Analysis of the microbial communities in soils of different ages following volcanic eruptions: Microbial communities in volcanic soils
Analysis of the microbial communities in soils of different ages following volcanic eruptions: Microbial communities in volcanic soils
Volcanism is a primary process of land formation. It provides a model for understanding soil-forming processes and the role of pioneer bacteria and/or archaea as early colonizers in those new environments. The objective of this study was to identify the microbial communities involved in soil formation. DNA was extracted from soil samples from the Llaima volcano in Chile at sites destroyed by lava in different centuries (1640, 1751, and 1957). Bacterial and archaeal 16S rRNA genes were analyzed using quantitative polymerase chain reaction (qPCR) and Illumina MiSeq sequencing. Results showed that microbial diversity increased with soil age, particularly between the 1751 and 1640 soils. For archaeal communities, Thaumarchaeota was detected in similar abundances in all soils, but Euryarchaeota was rare in the older soils. The analysis of bacterial 16S rRNA genes showed high abundances of Chloroflexi (37%), Planctomycetes (18%), and Verrucomicrobia (10%) in the youngest soil. Proteobacteria and Acidobacteria were highly abundant in the older soils (16% in 1640 and 15% in 1751 for Acidobacteria; 38% in 1640 and 27% in 1751 for Proteobacteria). The microbial profiles in the youngest soils were unusual, with a high abundance of bacteria belonging to the order Ktedonobacterales (Chloroflexi) in the 1957 soil (37%) compared with the 1751 (18%) and 1640 (7%) soils. In this study, we show that there is a gradual establishment of the microbial community in volcanic soils following an eruption and that specific microbial groups can colonize during the early stages of recovery.
volcanic soils, soil formation, Ktedonobacterales, 16S rRNA gene, high-throughput sequencing
126-134
Hernandez Garcia, Marcela
e73477e7-cf3e-4f50-97c8-4494c5b05cd0
Calabi-Floody, Marcela
1dd27c78-c070-4168-96ff-dbdc6da09257
Conrad, Ralf
b63adcc7-abe3-4e99-9ce6-20f1cc671d96
Dumont, Marc
afd9f08f-bdbb-4cee-b792-1a7f000ee511
February 2020
Hernandez Garcia, Marcela
e73477e7-cf3e-4f50-97c8-4494c5b05cd0
Calabi-Floody, Marcela
1dd27c78-c070-4168-96ff-dbdc6da09257
Conrad, Ralf
b63adcc7-abe3-4e99-9ce6-20f1cc671d96
Dumont, Marc
afd9f08f-bdbb-4cee-b792-1a7f000ee511
Hernandez Garcia, Marcela, Calabi-Floody, Marcela, Conrad, Ralf and Dumont, Marc
(2020)
Analysis of the microbial communities in soils of different ages following volcanic eruptions: Microbial communities in volcanic soils.
Pedosphere, 30 (1), .
(doi:10.1016/S1002-0160(19)60823-4).
Abstract
Volcanism is a primary process of land formation. It provides a model for understanding soil-forming processes and the role of pioneer bacteria and/or archaea as early colonizers in those new environments. The objective of this study was to identify the microbial communities involved in soil formation. DNA was extracted from soil samples from the Llaima volcano in Chile at sites destroyed by lava in different centuries (1640, 1751, and 1957). Bacterial and archaeal 16S rRNA genes were analyzed using quantitative polymerase chain reaction (qPCR) and Illumina MiSeq sequencing. Results showed that microbial diversity increased with soil age, particularly between the 1751 and 1640 soils. For archaeal communities, Thaumarchaeota was detected in similar abundances in all soils, but Euryarchaeota was rare in the older soils. The analysis of bacterial 16S rRNA genes showed high abundances of Chloroflexi (37%), Planctomycetes (18%), and Verrucomicrobia (10%) in the youngest soil. Proteobacteria and Acidobacteria were highly abundant in the older soils (16% in 1640 and 15% in 1751 for Acidobacteria; 38% in 1640 and 27% in 1751 for Proteobacteria). The microbial profiles in the youngest soils were unusual, with a high abundance of bacteria belonging to the order Ktedonobacterales (Chloroflexi) in the 1957 soil (37%) compared with the 1751 (18%) and 1640 (7%) soils. In this study, we show that there is a gradual establishment of the microbial community in volcanic soils following an eruption and that specific microbial groups can colonize during the early stages of recovery.
Text
HernandezM_pedos201901021_final_version29jul19
- Accepted Manuscript
More information
Accepted/In Press date: 19 March 2019
e-pub ahead of print date: 14 December 2019
Published date: February 2020
Additional Information:
Funding Information:
The first author acknowledges the research fellowship from the Alexander von Humboldt Foundation and the Max Planck Society, Germany.
Publisher Copyright:
© 2020 Soil Science Society of China
Keywords:
volcanic soils, soil formation, Ktedonobacterales, 16S rRNA gene, high-throughput sequencing
Identifiers
Local EPrints ID: 433027
URI: http://eprints.soton.ac.uk/id/eprint/433027
ISSN: 1002-0160
PURE UUID: 36c2a84e-67f2-4924-beeb-79f837aa8a28
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Date deposited: 06 Aug 2019 16:31
Last modified: 16 Mar 2024 07:55
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Contributors
Author:
Marcela Hernandez Garcia
Author:
Marcela Calabi-Floody
Author:
Ralf Conrad
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