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Stabilizing gold nanoparticles for use in X-ray computed tomography imaging of soil systems

Stabilizing gold nanoparticles for use in X-ray computed tomography imaging of soil systems
Stabilizing gold nanoparticles for use in X-ray computed tomography imaging of soil systems
This investigation establishes a system of gold nanoparticles that show good colloidal stability as an X-ray computed tomography (XCT) contrast agent under soil conditions. Gold nanoparticles offer numerous beneficial traits for experiments in biology including: comparatively minimal phytotoxicity, X-ray attenuation of the material and the capacity for functionalisation. However, soil salinity, acidity and surface charges can induce aggregation and destabilise gold nanoparticles, hence in biomedical applications polymer coatings are commonly applied to gold nanoparticles to enhance stability in the in vivo environment. Here we first demonstrate non-coated nanoparticles aggregate in soil-water solutions. We then show coating with a polyethylene glycol (PEG) layer prevents this aggregation. To demonstrate this, PEG-coated nanoparticles were drawn through flow columns containing soil and were shown to be stable; this is in contrast with control experiments using silica and alumina-packed columns. We further determined that a suspension of coated gold nanoparticles which fully saturated soil maintained stability over at least five days. Finally, we used time resolved XCT imaging and image based models to approximate nanoparticle diffusion as similar to that of other typical plant nutrients diffusing in water. Together, these results establish the PEGylated gold nanoparticles as potential contrast agents for XCT imaging in soil.
Gold, Nanoparticle, soil, x-ray, contrast, Stability
2054-5703
Scotson, Callum, Paul
47901c28-548c-41cc-9cbd-f0429a24c7cb
Munoz-Hernando, Maria
ca1fd5da-8061-4998-b9f3-c0c505046281
Duncan, Simon J
5f6ded7d-ae87-4c14-80c9-6f64e306859a
Ruiz, Siul Aljadi
d79b3b82-7c0d-47cc-9616-11d29e6a41bd
Keyes, Samuel
ed3ee62b-e257-4b92-922c-023b232e8145
Van Veelen, Arjen
cb6f2c8b-4671-4836-88a0-3987fd2f2d67
Dunlop, Iain
12e19821-0a87-41f4-8c9a-d06e520b13ad
Roose, Tiina
3581ab5b-71e1-4897-8d88-59f13f3bccfe
Scotson, Callum, Paul
47901c28-548c-41cc-9cbd-f0429a24c7cb
Munoz-Hernando, Maria
ca1fd5da-8061-4998-b9f3-c0c505046281
Duncan, Simon J
5f6ded7d-ae87-4c14-80c9-6f64e306859a
Ruiz, Siul Aljadi
d79b3b82-7c0d-47cc-9616-11d29e6a41bd
Keyes, Samuel
ed3ee62b-e257-4b92-922c-023b232e8145
Van Veelen, Arjen
cb6f2c8b-4671-4836-88a0-3987fd2f2d67
Dunlop, Iain
12e19821-0a87-41f4-8c9a-d06e520b13ad
Roose, Tiina
3581ab5b-71e1-4897-8d88-59f13f3bccfe

Scotson, Callum, Paul, Munoz-Hernando, Maria, Duncan, Simon J, Ruiz, Siul Aljadi, Keyes, Samuel, Van Veelen, Arjen, Dunlop, Iain and Roose, Tiina (2019) Stabilizing gold nanoparticles for use in X-ray computed tomography imaging of soil systems. Royal Society Open Science, 6 (10). (doi:10.1098/rsos.190769).

Record type: Article

Abstract

This investigation establishes a system of gold nanoparticles that show good colloidal stability as an X-ray computed tomography (XCT) contrast agent under soil conditions. Gold nanoparticles offer numerous beneficial traits for experiments in biology including: comparatively minimal phytotoxicity, X-ray attenuation of the material and the capacity for functionalisation. However, soil salinity, acidity and surface charges can induce aggregation and destabilise gold nanoparticles, hence in biomedical applications polymer coatings are commonly applied to gold nanoparticles to enhance stability in the in vivo environment. Here we first demonstrate non-coated nanoparticles aggregate in soil-water solutions. We then show coating with a polyethylene glycol (PEG) layer prevents this aggregation. To demonstrate this, PEG-coated nanoparticles were drawn through flow columns containing soil and were shown to be stable; this is in contrast with control experiments using silica and alumina-packed columns. We further determined that a suspension of coated gold nanoparticles which fully saturated soil maintained stability over at least five days. Finally, we used time resolved XCT imaging and image based models to approximate nanoparticle diffusion as similar to that of other typical plant nutrients diffusing in water. Together, these results establish the PEGylated gold nanoparticles as potential contrast agents for XCT imaging in soil.

Text
Revised_Stabilising_Gold_Nanoparticles_RSOS_Manuscript - Accepted Manuscript
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More information

Accepted/In Press date: 25 September 2019
e-pub ahead of print date: 16 October 2019
Published date: October 2019
Keywords: Gold, Nanoparticle, soil, x-ray, contrast, Stability

Identifiers

Local EPrints ID: 434810
URI: http://eprints.soton.ac.uk/id/eprint/434810
ISSN: 2054-5703
PURE UUID: fe48ea82-89fd-404c-85d9-e0cbf4d0aeb3
ORCID for Samuel Keyes: ORCID iD orcid.org/0000-0002-4129-2228
ORCID for Tiina Roose: ORCID iD orcid.org/0000-0001-8710-1063

Catalogue record

Date deposited: 10 Oct 2019 16:30
Last modified: 07 Oct 2020 01:59

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