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Asymmetric confinement for defining outgrowth directionality

Asymmetric confinement for defining outgrowth directionality
Asymmetric confinement for defining outgrowth directionality
Directional connectivity is required to develop accurate in vitro models of the nervous system. This research investigated the interaction of murine neuronal outgrowths with asymmetric microstructured geometries to provide insights into the mechanisms governing unidirectional outgrowth bias. The structures were designed using edge-guidance and critical turning angle principles to study different prohibitive to permissive edge-guidance ratios. The different structures enable outgrowth in the permissive direction, while reducing outgrowth in the prohibitive direction. Outgrowth bias was probabilistic in nature, requiring multiple structures for effective unidirectional bias in primary hippocampal cultures at 14 days in vitro. Arrowhead structures with acute posterior corners were optimal, enabling 100% unidirectional outgrowth bias by virtue of re-routing and delay effects.
1473-0197
1484-1489
Holloway, Paul
59a865d9-08bc-43c7-aa00-d80509956d86
Hallinan, Grace
5549f978-4afd-4b1c-a4e1-6fe6530d3573
Hegde, Manjunath
f3512473-1b5a-47f4-9fde-2dfada91953a
Lane, Simon I.R.
44286abe-c5d5-49ef-acdd-4c935403c0f6
Deinhardt, Katrin
5f4fe23b-2317-499f-ba6d-e639a4885dc1
West, Jonathan
f1c2e060-16c3-44c0-af70-242a1c58b968
Holloway, Paul
59a865d9-08bc-43c7-aa00-d80509956d86
Hallinan, Grace
5549f978-4afd-4b1c-a4e1-6fe6530d3573
Hegde, Manjunath
f3512473-1b5a-47f4-9fde-2dfada91953a
Lane, Simon I.R.
44286abe-c5d5-49ef-acdd-4c935403c0f6
Deinhardt, Katrin
5f4fe23b-2317-499f-ba6d-e639a4885dc1
West, Jonathan
f1c2e060-16c3-44c0-af70-242a1c58b968

Holloway, Paul, Hallinan, Grace, Hegde, Manjunath, Lane, Simon I.R., Deinhardt, Katrin and West, Jonathan (2019) Asymmetric confinement for defining outgrowth directionality. Lab on a Chip, 19 (8), 1484-1489. (doi:10.1039/c9lc00078j).

Record type: Article

Abstract

Directional connectivity is required to develop accurate in vitro models of the nervous system. This research investigated the interaction of murine neuronal outgrowths with asymmetric microstructured geometries to provide insights into the mechanisms governing unidirectional outgrowth bias. The structures were designed using edge-guidance and critical turning angle principles to study different prohibitive to permissive edge-guidance ratios. The different structures enable outgrowth in the permissive direction, while reducing outgrowth in the prohibitive direction. Outgrowth bias was probabilistic in nature, requiring multiple structures for effective unidirectional bias in primary hippocampal cultures at 14 days in vitro. Arrowhead structures with acute posterior corners were optimal, enabling 100% unidirectional outgrowth bias by virtue of re-routing and delay effects.

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Holloway_RevisedArticle_withFigures_AsymmetricOutgrowth - Accepted Manuscript
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Accepted/In Press date: 17 March 2019
e-pub ahead of print date: 18 March 2019
Published date: 21 April 2019

Identifiers

Local EPrints ID: 430697
URI: http://eprints.soton.ac.uk/id/eprint/430697
ISSN: 1473-0197
PURE UUID: 2697a576-d7d6-421b-b909-da3e002a85cb
ORCID for Katrin Deinhardt: ORCID iD orcid.org/0000-0002-6473-5298
ORCID for Jonathan West: ORCID iD orcid.org/0000-0002-5709-6790

Catalogue record

Date deposited: 08 May 2019 16:30
Last modified: 26 Nov 2021 05:13

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Contributors

Author: Paul Holloway
Author: Grace Hallinan
Author: Manjunath Hegde
Author: Simon I.R. Lane
Author: Jonathan West ORCID iD

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