Staphylococcus aureus internalisation in mast cells in nasal polyps - characterisation of interactions and potential mechanisms
Staphylococcus aureus internalisation in mast cells in nasal polyps - characterisation of interactions and potential mechanisms
Background: Chronic rhinosinusitis (CRS) with nasal polyps is a common chronic condition. The exact cause of nasal polyps remains unknown. Recently, we made the novel observation of intracellular localization of Staphylococcus aureus within mast cells in nasal polyps. Objective: This follow-up study aimed to further characterize interactions between S aureus and mast cells in this setting and elucidate potential internalization mechanisms with particular emphasis on the role of staphylococcal enterotoxin B (SEB). Methods: A prospective study was performed using an explant tissue model with ex vivo inferior turbinate mucosa obtained from patients with chronic rhinosinusitis with nasal polyps (n = 7) and patients without CRS (n = 5). Immunohistochemistry was used to characterize S aureus uptake into mast cells and investigate the effects of SEB on this process. An in vitro cell-culture model was used to investigate mast cell–S aureus interactions by using a combination of fluorescent in situ hybridization, confocal laser scanning microscopy, scanning electron microscopy, transmission electron microscopy, and proliferation assays. Results: S aureus was captured by extracellular traps and entered mast cells through phagocytosis. Proliferating intracellular S aureus led to the expansion and eventual rupture of mast cells, resulting in release of viable S aureus into the extracellular space. The presence of SEB appeared to promote internalization of S aureus into mast cells. Conclusion: This study provides new insights into the interactions between S aureus and mast cells, including the internalization process, and demonstrates a prominent role for SEB in promoting uptake of the bacteria into these cells.
Chronic rhinosinusitis, Staphylococcus aureus, bacterial superantigens, chronic rhinosinusitis with nasal polyps, intracellular bacteria, mast cells, nasal polyps, staphylococcal enterotoxin B
147-159
Hayes, Stephen
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Biggs, Timothy Charles
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Goldie, Simon Patrick
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Harries, Philip G.
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Walls, Andrew
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Allan, Raymond
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Pender, Sylvia
62528b03-ec42-41bb-80fe-48454c2c5242
Salib, Rami
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3 January 2020
Hayes, Stephen
7c6543a2-17a4-4629-91bb-bb64965df66a
Biggs, Timothy Charles
887596b4-f2ad-4067-8f01-5f2cfe1ae394
Goldie, Simon Patrick
0bf2f8c9-290b-403f-bf52-5602708810e9
Harries, Philip G.
df33be64-b580-4fdf-ac4d-4c842b55d753
Walls, Andrew
aaa7e455-0562-4b4c-94f5-ec29c74b1bfe
Allan, Raymond
390a7d0a-38e1-410a-8dfe-c8ef8408f5e1
Pender, Sylvia
62528b03-ec42-41bb-80fe-48454c2c5242
Salib, Rami
d6fde1c1-5b5e-43f7-ae1c-42cce6a0c9fc
Hayes, Stephen, Biggs, Timothy Charles, Goldie, Simon Patrick, Harries, Philip G., Walls, Andrew, Allan, Raymond, Pender, Sylvia and Salib, Rami
(2020)
Staphylococcus aureus internalisation in mast cells in nasal polyps - characterisation of interactions and potential mechanisms.
Journal of Allergy and Clinical Immunology, 145 (1), .
(doi:10.1016/j.jaci.2019.06.013).
Abstract
Background: Chronic rhinosinusitis (CRS) with nasal polyps is a common chronic condition. The exact cause of nasal polyps remains unknown. Recently, we made the novel observation of intracellular localization of Staphylococcus aureus within mast cells in nasal polyps. Objective: This follow-up study aimed to further characterize interactions between S aureus and mast cells in this setting and elucidate potential internalization mechanisms with particular emphasis on the role of staphylococcal enterotoxin B (SEB). Methods: A prospective study was performed using an explant tissue model with ex vivo inferior turbinate mucosa obtained from patients with chronic rhinosinusitis with nasal polyps (n = 7) and patients without CRS (n = 5). Immunohistochemistry was used to characterize S aureus uptake into mast cells and investigate the effects of SEB on this process. An in vitro cell-culture model was used to investigate mast cell–S aureus interactions by using a combination of fluorescent in situ hybridization, confocal laser scanning microscopy, scanning electron microscopy, transmission electron microscopy, and proliferation assays. Results: S aureus was captured by extracellular traps and entered mast cells through phagocytosis. Proliferating intracellular S aureus led to the expansion and eventual rupture of mast cells, resulting in release of viable S aureus into the extracellular space. The presence of SEB appeared to promote internalization of S aureus into mast cells. Conclusion: This study provides new insights into the interactions between S aureus and mast cells, including the internalization process, and demonstrates a prominent role for SEB in promoting uptake of the bacteria into these cells.
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More information
Accepted/In Press date: 4 June 2019
e-pub ahead of print date: 27 June 2019
Published date: 3 January 2020
Keywords:
Chronic rhinosinusitis, Staphylococcus aureus, bacterial superantigens, chronic rhinosinusitis with nasal polyps, intracellular bacteria, mast cells, nasal polyps, staphylococcal enterotoxin B
Identifiers
Local EPrints ID: 432078
URI: http://eprints.soton.ac.uk/id/eprint/432078
ISSN: 0091-6749
PURE UUID: 64c88634-7319-4447-9ed9-597eed18277d
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Date deposited: 01 Jul 2019 16:30
Last modified: 16 Mar 2024 07:58
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Contributors
Author:
Stephen Hayes
Author:
Timothy Charles Biggs
Author:
Simon Patrick Goldie
Author:
Philip G. Harries
Author:
Raymond Allan
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