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Myo-inositol alters C-labelled fatty acid metabolism in human placental explants

Myo-inositol alters C-labelled fatty acid metabolism in human placental explants
Myo-inositol alters C-labelled fatty acid metabolism in human placental explants
We postulate that myo-inositol, a proposed intervention for gestational-diabetes, affects transplacental lipid supply to the fetus. We investigated the effect of myo-inositol on fatty-acid processing in human placental-explants from uncomplicated pregnancies. Explants were incubated with 13C-labeled palmitic-acid, 13C-oleic-acid and 13C-docosahexaenoic-acid across a range of myo-inositol concentrations for 24 h and 48 h. The incorporation of labeled-fatty-acids into individual lipids was quantified by liquid-chromatography-mass-spectrometry. At 24 h, myo-inositol increased the amount of 13C-palmitic-acid and 13C-oleic-acid labeled lipids (median fold-change relative to control=1). Significant effects were seen with 30 µM myo-inositol (physiological) for 13C-palmitic-acid-lysophosphatidylcholines (1.26) and 13C-palmitic-acid-phosphatidylethanolamines (1.17). At 48 h, myo-inositol addition increased 13C-oleic-acid-lipids but decreased 13C-palmitic-acid and 13C-docosahexaenoic-acid lipids. Significant effects were seen with 30 µM myo-inositol for 13C-oleic-acid-phosphatidylcholines (1.25), 13C-oleic-acid-phosphatidylethanolamines (1.37) and 13C-oleic-acid-triacylglycerols (1.32) and with 100 µM myo-inositol for 13C-docosahexaenoic-acid-triacylglycerols (0.78). Lipids labeled with the same 13C-fatty-acid showed similar responses when tested at the same time-point, suggesting myo-inositol alters upstream processes such as fatty-acid uptake or activation. Myo-inositol supplementation may alter placental lipid physiology with unknown clinical consequences.
0022-0795
73-84
Watkins, Oliver C.
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Islam, Mohammed Omedul
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Selvam, Preben
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Pillai, Reshma Appukuttan
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Cazenave-Gassiot, Amaury
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Bendt, Anne K.
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Karmani, Neerja
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Godfrey, Keith
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Lewis, Rohan
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Wenk, Markus R.
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Chan, Shiao-Yng
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Watkins, Oliver C.
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Islam, Mohammed Omedul
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Selvam, Preben
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Pillai, Reshma Appukuttan
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Cazenave-Gassiot, Amaury
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Bendt, Anne K.
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Karmani, Neerja
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Godfrey, Keith
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Lewis, Rohan
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Wenk, Markus R.
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Chan, Shiao-Yng
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Watkins, Oliver C., Islam, Mohammed Omedul, Selvam, Preben, Pillai, Reshma Appukuttan, Cazenave-Gassiot, Amaury, Bendt, Anne K., Karmani, Neerja, Godfrey, Keith, Lewis, Rohan, Wenk, Markus R. and Chan, Shiao-Yng (2019) Myo-inositol alters C-labelled fatty acid metabolism in human placental explants. Journal of Endocrinology, 243 (1), 73-84. (doi:10.1530/JOE-19-0267).

Record type: Article

Abstract

We postulate that myo-inositol, a proposed intervention for gestational-diabetes, affects transplacental lipid supply to the fetus. We investigated the effect of myo-inositol on fatty-acid processing in human placental-explants from uncomplicated pregnancies. Explants were incubated with 13C-labeled palmitic-acid, 13C-oleic-acid and 13C-docosahexaenoic-acid across a range of myo-inositol concentrations for 24 h and 48 h. The incorporation of labeled-fatty-acids into individual lipids was quantified by liquid-chromatography-mass-spectrometry. At 24 h, myo-inositol increased the amount of 13C-palmitic-acid and 13C-oleic-acid labeled lipids (median fold-change relative to control=1). Significant effects were seen with 30 µM myo-inositol (physiological) for 13C-palmitic-acid-lysophosphatidylcholines (1.26) and 13C-palmitic-acid-phosphatidylethanolamines (1.17). At 48 h, myo-inositol addition increased 13C-oleic-acid-lipids but decreased 13C-palmitic-acid and 13C-docosahexaenoic-acid lipids. Significant effects were seen with 30 µM myo-inositol for 13C-oleic-acid-phosphatidylcholines (1.25), 13C-oleic-acid-phosphatidylethanolamines (1.37) and 13C-oleic-acid-triacylglycerols (1.32) and with 100 µM myo-inositol for 13C-docosahexaenoic-acid-triacylglycerols (0.78). Lipids labeled with the same 13C-fatty-acid showed similar responses when tested at the same time-point, suggesting myo-inositol alters upstream processes such as fatty-acid uptake or activation. Myo-inositol supplementation may alter placental lipid physiology with unknown clinical consequences.

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Myo-inositol alters placental lipid metabolism_J_Endo_ 17_7_19_ clean - Accepted Manuscript
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Accepted/In Press date: 12 August 2019
e-pub ahead of print date: 12 August 2019
Published date: October 2019

Identifiers

Local EPrints ID: 433883
URI: http://eprints.soton.ac.uk/id/eprint/433883
ISSN: 0022-0795
PURE UUID: 21dfbf63-4624-4503-964f-df4277f82a92
ORCID for Keith Godfrey: ORCID iD orcid.org/0000-0002-4643-0618
ORCID for Rohan Lewis: ORCID iD orcid.org/0000-0003-4044-9104

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Date deposited: 05 Sep 2019 16:30
Last modified: 10 Jan 2022 05:25

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Contributors

Author: Oliver C. Watkins
Author: Mohammed Omedul Islam
Author: Preben Selvam
Author: Reshma Appukuttan Pillai
Author: Amaury Cazenave-Gassiot
Author: Anne K. Bendt
Author: Neerja Karmani
Author: Keith Godfrey ORCID iD
Author: Rohan Lewis ORCID iD
Author: Markus R. Wenk
Author: Shiao-Yng Chan

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