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In vivo kinetic study of the materno-fetal fatty acid transfer in obese and normal weight pregnant women

In vivo kinetic study of the materno-fetal fatty acid transfer in obese and normal weight pregnant women
In vivo kinetic study of the materno-fetal fatty acid transfer in obese and normal weight pregnant women

We analyse for the first time the in vivo materno‐fetal kinetic transfer of fatty acids (FA) labelled with stable isotopes in control and obese (OB) pregnant women. Labelled FA with a similar metabolism (stearic acid: 13C‐SA; palmitic acid: 13C‐PA; oleic acid: 13C‐OA) were orally administered at −4 h, −8 h and −12 h, respectively prior to elective caesarean section to 10 pregnant women with a body mass index >30 (OB) and 10 with a body mass index in the range 20–25 (NW). Placenta, venous and arterial cord blood were collected obtaining a wide range of FA enrichments. A combined experimental and computational modelling analysis was applied. FA fractional synthesis rate (FSR) in placenta was 11–12% h–1. No differences were observed between NW and normo‐lipidemic OB. It was not possible to estimate FA FSR in cord blood with this oral bolus dose approach. Computational modelling demonstrated a good fit to the data when all maternal plasma lipid classes were included but not with modelling based only on the non‐esterified FA fraction. The estimated materno‐fetal 13C‐FA transfer was ∼1%. In conclusion, our approach using multiple 13C‐FA tracers allowed us to estimated FSR in placental/maternal plasma but not in fetal/maternal compartments. Computational modelling showed a consistent time course of placental 13C‐FA transfer and predicted total fetal FA accumulation during the experiment. We conclude that, in addition to non‐esterified FA fraction in the maternal circulation, maternal plasma very low‐density lipoprotein and other lipoproteins are important contributors to placental FA transfer to the fetus.

0022-3751
4959-4973
Gázquez, Antonio
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Prieto-Sánchez, María T
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Blanco-Carnero, José E
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van Harskamp, Dewi
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Perazzolo, Simone
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Oosterink, J Efraim
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Demmelmair, Hans
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Schierbeek, Henk
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Sengers, Bram G
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Lewis, Rohan M
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van Goudoever, Johannes B
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Koletzko, Berthold
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Larqué, Elvira
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Gázquez, Antonio
f1519dc3-1e5a-45b5-8497-467282cadef8
Prieto-Sánchez, María T
9ad36077-4f8b-4898-8c57-2c1b8f27e081
Blanco-Carnero, José E
7d9c9652-8fa8-47a2-bf59-672029d36690
van Harskamp, Dewi
24dfb21e-e35b-4fee-9643-c8d1cfdb75bb
Perazzolo, Simone
bc5e3f83-6eb6-4ed6-9173-860a2d03817d
Oosterink, J Efraim
f9392f0a-5ad9-4ca5-9379-1844233534e4
Demmelmair, Hans
ad6f4447-024d-4f20-a71c-62cb276a8255
Schierbeek, Henk
e480e7ce-71a3-4b23-ab70-ca027a169fa9
Sengers, Bram G
d6b771b1-4ede-48c5-9644-fa86503941aa
Lewis, Rohan M
caaeb97d-ea69-4f7b-8adb-5fa25e2d3502
van Goudoever, Johannes B
0a6f725d-e537-4f6b-8bff-a7d339a4f162
Koletzko, Berthold
1932e5e8-b045-4e48-aa1e-5e4ea6803a69
Larqué, Elvira
a79cd916-3984-49ba-bb7d-f7e511200482

Gázquez, Antonio, Prieto-Sánchez, María T, Blanco-Carnero, José E, van Harskamp, Dewi, Perazzolo, Simone, Oosterink, J Efraim, Demmelmair, Hans, Schierbeek, Henk, Sengers, Bram G, Lewis, Rohan M, van Goudoever, Johannes B, Koletzko, Berthold and Larqué, Elvira (2019) In vivo kinetic study of the materno-fetal fatty acid transfer in obese and normal weight pregnant women. The Journal of Physiology, 597 (19), 4959-4973. (doi:10.1113/JP278146).

Record type: Article

Abstract

We analyse for the first time the in vivo materno‐fetal kinetic transfer of fatty acids (FA) labelled with stable isotopes in control and obese (OB) pregnant women. Labelled FA with a similar metabolism (stearic acid: 13C‐SA; palmitic acid: 13C‐PA; oleic acid: 13C‐OA) were orally administered at −4 h, −8 h and −12 h, respectively prior to elective caesarean section to 10 pregnant women with a body mass index >30 (OB) and 10 with a body mass index in the range 20–25 (NW). Placenta, venous and arterial cord blood were collected obtaining a wide range of FA enrichments. A combined experimental and computational modelling analysis was applied. FA fractional synthesis rate (FSR) in placenta was 11–12% h–1. No differences were observed between NW and normo‐lipidemic OB. It was not possible to estimate FA FSR in cord blood with this oral bolus dose approach. Computational modelling demonstrated a good fit to the data when all maternal plasma lipid classes were included but not with modelling based only on the non‐esterified FA fraction. The estimated materno‐fetal 13C‐FA transfer was ∼1%. In conclusion, our approach using multiple 13C‐FA tracers allowed us to estimated FSR in placental/maternal plasma but not in fetal/maternal compartments. Computational modelling showed a consistent time course of placental 13C‐FA transfer and predicted total fetal FA accumulation during the experiment. We conclude that, in addition to non‐esterified FA fraction in the maternal circulation, maternal plasma very low‐density lipoprotein and other lipoproteins are important contributors to placental FA transfer to the fetus.

Text
Gázquez A. et al. J Physiology_R1 Authors accepted - Accepted Manuscript
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More information

Accepted/In Press date: 21 June 2019
e-pub ahead of print date: 9 July 2019
Published date: 30 September 2019

Identifiers

Local EPrints ID: 432657
URI: http://eprints.soton.ac.uk/id/eprint/432657
ISSN: 0022-3751
PURE UUID: ef02bf55-e2eb-4c2a-970e-8cc1f9a52c2a
ORCID for Bram G Sengers: ORCID iD orcid.org/0000-0001-5859-6984
ORCID for Rohan M Lewis: ORCID iD orcid.org/0000-0003-4044-9104

Catalogue record

Date deposited: 07 Aug 2019 16:30
Last modified: 26 Nov 2021 06:51

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Contributors

Author: Antonio Gázquez
Author: María T Prieto-Sánchez
Author: José E Blanco-Carnero
Author: Dewi van Harskamp
Author: Simone Perazzolo
Author: J Efraim Oosterink
Author: Hans Demmelmair
Author: Henk Schierbeek
Author: Bram G Sengers ORCID iD
Author: Rohan M Lewis ORCID iD
Author: Johannes B van Goudoever
Author: Berthold Koletzko
Author: Elvira Larqué

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