The University of Southampton
University of Southampton Institutional Repository

A cryogel-based bioreactor for water treatment applications

A cryogel-based bioreactor for water treatment applications
A cryogel-based bioreactor for water treatment applications

The aim of this study was to develop and test a non-diffusion limited, high cell density bioreactor for biodegradation of various phenol derivatives. The bioreactor was obtained using a straightforward one-step preparation method using cryostructuration and direct cross-linking of bacteria into a 3D structured (sponge-like) macroporous cryogel composite material consisting of 11.6% (by mass) cells and 1.2–1.7% polymer, with approximately 87% water (in the material pores). The macroporous cryogel composite material, composed of live bacteria, has pore sizes in the range of 20–150 μm (confirmed by SEM and Laser Scanning Confocal Microscopy). The enzymatic activity of bacteria within the cryogel structure and the effect of freezing on the viability of the cross-linked cells was estimated by MTT assay. Cryogels based on Pseudomonas mendocina, Rhodococcus koreensis and Acinetobacter radioresistens were exploited for the effective bioremediation of phenol and m-cresol, and to a lesser extent 2-chlorophenol and 4-chlorophenol, utilising these phenolic contaminants in water as their only source of carbon. For evaluation of treatment scalability the bioreactors were prepared in plastic “Kaldnes” carriers to improve their mechanical properties and allow application in batch or fluidised bed water treatment modes.

Bacteria immobilisation, Bioremediation, Chlorophenols, Cresol, Phenol
0043-1354
324-334
Berillo, Dmitriy A.
6b5e5e57-8005-4d1e-a56d-711ee0571a51
Caplin, Jonathan L.
ec0c90c2-a7af-49da-af6f-d139a42cb631
Cundy, Andrew B.
994fdc96-2dce-40f4-b74b-dc638286eb08
Savina, Irina N.
5e5c7acd-71ef-4fb3-baaf-0b98fec91845
Berillo, Dmitriy A.
6b5e5e57-8005-4d1e-a56d-711ee0571a51
Caplin, Jonathan L.
ec0c90c2-a7af-49da-af6f-d139a42cb631
Cundy, Andrew B.
994fdc96-2dce-40f4-b74b-dc638286eb08
Savina, Irina N.
5e5c7acd-71ef-4fb3-baaf-0b98fec91845

Berillo, Dmitriy A., Caplin, Jonathan L., Cundy, Andrew B. and Savina, Irina N. (2019) A cryogel-based bioreactor for water treatment applications. Water Research, 153, 324-334. (doi:10.1016/j.watres.2019.01.028).

Record type: Article

Abstract

The aim of this study was to develop and test a non-diffusion limited, high cell density bioreactor for biodegradation of various phenol derivatives. The bioreactor was obtained using a straightforward one-step preparation method using cryostructuration and direct cross-linking of bacteria into a 3D structured (sponge-like) macroporous cryogel composite material consisting of 11.6% (by mass) cells and 1.2–1.7% polymer, with approximately 87% water (in the material pores). The macroporous cryogel composite material, composed of live bacteria, has pore sizes in the range of 20–150 μm (confirmed by SEM and Laser Scanning Confocal Microscopy). The enzymatic activity of bacteria within the cryogel structure and the effect of freezing on the viability of the cross-linked cells was estimated by MTT assay. Cryogels based on Pseudomonas mendocina, Rhodococcus koreensis and Acinetobacter radioresistens were exploited for the effective bioremediation of phenol and m-cresol, and to a lesser extent 2-chlorophenol and 4-chlorophenol, utilising these phenolic contaminants in water as their only source of carbon. For evaluation of treatment scalability the bioreactors were prepared in plastic “Kaldnes” carriers to improve their mechanical properties and allow application in batch or fluidised bed water treatment modes.

Text
1-s2.0-S004313541930082X-main - Version of Record
Download (3MB)
Text
final Water research2019 (inc. Supl figures)
Download (3MB)

More information

Accepted/In Press date: 19 January 2019
e-pub ahead of print date: 31 January 2019
Published date: 15 April 2019
Keywords: Bacteria immobilisation, Bioremediation, Chlorophenols, Cresol, Phenol

Identifiers

Local EPrints ID: 428147
URI: https://eprints.soton.ac.uk/id/eprint/428147
ISSN: 0043-1354
PURE UUID: b037afaf-07ae-446a-b8a9-adf71e02b277
ORCID for Andrew B. Cundy: ORCID iD orcid.org/0000-0003-4368-2569

Catalogue record

Date deposited: 13 Feb 2019 17:30
Last modified: 10 Dec 2019 01:33

Export record

Altmetrics

Contributors

Author: Dmitriy A. Berillo
Author: Jonathan L. Caplin
Author: Andrew B. Cundy ORCID iD
Author: Irina N. Savina

University divisions

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of https://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×