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Measurement of micro-scale soil deformation around roots using 4D synchrotron tomography and image correlation

Measurement of micro-scale soil deformation around roots using 4D synchrotron tomography and image correlation
Measurement of micro-scale soil deformation around roots using 4D synchrotron tomography and image correlation
This study applied time lapse (4D) synchrotron X-ray computed tomography (SRXCT) to observe micro-scale interactions between plant roots and soil. Functionally contrasting maize root tips were repeatedly imaged during ingress into soil columns of varying water content and compaction. This yielded sequences of 3D densiometric data, representing time –resolved geometric soil and root configurations at the micron-scale. These data were used as inputs for two full-field kinematic quantification methods, which enabled the analysis of 3D soil deformation around elongating roots. Discrete object tracking was used to track rigid mineral grains, whilst continuum Digital Volume Correlation (DVC) was used to track grey-level patterns within local sub-volumes. These techniques both allowed full-field soil displacements to be quantified at an intra-rhizosphere spatial sampling scale of <300 µm. Significant differences in deformation mechanisms were identified around different phenotypes under different soil conditions. A uniquely strong contrast was observed between intact and de-capped roots grown in dry, compacted soil. This provides evidence that functional traits of the root cap significantly reduce the amount of soil disturbance per unit of root elongation, with this effect being particularly significant in drier soil.
Keyes, Samuel
ed3ee62b-e257-4b92-922c-023b232e8145
Cooper, Laura
b8f4b942-cb87-4386-ab29-17bdd0f53ec1
Koebernick, Nicolai
118c4e45-02d8-42da-84c8-8ee4fac140ad
Duncan, Simon, Jack
fa8481c1-3788-41a0-a304-02515b93ef7d
McKay Fletcher, Daniel, Marcus
60e9adeb-182b-4dfd-846a-b684f8e2358e
Scotson, Callum, Paul
47901c28-548c-41cc-9cbd-f0429a24c7cb
Van Veelen, Arjen
cb6f2c8b-4671-4836-88a0-3987fd2f2d67
Sinclair, Ian
6005f6c1-f478-434e-a52d-d310c18ade0d
Roose, Tiina
3581ab5b-71e1-4897-8d88-59f13f3bccfe
Keyes, Samuel
ed3ee62b-e257-4b92-922c-023b232e8145
Cooper, Laura
b8f4b942-cb87-4386-ab29-17bdd0f53ec1
Koebernick, Nicolai
118c4e45-02d8-42da-84c8-8ee4fac140ad
Duncan, Simon, Jack
fa8481c1-3788-41a0-a304-02515b93ef7d
McKay Fletcher, Daniel, Marcus
60e9adeb-182b-4dfd-846a-b684f8e2358e
Scotson, Callum, Paul
47901c28-548c-41cc-9cbd-f0429a24c7cb
Van Veelen, Arjen
cb6f2c8b-4671-4836-88a0-3987fd2f2d67
Sinclair, Ian
6005f6c1-f478-434e-a52d-d310c18ade0d
Roose, Tiina
3581ab5b-71e1-4897-8d88-59f13f3bccfe

Keyes, Samuel, Cooper, Laura, Koebernick, Nicolai, Duncan, Simon, Jack, McKay Fletcher, Daniel, Marcus, Scotson, Callum, Paul, Van Veelen, Arjen, Sinclair, Ian and Roose, Tiina (2017) Measurement of micro-scale soil deformation around roots using 4D synchrotron tomography and image correlation. Journal of the Royal Society Interface. (doi:10.1098/rsif.2017.0560).

Record type: Article

Abstract

This study applied time lapse (4D) synchrotron X-ray computed tomography (SRXCT) to observe micro-scale interactions between plant roots and soil. Functionally contrasting maize root tips were repeatedly imaged during ingress into soil columns of varying water content and compaction. This yielded sequences of 3D densiometric data, representing time –resolved geometric soil and root configurations at the micron-scale. These data were used as inputs for two full-field kinematic quantification methods, which enabled the analysis of 3D soil deformation around elongating roots. Discrete object tracking was used to track rigid mineral grains, whilst continuum Digital Volume Correlation (DVC) was used to track grey-level patterns within local sub-volumes. These techniques both allowed full-field soil displacements to be quantified at an intra-rhizosphere spatial sampling scale of <300 µm. Significant differences in deformation mechanisms were identified around different phenotypes under different soil conditions. A uniquely strong contrast was observed between intact and de-capped roots grown in dry, compacted soil. This provides evidence that functional traits of the root cap significantly reduce the amount of soil disturbance per unit of root elongation, with this effect being particularly significant in drier soil.

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Accepted/In Press date: 12 October 2017
e-pub ahead of print date: 8 November 2017

Identifiers

Local EPrints ID: 415239
URI: http://eprints.soton.ac.uk/id/eprint/415239
PURE UUID: f3589222-8219-4053-ba1b-838f353c640b
ORCID for Samuel Keyes: ORCID iD orcid.org/0000-0002-4129-2228
ORCID for Laura Cooper: ORCID iD orcid.org/0000-0003-0198-7591
ORCID for Tiina Roose: ORCID iD orcid.org/0000-0001-8710-1063

Catalogue record

Date deposited: 03 Nov 2017 17:30
Last modified: 07 Oct 2020 06:26

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Contributors

Author: Samuel Keyes ORCID iD
Author: Laura Cooper ORCID iD
Author: Nicolai Koebernick
Author: Simon, Jack Duncan
Author: Callum, Paul Scotson
Author: Arjen Van Veelen
Author: Ian Sinclair
Author: Tiina Roose ORCID iD

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