4db0-a241-2e6854f24f7f
Slavik, Gregory J.
3244a5e0-ac7a-4f25-b203-4e9b00a68478
Connelly, Douglas
d49131bb-af38-4768-9953-7ae0b43e33c8
Cardwell, Christopher L.
9e61f59c-959d-4dac-8320-8163f2a2158a
Mowlem, Matthew
6f633ca2-298f-48ee-a025-ce52dd62124f
Clinton-Bailey, Geraldine S.
b1c0cbd2-32a3-4280-b097-28b596e3c117
Grand, Maxime
659acbde-d639-42b7-9fab-52fa1b3655ff
Beaton, Alexander D.
02f38dc0-7db0-488c-af92-465275f9d584
Nightingale, Adrian
4b51311d-c6c3-40d5-a13f-ab8917031ab3
Owsianka, David
429615d0-69a0-4db0-a241-2e6854f24f7f
Slavik, Gregory J.
3244a5e0-ac7a-4f25-b203-4e9b00a68478
Connelly, Douglas
d49131bb-af38-4768-9953-7ae0b43e33c8
Cardwell, Christopher L.
9e61f59c-959d-4dac-8320-8163f2a2158a
Mowlem, Matthew
6f633ca2-298f-48ee-a025-ce52dd62124f

Clinton-Bailey, Geraldine S., Grand, Maxime, Beaton, Alexander D., Nightingale, Adrian, Owsianka, David, Slavik, Gregory J., Connelly, Douglas, Cardwell, Christopher L. and Mowlem, Matthew (2017) A Lab-on-Chip Analyzer for in situ Measurement of Soluble Reactive Phosphate: Improved phosphate blue assay and application to fluvial monitoring. Environmental Science & Technology, 51 (17), 9989–9995. (doi:10.1021/acs.est.7b01581).

Record type: Article

Abstract

Here, we present a new in situ microfluidic phosphate sensor that features an improved “phosphate blue” assay which includes polyvinylpyrrolidone in place of traditional surfactants—improving sensitivity and reducing temperature effects. The sensor features greater power economy and analytical performance relative to commercially available alternatives, with a mean power consumption of 1.8 W, a detection limit of 40 nM, a dynamic range of 0.14–10 μM, and an infield accuracy of 4 ± 4.5%. During field testing, the sensor was continuously deployed for 9 weeks in a chalk stream, revealing complex relations between flow rates and phosphate concentration that suggest changing dominance in phosphate sources. A distinct diel phosphorus signal was observed under low flow conditions, highlighting the ability of the sensor to decouple geochemical and biotic effects on phosphate dynamics in fluvial environments. This paper highlights the importance of high resolution in situ sensors in addressing the current gross under-sampling of aquatic environments.

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Accepted/In Press date: 3 August 2017
e-pub ahead of print date: 3 August 2017
Published date: 5 September 2017

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Local EPrints ID: 414280
URI: https://eprints.soton.ac.uk/id/eprint/414280
ISSN: 0013-936X
PURE UUID: bc387eb4-2d0e-485d-a9fb-970bb540d534
ORCID for Maxime Grand: ORCID iD orcid.org/0000-0001-9338-694X

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Date deposited: 21 Sep 2017 16:31
Last modified: 03 Dec 2019 05:55

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Contributors

Author: Geraldine S. Clinton-Bailey
Author: Maxime Grand ORCID iD
Author: Alexander D. Beaton
Author: David Owsianka
Author: Gregory J. Slavik
Author: Douglas Connelly
Author: Christopher L. Cardwell
Author: Matthew Mowlem

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