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Improving gene silencing oligonucleotides by incorporation of peptide nucleic acids

Improving gene silencing oligonucleotides by incorporation of peptide nucleic acids
Improving gene silencing oligonucleotides by incorporation of peptide nucleic acids
The use of PNAs in therapeutics is limited by its mechanism of action. PNA (peptide nucleic acid) acts as steric blocker and therefore one copy per target of these therapeutic oligonucleotides is needed. While this mechanism is very interesting in splice-switching therapeutics it falls short of Ago2 or RNase H dependent gene silencing. Although the failure of PNA to recruit these enzymes to cleave their target could be a deal breaker, the high nuclease stability, neutral backbone and high affinity of PNAs are features that could enhance efficacy of siRNAs and antisense oligonucleotides (ASOs). First, the present work discussed the design, synthesis and properties of PNAs and LNA-modified oligonucleotides, which were used to switch off a silencing modified small non-coding RNA MicAstab. Then, usage of PNAs in tandem with highly modified siRNA is discussed. The silencing activity of siRNAs containing PNA sense strand as RNA:PNA duplex was investigated. Association of PNA and siRNA was further studied and silencing activity and biophysical properties of PNA-Peptide carrier for siRNA delivery is shown. Finally, the optimisation of DNA-PNA chimeras was investigated. The synthesis of the monomers as well as the oligomerisation of LNA-DNA-PNA is described. The biophysical properties of chimeras and their ability to efficiently knock down MALAT1 RNA in cells are shown in this thesis.
University of Southampton
Debacker, Alexandre J.
8c0f0066-c6bb-4094-85d9-7a8ed619d1c3
Debacker, Alexandre J.
8c0f0066-c6bb-4094-85d9-7a8ed619d1c3
Watts, Jonathan K
c4de85ee-aaa3-4e7d-99b3-147a4de4f01c

Debacker, Alexandre J. (2017) Improving gene silencing oligonucleotides by incorporation of peptide nucleic acids. University of Southampton, Doctoral Thesis, 229pp.

Record type: Thesis (Doctoral)

Abstract

The use of PNAs in therapeutics is limited by its mechanism of action. PNA (peptide nucleic acid) acts as steric blocker and therefore one copy per target of these therapeutic oligonucleotides is needed. While this mechanism is very interesting in splice-switching therapeutics it falls short of Ago2 or RNase H dependent gene silencing. Although the failure of PNA to recruit these enzymes to cleave their target could be a deal breaker, the high nuclease stability, neutral backbone and high affinity of PNAs are features that could enhance efficacy of siRNAs and antisense oligonucleotides (ASOs). First, the present work discussed the design, synthesis and properties of PNAs and LNA-modified oligonucleotides, which were used to switch off a silencing modified small non-coding RNA MicAstab. Then, usage of PNAs in tandem with highly modified siRNA is discussed. The silencing activity of siRNAs containing PNA sense strand as RNA:PNA duplex was investigated. Association of PNA and siRNA was further studied and silencing activity and biophysical properties of PNA-Peptide carrier for siRNA delivery is shown. Finally, the optimisation of DNA-PNA chimeras was investigated. The synthesis of the monomers as well as the oligomerisation of LNA-DNA-PNA is described. The biophysical properties of chimeras and their ability to efficiently knock down MALAT1 RNA in cells are shown in this thesis.

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A_J_Debacker_PhD_Thesis_NoSign - Version of Record
Restricted to Repository staff only until 28 June 2021.
Available under License University of Southampton Thesis Licence.

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Published date: September 2017

Identifiers

Local EPrints ID: 422159
URI: http://eprints.soton.ac.uk/id/eprint/422159
PURE UUID: 14b4991d-0bb4-4137-a32f-6d67e0d4a10c

Catalogue record

Date deposited: 18 Jul 2018 16:30
Last modified: 13 Mar 2019 18:18

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