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Establishment of the nasal microbiota in the first 18 months of life - Correlation with early onset rhinitis and wheezing

Establishment of the nasal microbiota in the first 18 months of life - Correlation with early onset rhinitis and wheezing
Establishment of the nasal microbiota in the first 18 months of life - Correlation with early onset rhinitis and wheezing
Background: the dynamic establishment of the nasal microbiota in early life influences local mucosal immune responses and the susceptibility to childhood respiratory disorders.Objective: the aim of this case-control study was to monitor, evaluate and compare the development of the nasal microbiota of infants who developed rhinitis and wheeze in the first 18 months of life with those of healthy controls.Methods: anterior nasal swabs of 122 subjects belonging to the GUSTO birth cohort were collected longitudinally over seven time points in the first 18 months of life. The nasal microbiota signatures were analyzed using 16S rRNA multiplexed pair-end sequencing from three clinical groups (1) rhinitis alone (n=28), (2) rhinitis with concomitant wheeze (n=34) and (3) healthy controls (n=60).Results: the maturation of the nasal microbiome followed distinctive patterns in infants from both rhinitis groups compared to controls. Bacterial diversity increased over the period of 18 months of life in control infants, whilst infants with rhinitis showed a decreasing trend (p<0.05). An increase in abundance of Oxalobacteraceae family (Proteobacteria phylum) and Aerococcaceae family (Firmicutes phylum) was associated with rhinitis and concomitant wheeze (adj p<0.01) whilst Corynebacteriaceae family (Actinobacteria phylum) and early colonization with Staphylococcaceae family (Firmicutes phylum) (3 weeks till 9 months) was associated with controls (adj p<0.05). The only difference between the rhinitis group and controls was a reduced abundance of Corynebacteriaceae family (adj p<0.05). Determinants of nasal microbiota succession included gender, mode of delivery, presence of siblings and infant care attendance.Conclusion: our results support the hypothesis that nasal microbiome is involved in the development of early onset rhinitis and wheeze in infants.
0091-6749
86-95
Ta, Le Duc Huy
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Yap, Gaik Chin
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Tay, Carina Jing Xuan
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Lim, A S M
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Huang, C.H.
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Chu, C.W.
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De Sessions, P.F.
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Shek, Lynette P.
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Goh, Anne
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Van Bever, Hugo
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Teoh, Oon Hoe
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Soh, J.Y.
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Thomas, Biju
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Ramamurthy, M.B.
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Goh, Daniel Y.T.
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Lay, Christophe
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Soh, S.E.
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Chan, Yiong
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Saw, Seang-Mei
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Kwek, Kenneth
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Chong, Yap-Seng
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Godfrey, Keith
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Hibberd, Martin Lloyd
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Lee, Bee Wah
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Ta, Le Duc Huy
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Yap, Gaik Chin
280a12f8-89d9-4f25-92a7-3b56cc8a5b99
Tay, Carina Jing Xuan
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Lim, A S M
bc4dd552-e444-4a10-aa64-85e103c4d216
Huang, C.H.
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Chu, C.W.
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De Sessions, P.F.
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Shek, Lynette P.
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Goh, Anne
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Van Bever, Hugo
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Teoh, Oon Hoe
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Soh, J.Y.
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Thomas, Biju
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Ramamurthy, M.B.
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Goh, Daniel Y.T.
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Lay, Christophe
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Soh, S.E.
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Chan, Yiong
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Saw, Seang-Mei
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Kwek, Kenneth
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Chong, Yap-Seng
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Godfrey, Keith
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Hibberd, Martin Lloyd
bc80ef8c-dfc8-4f3f-bdbf-5494de6679cb
Lee, Bee Wah
81ec0089-b824-4835-b908-1fc8e9f62249

Ta, Le Duc Huy, Yap, Gaik Chin, Tay, Carina Jing Xuan, Lim, A S M, Huang, C.H., Chu, C.W., De Sessions, P.F., Shek, Lynette P., Goh, Anne, Van Bever, Hugo, Teoh, Oon Hoe, Soh, J.Y., Thomas, Biju, Ramamurthy, M.B., Goh, Daniel Y.T., Lay, Christophe, Soh, S.E., Chan, Yiong, Saw, Seang-Mei, Kwek, Kenneth, Chong, Yap-Seng, Godfrey, Keith, Hibberd, Martin Lloyd and Lee, Bee Wah (2018) Establishment of the nasal microbiota in the first 18 months of life - Correlation with early onset rhinitis and wheezing. Journal of Allergy and Clinical Immunology, 142 (1), 86-95. (doi:10.1016/j.jaci.2018.01.032).

Record type: Article

Abstract

Background: the dynamic establishment of the nasal microbiota in early life influences local mucosal immune responses and the susceptibility to childhood respiratory disorders.Objective: the aim of this case-control study was to monitor, evaluate and compare the development of the nasal microbiota of infants who developed rhinitis and wheeze in the first 18 months of life with those of healthy controls.Methods: anterior nasal swabs of 122 subjects belonging to the GUSTO birth cohort were collected longitudinally over seven time points in the first 18 months of life. The nasal microbiota signatures were analyzed using 16S rRNA multiplexed pair-end sequencing from three clinical groups (1) rhinitis alone (n=28), (2) rhinitis with concomitant wheeze (n=34) and (3) healthy controls (n=60).Results: the maturation of the nasal microbiome followed distinctive patterns in infants from both rhinitis groups compared to controls. Bacterial diversity increased over the period of 18 months of life in control infants, whilst infants with rhinitis showed a decreasing trend (p<0.05). An increase in abundance of Oxalobacteraceae family (Proteobacteria phylum) and Aerococcaceae family (Firmicutes phylum) was associated with rhinitis and concomitant wheeze (adj p<0.01) whilst Corynebacteriaceae family (Actinobacteria phylum) and early colonization with Staphylococcaceae family (Firmicutes phylum) (3 weeks till 9 months) was associated with controls (adj p<0.05). The only difference between the rhinitis group and controls was a reduced abundance of Corynebacteriaceae family (adj p<0.05). Determinants of nasal microbiota succession included gender, mode of delivery, presence of siblings and infant care attendance.Conclusion: our results support the hypothesis that nasal microbiome is involved in the development of early onset rhinitis and wheeze in infants.

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Accepted/In Press date: 25 January 2018
e-pub ahead of print date: 13 February 2018
Published date: July 2018

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Local EPrints ID: 417680
URI: https://eprints.soton.ac.uk/id/eprint/417680
ISSN: 0091-6749
PURE UUID: 71c76f85-f3c6-4196-ab69-e2b39058c238
ORCID for Keith Godfrey: ORCID iD orcid.org/0000-0002-4643-0618

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Date deposited: 09 Feb 2018 17:30
Last modified: 03 Dec 2019 05:36

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Contributors

Author: Le Duc Huy Ta
Author: Gaik Chin Yap
Author: Carina Jing Xuan Tay
Author: A S M Lim
Author: C.H. Huang
Author: C.W. Chu
Author: P.F. De Sessions
Author: Lynette P. Shek
Author: Anne Goh
Author: Hugo Van Bever
Author: Oon Hoe Teoh
Author: J.Y. Soh
Author: Biju Thomas
Author: M.B. Ramamurthy
Author: Daniel Y.T. Goh
Author: Christophe Lay
Author: S.E. Soh
Author: Yiong Chan
Author: Seang-Mei Saw
Author: Kenneth Kwek
Author: Yap-Seng Chong
Author: Keith Godfrey ORCID iD
Author: Martin Lloyd Hibberd
Author: Bee Wah Lee

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