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Integrated eutopic endometrium and non-depleted serum quantitative proteomic analysis identifies candidate serological markers of endometriosis

Integrated eutopic endometrium and non-depleted serum quantitative proteomic analysis identifies candidate serological markers of endometriosis
Integrated eutopic endometrium and non-depleted serum quantitative proteomic analysis identifies candidate serological markers of endometriosis
Background: Endometriosis affects about 4% of women in the reproductive age and is associated with subfertility. The aim of the present study was to examine the quantitative proteomic profile of eutopic endometrium and serum from women with endometriosis compared to controls in order to identify candidate disease-specific serological markers.

Methods: Eutopic endometrium and serum from patients with endometriosis (n=8 for tissue and n=4 for serum) was respectively compared to endometrium and serum from females without endometriosis (n=8 for tissue and n=4 for serum) using a shotgun quantitative proteomics method. All study participants were at the proliferative phase of their menstrual cycle.

Results: At the tissue and serum level, 1,214 and 404 proteins were differentially expressed (DEPs) in eutopic endometrium and serum respectively of women with endometriosis vs. control. Gene ontology analysis showed that terms related to immune response | inflammation, cell adhesion | migration and blood coagulation were significantly enriched in the DEPs of eutopic endometrium as well as serum. Twenty-one DEPs had the same trend of differential expression in both matrices and can be further examined as potential disease- and tissue-specific serological markers of endometriosis.

Conclusions: The present in-depth proteomic profiling of eutopic endometrium and serum from women with endometriosis identified promising serological markers that can be further validated in larger cohorts for the minimally invasive diagnosis of endometriosis.

e1800153
Manousopoulou, Antigoni
9a5e4e75-cea9-4d0b-91c8-0fa2af02632f
Hamdan, Mukhri
f2846fbc-f58b-423d-af08-dc21c37dc9f0
Fotopoulos, Miltiadis
05714262-55c5-48a7-af57-397d5dd1cd6a
Garay Baquero, Diana
da9136fe-3d47-4d04-8ab3-96bfe17a773c
Teng, Jie
aa0dac71-baf9-499f-9bd4-593e917c9c8c
Garbis, Spiros
7067fd19-50c9-4d42-9611-f370289470bd
Cheong, Ying
4efbba2a-3036-4dce-82f1-8b4017952c83
Manousopoulou, Antigoni
9a5e4e75-cea9-4d0b-91c8-0fa2af02632f
Hamdan, Mukhri
f2846fbc-f58b-423d-af08-dc21c37dc9f0
Fotopoulos, Miltiadis
05714262-55c5-48a7-af57-397d5dd1cd6a
Garay Baquero, Diana
da9136fe-3d47-4d04-8ab3-96bfe17a773c
Teng, Jie
aa0dac71-baf9-499f-9bd4-593e917c9c8c
Garbis, Spiros
7067fd19-50c9-4d42-9611-f370289470bd
Cheong, Ying
4efbba2a-3036-4dce-82f1-8b4017952c83

Manousopoulou, Antigoni, Hamdan, Mukhri, Fotopoulos, Miltiadis, Garay Baquero, Diana, Teng, Jie, Garbis, Spiros and Cheong, Ying (2018) Integrated eutopic endometrium and non-depleted serum quantitative proteomic analysis identifies candidate serological markers of endometriosis. Proteomics Clinical Applications, e1800153. (doi:10.1002/prca.201800153).

Record type: Article

Abstract

Background: Endometriosis affects about 4% of women in the reproductive age and is associated with subfertility. The aim of the present study was to examine the quantitative proteomic profile of eutopic endometrium and serum from women with endometriosis compared to controls in order to identify candidate disease-specific serological markers.

Methods: Eutopic endometrium and serum from patients with endometriosis (n=8 for tissue and n=4 for serum) was respectively compared to endometrium and serum from females without endometriosis (n=8 for tissue and n=4 for serum) using a shotgun quantitative proteomics method. All study participants were at the proliferative phase of their menstrual cycle.

Results: At the tissue and serum level, 1,214 and 404 proteins were differentially expressed (DEPs) in eutopic endometrium and serum respectively of women with endometriosis vs. control. Gene ontology analysis showed that terms related to immune response | inflammation, cell adhesion | migration and blood coagulation were significantly enriched in the DEPs of eutopic endometrium as well as serum. Twenty-one DEPs had the same trend of differential expression in both matrices and can be further examined as potential disease- and tissue-specific serological markers of endometriosis.

Conclusions: The present in-depth proteomic profiling of eutopic endometrium and serum from women with endometriosis identified promising serological markers that can be further validated in larger cohorts for the minimally invasive diagnosis of endometriosis.

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06NOV18 Manousopoulou et al revision Prot Cinical Appl - Accepted Manuscript
Restricted to Repository staff only until 9 November 2019.
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Figure 1 - Accepted Manuscript
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Figure 2 - Accepted Manuscript
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More information

Accepted/In Press date: 9 November 2018
e-pub ahead of print date: 28 November 2018
Additional Information: © 2018 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Identifiers

Local EPrints ID: 426079
URI: https://eprints.soton.ac.uk/id/eprint/426079
PURE UUID: 9e26685d-a36d-4a5f-abf0-d91b148f96e1
ORCID for Spiros Garbis: ORCID iD orcid.org/0000-0002-1050-0805
ORCID for Ying Cheong: ORCID iD orcid.org/0000-0001-7687-4597

Catalogue record

Date deposited: 13 Nov 2018 17:30
Last modified: 15 Aug 2019 00:41

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