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Mapping bacterial biofilms on recovered orthopaedic implants by a novel agar candle dip method

Mapping bacterial biofilms on recovered orthopaedic implants by a novel agar candle dip method
Mapping bacterial biofilms on recovered orthopaedic implants by a novel agar candle dip method
While the detrimental effects of periprosthetic joint infections (PJIs) are well-known, the process of biofilm formation on orthopaedic hardware is unclear. Previous work has shown that encasement of explant hardware in agar can aid in identifying biofilms. This study tested the utility of agar “candle dip” method in detecting and mapping the location of biofilm on infected orthopedic components. Explant components from fifteen patients were rinsed, briefly submerged in agar to create a surface coating, and incubated. Larger components were coated by pipetting agar over them. After incubation, colony outgrowth on the component surface was documented (candle dip status). Data was compared with clinical laboratory results (clinical culture status) and PJI diagnosis using Musculoskeletal Infection Society criteria (MSIS status). All six patients classified as MSIS and clinical culture positive were also positive with candle dip technique. Of the nine-candle dip negative cases, four were positive and five were negative for both MSIS and clinical culture status. Candle dip may be negative in few cases due to the residual antibiotic eluting from the spacers, limiting growth of bacterial biofilms on the components. The candle dip method shows promise for biofilm mapping but requires additional testing to evaluate clinical diagnostic potential.
biofilm, implant, mapping, bacteria, orthopaedic
0903-4641
1-8
Moley, James P.
f5180f28-bc99-4862-b0c1-f6d78a2c6e34
McGrath, Mary S.
21cd2626-85f3-49ad-8baa-aabd87392575
Granger, Jeffrey F.
3237fc0c-8964-4eb1-bf5a-50cdaf30c342
Sullivan, Anne C.
de2a3aed-5fbd-411b-ad5e-c5889ceeee1e
Stoodley, Paul
08614665-92a9-4466-806e-20c6daeb483f
Dusane, Devendra H.
9a47c5eb-5587-4f1d-bfd4-8548681be2bc
Moley, James P.
f5180f28-bc99-4862-b0c1-f6d78a2c6e34
McGrath, Mary S.
21cd2626-85f3-49ad-8baa-aabd87392575
Granger, Jeffrey F.
3237fc0c-8964-4eb1-bf5a-50cdaf30c342
Sullivan, Anne C.
de2a3aed-5fbd-411b-ad5e-c5889ceeee1e
Stoodley, Paul
08614665-92a9-4466-806e-20c6daeb483f
Dusane, Devendra H.
9a47c5eb-5587-4f1d-bfd4-8548681be2bc

Moley, James P., McGrath, Mary S., Granger, Jeffrey F., Sullivan, Anne C., Stoodley, Paul and Dusane, Devendra H. (2019) Mapping bacterial biofilms on recovered orthopaedic implants by a novel agar candle dip method. Apmis, 1-8.

Record type: Article

Abstract

While the detrimental effects of periprosthetic joint infections (PJIs) are well-known, the process of biofilm formation on orthopaedic hardware is unclear. Previous work has shown that encasement of explant hardware in agar can aid in identifying biofilms. This study tested the utility of agar “candle dip” method in detecting and mapping the location of biofilm on infected orthopedic components. Explant components from fifteen patients were rinsed, briefly submerged in agar to create a surface coating, and incubated. Larger components were coated by pipetting agar over them. After incubation, colony outgrowth on the component surface was documented (candle dip status). Data was compared with clinical laboratory results (clinical culture status) and PJI diagnosis using Musculoskeletal Infection Society criteria (MSIS status). All six patients classified as MSIS and clinical culture positive were also positive with candle dip technique. Of the nine-candle dip negative cases, four were positive and five were negative for both MSIS and clinical culture status. Candle dip may be negative in few cases due to the residual antibiotic eluting from the spacers, limiting growth of bacterial biofilms on the components. The candle dip method shows promise for biofilm mapping but requires additional testing to evaluate clinical diagnostic potential.

Text
APMIS-Moley et al. 2018-Revision Submitted Accepted - Accepted Manuscript
Restricted to Repository staff only until 19 December 2019.
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More information

Accepted/In Press date: 19 December 2018
e-pub ahead of print date: 27 January 2019
Keywords: biofilm, implant, mapping, bacteria, orthopaedic

Identifiers

Local EPrints ID: 427398
URI: https://eprints.soton.ac.uk/id/eprint/427398
ISSN: 0903-4641
PURE UUID: c76a89f4-6ee1-46d5-b549-1a5b63e97dd7
ORCID for Paul Stoodley: ORCID iD orcid.org/0000-0001-6069-273X

Catalogue record

Date deposited: 15 Jan 2019 17:30
Last modified: 17 Jul 2019 16:48

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