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The response of methanotrophs to additions of either ammonium, nitrate or urea in alpine swamp meadow soil as revealed by stable isotope probing

The response of methanotrophs to additions of either ammonium, nitrate or urea in alpine swamp meadow soil as revealed by stable isotope probing
The response of methanotrophs to additions of either ammonium, nitrate or urea in alpine swamp meadow soil as revealed by stable isotope probing
Different forms of nitrogen (N) are deposited on the Qinghai-Tibetan plateau (QTP), while their effects on the activity of methanotrophs in soil remain elusive. We constructed microcosms amended with different N fertilizers (ammonia, nitrate and urea) using the soils sampled from a swamp meadow on the QTP. The responses of active methanotrophs to different forms of nitrogen were determined by stable isotope probing with 5% 13C-methane. At the early stage of incubation, all N fertilizers, especially urea, suppressed methane oxidation compared with the control. The methane oxidation rate increased during the incubation, suggesting an adaptation and stimulation of some methanotrophs to elevated methane. At the onset of the incubation, the type II methanotrophs Methylocystis were most abundant, but decreased during the incubation and were replaced by the type Ia methanotrophs Methylomonas. Ammonia and urea had similar effects on the methanotroph communities, both characterized by an elevation in the proportion of Methylobacter and more diverse methanotroph communities. Nitrate had less effect on the methanotroph community. Our results uncovered the active methanotrophs responding to different nitrogen forms, and suggested that urea-N might have profound effects on methanotroph diversity and activity in swamp meadow soils on the QTP.
0168-6496
1-12
He, Dan
2d9082cc-b7b4-49bd-822d-16c54fdac876
Zhang, Liyan
466a7e2d-b313-425d-98a9-2776e1084e2e
Dumont, Marc
afd9f08f-bdbb-4cee-b792-1a7f000ee511
He, Jin-Sheng
d01bb3c1-216c-492a-becf-c207250c7829
Ren, Lijuan
756c2d18-1732-4543-9c1c-0944c8932b26
Chu, Haiyan
d4f0f658-2b5e-4387-9604-11842a37bcce
He, Dan
2d9082cc-b7b4-49bd-822d-16c54fdac876
Zhang, Liyan
466a7e2d-b313-425d-98a9-2776e1084e2e
Dumont, Marc
afd9f08f-bdbb-4cee-b792-1a7f000ee511
He, Jin-Sheng
d01bb3c1-216c-492a-becf-c207250c7829
Ren, Lijuan
756c2d18-1732-4543-9c1c-0944c8932b26
Chu, Haiyan
d4f0f658-2b5e-4387-9604-11842a37bcce

He, Dan, Zhang, Liyan, Dumont, Marc, He, Jin-Sheng, Ren, Lijuan and Chu, Haiyan (2019) The response of methanotrophs to additions of either ammonium, nitrate or urea in alpine swamp meadow soil as revealed by stable isotope probing. FEMS Microbiology Ecology, 95 (7), 1-12, [fiz077]. (doi:10.1093/femsec/fiz077).

Record type: Article

Abstract

Different forms of nitrogen (N) are deposited on the Qinghai-Tibetan plateau (QTP), while their effects on the activity of methanotrophs in soil remain elusive. We constructed microcosms amended with different N fertilizers (ammonia, nitrate and urea) using the soils sampled from a swamp meadow on the QTP. The responses of active methanotrophs to different forms of nitrogen were determined by stable isotope probing with 5% 13C-methane. At the early stage of incubation, all N fertilizers, especially urea, suppressed methane oxidation compared with the control. The methane oxidation rate increased during the incubation, suggesting an adaptation and stimulation of some methanotrophs to elevated methane. At the onset of the incubation, the type II methanotrophs Methylocystis were most abundant, but decreased during the incubation and were replaced by the type Ia methanotrophs Methylomonas. Ammonia and urea had similar effects on the methanotroph communities, both characterized by an elevation in the proportion of Methylobacter and more diverse methanotroph communities. Nitrate had less effect on the methanotroph community. Our results uncovered the active methanotrophs responding to different nitrogen forms, and suggested that urea-N might have profound effects on methanotroph diversity and activity in swamp meadow soils on the QTP.

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He et al. FEMS Manuscript - Accepted Manuscript
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More information

Accepted/In Press date: 23 May 2019
e-pub ahead of print date: 24 May 2019
Published date: July 2019

Identifiers

Local EPrints ID: 431628
URI: http://eprints.soton.ac.uk/id/eprint/431628
ISSN: 0168-6496
PURE UUID: bfa830bf-123c-438e-a6a1-c6b7d762d507
ORCID for Marc Dumont: ORCID iD orcid.org/0000-0002-7347-8668

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Date deposited: 11 Jun 2019 16:30
Last modified: 07 Oct 2020 04:52

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