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DNA – Coated gold nanoparticles for the detection of mRNA in live Hydra vulgaris animals

DNA – Coated gold nanoparticles for the detection of mRNA in live Hydra vulgaris animals
DNA – Coated gold nanoparticles for the detection of mRNA in live Hydra vulgaris animals
Advances in nanoparticle design have led to the development of nanoparticulate systems that can sense intracellular molecules, alter cellular processes, and release drugs to specific targets in vitro. In this work we demonstrate that oligonucleotide – coated gold nanoparticles are suitable for the detection of mRNA in live Hydra vulgaris - a model organism - without affecting the animal’s integrity. We specifically focus on the detection of Hymyc1 mRNA, which is responsible for the regulation of the balance between stem cell self-renewal and differentiation. Myc deregulation is found in more than half of human cancers, thus the ability to detect in vivo related mRNAs through innovative fluorescent systems is of outmost interest.
1944-8244
Moros, Maria
3d7d9fa7-86fc-43d5-9041-ceb60114e4f4
Kyriazi, Maria-Eleni
3cfe9662-4e7f-49bc-b707-ccc2b4da6b09
El-Sagheer, Afaf
05b8295a-64ad-4fdf-ad57-c34934a46c04
Brown, Tom
a64aae36-bb30-42df-88a2-11be394e8c89
Tortiglione, Claudia
432ab93a-dc34-4745-be98-23e43af09952
Kanaras, Antonios
667ecfdc-7647-4bd8-be03-a47bf32504c7
Moros, Maria
3d7d9fa7-86fc-43d5-9041-ceb60114e4f4
Kyriazi, Maria-Eleni
3cfe9662-4e7f-49bc-b707-ccc2b4da6b09
El-Sagheer, Afaf
05b8295a-64ad-4fdf-ad57-c34934a46c04
Brown, Tom
a64aae36-bb30-42df-88a2-11be394e8c89
Tortiglione, Claudia
432ab93a-dc34-4745-be98-23e43af09952
Kanaras, Antonios
667ecfdc-7647-4bd8-be03-a47bf32504c7

Moros, Maria, Kyriazi, Maria-Eleni, El-Sagheer, Afaf, Brown, Tom, Tortiglione, Claudia and Kanaras, Antonios (2018) DNA – Coated gold nanoparticles for the detection of mRNA in live Hydra vulgaris animals. ACS Applied Materials & Interfaces. (doi:10.1021/acsami.8b17846).

Record type: Article

Abstract

Advances in nanoparticle design have led to the development of nanoparticulate systems that can sense intracellular molecules, alter cellular processes, and release drugs to specific targets in vitro. In this work we demonstrate that oligonucleotide – coated gold nanoparticles are suitable for the detection of mRNA in live Hydra vulgaris - a model organism - without affecting the animal’s integrity. We specifically focus on the detection of Hymyc1 mRNA, which is responsible for the regulation of the balance between stem cell self-renewal and differentiation. Myc deregulation is found in more than half of human cancers, thus the ability to detect in vivo related mRNAs through innovative fluorescent systems is of outmost interest.

Text
pure_manuscript_hydra - Accepted Manuscript
Restricted to Repository staff only until 11 December 2019.
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More information

Accepted/In Press date: 26 November 2018
e-pub ahead of print date: 11 December 2018

Identifiers

Local EPrints ID: 426978
URI: https://eprints.soton.ac.uk/id/eprint/426978
ISSN: 1944-8244
PURE UUID: 73ab92db-d82f-4215-8d49-2fb1e5c04bfb
ORCID for Antonios Kanaras: ORCID iD orcid.org/0000-0002-9847-6706

Catalogue record

Date deposited: 19 Dec 2018 17:30
Last modified: 15 Oct 2019 00:41

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