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Imaging microstructure of the barley rhizosphere: particle packing and root hair influences

Imaging microstructure of the barley rhizosphere: particle packing and root hair influences
Imaging microstructure of the barley rhizosphere: particle packing and root hair influences
Soil adjacent to roots has distinct structural and physical properties from bulk soil, affecting water and solute acquisition by plants. Detailed knowledge on how root activity and traits such as root hairs affect the 3D pore structure at a fine scale is scarce and often contradictory.Roots of hairless barley (Hordeum vulgare L. cv ‘Optic’) mutant (NRH) and its wildtype (WT) parent were grown in tubes of sieved (<250 μm) sandy loam soil under two different water regimes. The tubes were scanned with synchrotron based X‐ray CT to visualise pore structure at the soil‐root interface. Pore volume fraction and pore size distribution were analysed versus distance within 1 mm of the root surface.Less dense packing packing of particles at the root‐surface was hypothesised to cause the observed increased pore volume fraction immediately next to the epidermis. The pore size distribution was narrower due to a decreased fraction of larger pores. There were no statistically significant differences in pore structure between genotypes or moisture conditions.A model is proposed that describes the variation in porosity near roots taking into account soil compaction and the surface effect at the root surface.
root hairs, rhizosphere, hordeum vulgare, noninvasive, Imaging, synchotron, soil structure, particle packing
0028-646X
1878-1889
Koebernick, Nicolai
118c4e45-02d8-42da-84c8-8ee4fac140ad
Daly, Keith R.
29920932-1779-4d08-81f8-bdd898191e5a
Keyes, Samuel D.
ed3ee62b-e257-4b92-922c-023b232e8145
Bengough, Anthony G.
ac1f754e-002c-44f6-8907-8771a4cd1fd3
Brown, Lawrie K.
e9b2a56b-bb20-4aea-b8b8-eae0e9122e55
Cooper, Laura
b8f4b942-cb87-4386-ab29-17bdd0f53ec1
George, Timothy S.
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Hallett, Paul D.
2e6a4596-83d9-4aeb-bb84-025c3660a536
Naveed, Muhammad
ffa04925-7d0a-42fa-b95b-548fe3e4e2db
Raffan, Annette
65909bd1-df87-4fc1-8c15-c2395d01dd0f
Roose, Tiina
3581ab5b-71e1-4897-8d88-59f13f3bccfe
Koebernick, Nicolai
118c4e45-02d8-42da-84c8-8ee4fac140ad
Daly, Keith R.
29920932-1779-4d08-81f8-bdd898191e5a
Keyes, Samuel D.
ed3ee62b-e257-4b92-922c-023b232e8145
Bengough, Anthony G.
ac1f754e-002c-44f6-8907-8771a4cd1fd3
Brown, Lawrie K.
e9b2a56b-bb20-4aea-b8b8-eae0e9122e55
Cooper, Laura
b8f4b942-cb87-4386-ab29-17bdd0f53ec1
George, Timothy S.
6b957175-f0b0-4456-87e3-241040c31cf8
Hallett, Paul D.
2e6a4596-83d9-4aeb-bb84-025c3660a536
Naveed, Muhammad
ffa04925-7d0a-42fa-b95b-548fe3e4e2db
Raffan, Annette
65909bd1-df87-4fc1-8c15-c2395d01dd0f
Roose, Tiina
3581ab5b-71e1-4897-8d88-59f13f3bccfe

Koebernick, Nicolai, Daly, Keith R., Keyes, Samuel D., Bengough, Anthony G., Brown, Lawrie K., Cooper, Laura, George, Timothy S., Hallett, Paul D., Naveed, Muhammad, Raffan, Annette and Roose, Tiina (2019) Imaging microstructure of the barley rhizosphere: particle packing and root hair influences. New Phytologist, 221 (4), 1878-1889. (doi:10.1111/nph.15516).

Record type: Article

Abstract

Soil adjacent to roots has distinct structural and physical properties from bulk soil, affecting water and solute acquisition by plants. Detailed knowledge on how root activity and traits such as root hairs affect the 3D pore structure at a fine scale is scarce and often contradictory.Roots of hairless barley (Hordeum vulgare L. cv ‘Optic’) mutant (NRH) and its wildtype (WT) parent were grown in tubes of sieved (<250 μm) sandy loam soil under two different water regimes. The tubes were scanned with synchrotron based X‐ray CT to visualise pore structure at the soil‐root interface. Pore volume fraction and pore size distribution were analysed versus distance within 1 mm of the root surface.Less dense packing packing of particles at the root‐surface was hypothesised to cause the observed increased pore volume fraction immediately next to the epidermis. The pore size distribution was narrower due to a decreased fraction of larger pores. There were no statistically significant differences in pore structure between genotypes or moisture conditions.A model is proposed that describes the variation in porosity near roots taking into account soil compaction and the surface effect at the root surface.

Text
Koebernicketal New Phyt 2018 submitted FINAL - Accepted Manuscript
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Figure files - Imaging microstructure of the barley rhizosphere: particle packing and root hair influences - Other
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More information

Accepted/In Press date: 24 September 2018
e-pub ahead of print date: 5 October 2018
Published date: March 2019
Keywords: root hairs, rhizosphere, hordeum vulgare, noninvasive, Imaging, synchotron, soil structure, particle packing

Identifiers

Local EPrints ID: 425461
URI: https://eprints.soton.ac.uk/id/eprint/425461
ISSN: 0028-646X
PURE UUID: 330adffc-555e-4502-aeae-2cd615a2fd43
ORCID for Samuel D. Keyes: ORCID iD orcid.org/0000-0002-4129-2228
ORCID for Laura Cooper: ORCID iD orcid.org/0000-0003-0198-7591
ORCID for Tiina Roose: ORCID iD orcid.org/0000-0001-8710-1063

Catalogue record

Date deposited: 19 Oct 2018 16:30
Last modified: 14 Mar 2019 01:39

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Contributors

Author: Nicolai Koebernick
Author: Keith R. Daly
Author: Samuel D. Keyes ORCID iD
Author: Anthony G. Bengough
Author: Lawrie K. Brown
Author: Laura Cooper ORCID iD
Author: Timothy S. George
Author: Paul D. Hallett
Author: Muhammad Naveed
Author: Annette Raffan
Author: Tiina Roose ORCID iD

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