DNA – Coated gold nanoparticles for the detection of mRNA in live Hydra vulgaris animals
DNA – Coated gold nanoparticles for the detection of mRNA in live Hydra vulgaris animals
Advances in nanoparticle design have led to the development of nanoparticulate systems that can sense intracellular molecules, alter cellular processes, and release drugs to specific targets in vitro. In this work we demonstrate that oligonucleotide – coated gold nanoparticles are suitable for the detection of mRNA in live Hydra vulgaris - a model organism - without affecting the animal’s integrity. We specifically focus on the detection of Hymyc1 mRNA, which is responsible for the regulation of the balance between stem cell self-renewal and differentiation. Myc deregulation is found in more than half of human cancers, thus the ability to detect in vivo related mRNAs through innovative fluorescent systems is of outmost interest.
Moros, Maria
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Kyriazi, Maria-Eleni
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El-Sagheer, Afaf
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Brown, Tom
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Tortiglione, Claudia
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Kanaras, Antonios
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Moros, Maria
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Kyriazi, Maria-Eleni
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El-Sagheer, Afaf
05b8295a-64ad-4fdf-ad57-c34934a46c04
Brown, Tom
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Tortiglione, Claudia
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Kanaras, Antonios
667ecfdc-7647-4bd8-be03-a47bf32504c7
Moros, Maria, Kyriazi, Maria-Eleni, El-Sagheer, Afaf, Brown, Tom, Tortiglione, Claudia and Kanaras, Antonios
(2018)
DNA – Coated gold nanoparticles for the detection of mRNA in live Hydra vulgaris animals.
ACS Applied Materials and Interfaces.
(doi:10.1021/acsami.8b17846).
Abstract
Advances in nanoparticle design have led to the development of nanoparticulate systems that can sense intracellular molecules, alter cellular processes, and release drugs to specific targets in vitro. In this work we demonstrate that oligonucleotide – coated gold nanoparticles are suitable for the detection of mRNA in live Hydra vulgaris - a model organism - without affecting the animal’s integrity. We specifically focus on the detection of Hymyc1 mRNA, which is responsible for the regulation of the balance between stem cell self-renewal and differentiation. Myc deregulation is found in more than half of human cancers, thus the ability to detect in vivo related mRNAs through innovative fluorescent systems is of outmost interest.
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pure_manuscript_hydra
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Accepted/In Press date: 26 November 2018
e-pub ahead of print date: 11 December 2018
Identifiers
Local EPrints ID: 426978
URI: http://eprints.soton.ac.uk/id/eprint/426978
ISSN: 1944-8244
PURE UUID: 73ab92db-d82f-4215-8d49-2fb1e5c04bfb
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Date deposited: 19 Dec 2018 17:30
Last modified: 12 Nov 2024 05:01
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Contributors
Author:
Maria Moros
Author:
Maria-Eleni Kyriazi
Author:
Afaf El-Sagheer
Author:
Claudia Tortiglione
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