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In vitro and ex vivo evaluation of the biological performance of sclerosing foams

In vitro and ex vivo evaluation of the biological performance of sclerosing foams
In vitro and ex vivo evaluation of the biological performance of sclerosing foams
Since the first reports on foam sclerotherapy, multiple studies have been conducted to determine the physical properties and behavior of foams, but relatively little is known about their biological effects on the endothelial cells lining the vessel wall. Moreover, a systematic comparison of the biological performance of foams produced with different methods has not been carried out yet. Herein, a 2D in vitro method was developed to compare efficacy of commercially available polidocanol injectable foam (PEM, Varithena) and physician compounded foams (PCFs). Endothelial cell attachment upon treatment with foam was quantified as an indicator of therapeutic efficacy, and was correlated with foam physical characteristics and administration conditions. An ex vivo method was also developed to establish the disruption and permeabilisation of the endothelium caused by sclerosing agents. It relied on the quantitation of extravasated bovine serum albumin conjugated to Evans Blue, as an indicator of endothelial permeability. In our series of comparisons, PEM presented a greater overall efficacy compared to PCFs, across the different biological models, which was attributed to its drainage dynamics and gas formulation. This is consistent with earlier studies that indicated superior physical cohesiveness of PEM compared to PCFs.
2045-2322
1-14
Bottaro, Elisabetta
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Paterson, Jemma, Alanna Jane
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Quercia, Luciano
a6aadcbc-1d5c-48bb-a6bf-b9ac097828c8
Zhang, Xunli
d7cf1181-3276-4da1-9150-e212b333abb1
Hill, Martyn
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Patel, Venisha A.
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Jones, Stephen A.
4c047c30-afa1-4914-92f3-7a3d8d8d765d
Lewis, Andrew L.
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Millar, Timothy
ec88510c-ad88-49f6-8b2d-4277c84c1958
Carugo, Dario
0a4be6cd-e309-4ed8-a620-20256ce01179
Bottaro, Elisabetta
6e8d35c1-e87a-4d20-a6a5-99fd44281c57
Paterson, Jemma, Alanna Jane
61d7c33a-b6f0-4d6b-9f3d-1cebc80983e1
Quercia, Luciano
a6aadcbc-1d5c-48bb-a6bf-b9ac097828c8
Zhang, Xunli
d7cf1181-3276-4da1-9150-e212b333abb1
Hill, Martyn
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Patel, Venisha A.
c9dcb951-d5f9-46f4-a109-15e5f0dfef40
Jones, Stephen A.
4c047c30-afa1-4914-92f3-7a3d8d8d765d
Lewis, Andrew L.
f604ae82-4d54-4f04-ac8f-e7bc6f1f832c
Millar, Timothy
ec88510c-ad88-49f6-8b2d-4277c84c1958
Carugo, Dario
0a4be6cd-e309-4ed8-a620-20256ce01179

Bottaro, Elisabetta, Paterson, Jemma, Alanna Jane, Quercia, Luciano, Zhang, Xunli, Hill, Martyn, Patel, Venisha A., Jones, Stephen A., Lewis, Andrew L., Millar, Timothy and Carugo, Dario (2019) In vitro and ex vivo evaluation of the biological performance of sclerosing foams. Scientific Reports, 9, 1-14, [9880]. (doi:10.1038/s41598-019-46262-0).

Record type: Article

Abstract

Since the first reports on foam sclerotherapy, multiple studies have been conducted to determine the physical properties and behavior of foams, but relatively little is known about their biological effects on the endothelial cells lining the vessel wall. Moreover, a systematic comparison of the biological performance of foams produced with different methods has not been carried out yet. Herein, a 2D in vitro method was developed to compare efficacy of commercially available polidocanol injectable foam (PEM, Varithena) and physician compounded foams (PCFs). Endothelial cell attachment upon treatment with foam was quantified as an indicator of therapeutic efficacy, and was correlated with foam physical characteristics and administration conditions. An ex vivo method was also developed to establish the disruption and permeabilisation of the endothelium caused by sclerosing agents. It relied on the quantitation of extravasated bovine serum albumin conjugated to Evans Blue, as an indicator of endothelial permeability. In our series of comparisons, PEM presented a greater overall efficacy compared to PCFs, across the different biological models, which was attributed to its drainage dynamics and gas formulation. This is consistent with earlier studies that indicated superior physical cohesiveness of PEM compared to PCFs.

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Scientific Reports - E Bottaro - accepted - Accepted Manuscript
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s41598-019-46262-0 - Version of Record
Available under License Creative Commons Attribution.
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More information

Accepted/In Press date: 21 June 2019
e-pub ahead of print date: 8 July 2019

Identifiers

Local EPrints ID: 431953
URI: http://eprints.soton.ac.uk/id/eprint/431953
ISSN: 2045-2322
PURE UUID: 09f5efca-14d4-4209-9964-e268634b5280
ORCID for Xunli Zhang: ORCID iD orcid.org/0000-0002-4375-1571
ORCID for Martyn Hill: ORCID iD orcid.org/0000-0001-6448-9448
ORCID for Timothy Millar: ORCID iD orcid.org/0000-0002-4539-2445

Catalogue record

Date deposited: 03 Jul 2019 16:30
Last modified: 22 Nov 2021 02:55

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Contributors

Author: Elisabetta Bottaro
Author: Jemma, Alanna Jane Paterson
Author: Luciano Quercia
Author: Xunli Zhang ORCID iD
Author: Martyn Hill ORCID iD
Author: Venisha A. Patel
Author: Stephen A. Jones
Author: Andrew L. Lewis
Author: Timothy Millar ORCID iD
Author: Dario Carugo

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