Complete killing of agar lawn biofilms by systematic spacing of antibiotic-loaded calcium sulfate beads
Complete killing of agar lawn biofilms by systematic spacing of antibiotic-loaded calcium sulfate beads
Background: Pseudomonas aeruginosa (PA) and Staphylococcus aureus (SA) are the major causative agents of acute and chronic infections. Antibiotic-loaded calcium sulfate beads (ALCSB) are used in the management of musculoskeletal infections such as periprosthetic joint infections (PJI).
Methods: To determine whether the number and spatial distribution are important factors to totally eradicate biofilms, ALCSBs containing vancomycin and tobramycin were placed on 24h agar lawn biofilms as a single bead in the center, or as 16 beads placed as four clusters of four, a ring around the edge and as a group in the center or 19 beads evenly across the plate. Bioluminescence was used to assess spatial metabolic activity in real time. Replica plating was used to assess viability.
Results: For both strains antibiotics released from the beads completely killed biofilm bacteria in a zone immediately adjacent to each bead. However, for PA extended incubation revealed the emergence of resistant colony phenotypes between the zone of eradication and the background lawn. The rate of biofilm clearing was greater when the beads were distributed evenly over the plate.
Conclusions: Both number and distribution pattern of ALCSB are important to ensure adequate coverage of antibiotics required to eradicate biofilms.
antibiotic tolerance, biofilm, antibiotic-loaded bone cement, Pseudomonas, Staphylococcus, persister, periprosthetic joint infection
1-14
Dusane, Devendra H.
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Brooks, Jacob R.
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Sindeldecker, Devin
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Peters, Casey W.
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Li, Anthony
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Farrar, Nicholas R.
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Diamond, Scott M.
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Knecht, Cory S.
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Plaut, Roger D.
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Delury, Craig
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Aiken, Sean S.
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Laycock, Phillip A.
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Granger, Jeffrey F.
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Stoodley, Paul
08614665-92a9-4466-806e-20c6daeb483f
2019
Dusane, Devendra H.
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Brooks, Jacob R.
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Sindeldecker, Devin
53899a0e-6c67-40e4-813d-ce3e907f3ee8
Peters, Casey W.
7c4253bd-aa41-42f3-a253-9da8bd3acb13
Li, Anthony
0aaa4f10-7283-4d03-9b1b-4a1406227349
Farrar, Nicholas R.
fabf820c-00eb-4085-9459-4b9736008771
Diamond, Scott M.
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Knecht, Cory S.
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Plaut, Roger D.
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Delury, Craig
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Aiken, Sean S.
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Laycock, Phillip A.
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Granger, Jeffrey F.
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Stoodley, Paul
08614665-92a9-4466-806e-20c6daeb483f
Dusane, Devendra H., Brooks, Jacob R., Sindeldecker, Devin, Peters, Casey W., Li, Anthony, Farrar, Nicholas R., Diamond, Scott M., Knecht, Cory S., Plaut, Roger D., Delury, Craig, Aiken, Sean S., Laycock, Phillip A., Granger, Jeffrey F. and Stoodley, Paul
(2019)
Complete killing of agar lawn biofilms by systematic spacing of antibiotic-loaded calcium sulfate beads.
Materials, 12 (24), , [4052].
(doi:10.3390/ma12244052).
Abstract
Background: Pseudomonas aeruginosa (PA) and Staphylococcus aureus (SA) are the major causative agents of acute and chronic infections. Antibiotic-loaded calcium sulfate beads (ALCSB) are used in the management of musculoskeletal infections such as periprosthetic joint infections (PJI).
Methods: To determine whether the number and spatial distribution are important factors to totally eradicate biofilms, ALCSBs containing vancomycin and tobramycin were placed on 24h agar lawn biofilms as a single bead in the center, or as 16 beads placed as four clusters of four, a ring around the edge and as a group in the center or 19 beads evenly across the plate. Bioluminescence was used to assess spatial metabolic activity in real time. Replica plating was used to assess viability.
Results: For both strains antibiotics released from the beads completely killed biofilm bacteria in a zone immediately adjacent to each bead. However, for PA extended incubation revealed the emergence of resistant colony phenotypes between the zone of eradication and the background lawn. The rate of biofilm clearing was greater when the beads were distributed evenly over the plate.
Conclusions: Both number and distribution pattern of ALCSB are important to ensure adequate coverage of antibiotics required to eradicate biofilms.
Text
materials-641538-Modified version-Final
- Accepted Manuscript
Text
materials-12-04052-v2
- Version of Record
More information
Accepted/In Press date: 29 November 2019
e-pub ahead of print date: 5 December 2019
Published date: 2019
Keywords:
antibiotic tolerance, biofilm, antibiotic-loaded bone cement, Pseudomonas, Staphylococcus, persister, periprosthetic joint infection
Identifiers
Local EPrints ID: 436315
URI: http://eprints.soton.ac.uk/id/eprint/436315
PURE UUID: f9af5d97-0f7a-42f9-95b4-a31238fc64ff
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Date deposited: 06 Dec 2019 17:30
Last modified: 17 Mar 2024 03:18
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Contributors
Author:
Devendra H. Dusane
Author:
Jacob R. Brooks
Author:
Devin Sindeldecker
Author:
Casey W. Peters
Author:
Anthony Li
Author:
Nicholas R. Farrar
Author:
Scott M. Diamond
Author:
Cory S. Knecht
Author:
Roger D. Plaut
Author:
Craig Delury
Author:
Sean S. Aiken
Author:
Phillip A. Laycock
Author:
Jeffrey F. Granger
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