The University of Southampton
University of Southampton Institutional Repository

The influence of a high fat diet on bone and soft tissue formation in Matrix Gla Protein knockout mice

The influence of a high fat diet on bone and soft tissue formation in Matrix Gla Protein knockout mice
The influence of a high fat diet on bone and soft tissue formation in Matrix Gla Protein knockout mice
Studies suggest bone growth and development are influenced by maternal nutrition, during intrauterine and early postnatal life. This study assessed the role of MGP and a maternal high fat diet on vitamin K-dependent proteins’ gene expression and their impact on bone formation. Knockout (KO) offspring were smaller than wild type (WT) littermates, yet possessed the same volume of intrascapular brown adipose tissue. The total proportion of body fat was reduced, but only in animals on a control diet. Lung air volume was observed to be comparable in both KO and WT animals on the same diet. The degree of aortic calcification was reduced in KO animals maintained on a HF diet. KO females on the high fat diet showed reduced cortical bone volume and thickness in the femur and tibia. Gene expression levels of GGCX and VKOR were reduced in control fed KO animals suggesting a potential link between gene expression levels of MGP, GGCX, and VKOR and total volumes of bone, calcified soft tissue, and iBAT; with implications for modulation of body length and mass. Our results confirm the important role for vitamin K in bone and calcified soft tissue, but now extend this role to include iBAT.
MGP; bone; maternal; high fat; iBAT
2045-2322
Lanham, Stuart
28fdbbef-e3b6-4fdf-bd0f-4968eeb614d6
Cagampang, Felino
7cf57d52-4a65-4554-8306-ed65226bc50e
Oreffo, Richard
ff9fff72-6855-4d0f-bfb2-311d0e8f3778
Lanham, Stuart
28fdbbef-e3b6-4fdf-bd0f-4968eeb614d6
Cagampang, Felino
7cf57d52-4a65-4554-8306-ed65226bc50e
Oreffo, Richard
ff9fff72-6855-4d0f-bfb2-311d0e8f3778

Lanham, Stuart, Cagampang, Felino and Oreffo, Richard (2018) The influence of a high fat diet on bone and soft tissue formation in Matrix Gla Protein knockout mice Scientific Reports (In Press).

Record type: Article

Abstract

Studies suggest bone growth and development are influenced by maternal nutrition, during intrauterine and early postnatal life. This study assessed the role of MGP and a maternal high fat diet on vitamin K-dependent proteins’ gene expression and their impact on bone formation. Knockout (KO) offspring were smaller than wild type (WT) littermates, yet possessed the same volume of intrascapular brown adipose tissue. The total proportion of body fat was reduced, but only in animals on a control diet. Lung air volume was observed to be comparable in both KO and WT animals on the same diet. The degree of aortic calcification was reduced in KO animals maintained on a HF diet. KO females on the high fat diet showed reduced cortical bone volume and thickness in the femur and tibia. Gene expression levels of GGCX and VKOR were reduced in control fed KO animals suggesting a potential link between gene expression levels of MGP, GGCX, and VKOR and total volumes of bone, calcified soft tissue, and iBAT; with implications for modulation of body length and mass. Our results confirm the important role for vitamin K in bone and calcified soft tissue, but now extend this role to include iBAT.

Text Nutrients MGP KO - Accepted Manuscript
Restricted to Repository staff only until 7 May 2018.
Available under License Creative Commons Attribution.
Request a copy

More information

Accepted/In Press date: 7 February 2018
Keywords: MGP; bone; maternal; high fat; iBAT

Identifiers

Local EPrints ID: 417606
URI: https://eprints.soton.ac.uk/id/eprint/417606
ISSN: 2045-2322
PURE UUID: 62e47882-7ec6-461d-aa39-dc644f72a726
ORCID for Stuart Lanham: ORCID iD orcid.org/0000-0002-4516-264X
ORCID for Richard Oreffo: ORCID iD orcid.org/0000-0001-5995-6726

Catalogue record

Date deposited: 07 Feb 2018 17:30
Last modified: 07 Feb 2018 17:30

Export record

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Library staff edit
Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of https://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×