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Electric-field driven translocation of ssDNA through hydrophobic nanopores

Electric-field driven translocation of ssDNA through hydrophobic nanopores
Electric-field driven translocation of ssDNA through hydrophobic nanopores
The accurate sequencing of DNA using nanopores requires control over the speed of DNA translocation through the pores and also of the DNA conformation. Our studies show that ssDNA translocates through hourglass-shaped pores with hydrophobic constriction regions when an electric field is applied. The constriction provides a barrier to translocation and thereby slows down DNA movement through the pore compared with pores without the constriction. We show that ssDNA moves through these hydrophobic pores in an extended conformation and therefore does not form undesirable secondary structures that may affect the accuracy of partial current blockages for DNA sequencing.
DNA sequencing, hourglass pores, DNA conformation, hydrophobic constriction
1936-0851
Haynes, Taylor
1180690e-8075-457a-8bb2-d4561a59521a
Smith, Iain P.S.
312866c6-a3dd-4b15-b16e-6ff47e82ff52
Wallace, E. Jayne
d1261a27-c118-40b6-b425-f761fdd3cb32
Trick, Jemma
2ae47d42-8704-478f-822c-410eb3ba1310
Sansom, Mark S.P
388f3589-c0a1-4c95-a4c3-0cc47d576bf2
Haynes, Taylor
1180690e-8075-457a-8bb2-d4561a59521a
Smith, Iain P.S.
312866c6-a3dd-4b15-b16e-6ff47e82ff52
Wallace, E. Jayne
d1261a27-c118-40b6-b425-f761fdd3cb32
Trick, Jemma
2ae47d42-8704-478f-822c-410eb3ba1310
Sansom, Mark S.P
388f3589-c0a1-4c95-a4c3-0cc47d576bf2

Haynes, Taylor, Smith, Iain P.S., Wallace, E. Jayne, Trick, Jemma and Sansom, Mark S.P (2018) Electric-field driven translocation of ssDNA through hydrophobic nanopores. ACS Nano. (doi:10.1021/acsnano.8b03365).

Record type: Article

Abstract

The accurate sequencing of DNA using nanopores requires control over the speed of DNA translocation through the pores and also of the DNA conformation. Our studies show that ssDNA translocates through hourglass-shaped pores with hydrophobic constriction regions when an electric field is applied. The constriction provides a barrier to translocation and thereby slows down DNA movement through the pore compared with pores without the constriction. We show that ssDNA moves through these hydrophobic pores in an extended conformation and therefore does not form undesirable secondary structures that may affect the accuracy of partial current blockages for DNA sequencing.

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nanopores_DNA_revised_TH_MS_NOH - Accepted Manuscript
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Accepted/In Press date: 4 May 2018
e-pub ahead of print date: 9 July 2018
Keywords: DNA sequencing, hourglass pores, DNA conformation, hydrophobic constriction

Identifiers

Local EPrints ID: 423004
URI: http://eprints.soton.ac.uk/id/eprint/423004
ISSN: 1936-0851
PURE UUID: 7d4dc595-d30e-409d-b4d9-c1f4ed2d135e

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Date deposited: 09 Aug 2018 16:30
Last modified: 07 Oct 2020 05:39

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Contributors

Author: Taylor Haynes
Author: Iain P.S. Smith
Author: E. Jayne Wallace
Author: Jemma Trick
Author: Mark S.P Sansom

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