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Diurnal rhythm of plasma EPA and DHA in healthy adults

Diurnal rhythm of plasma EPA and DHA in healthy adults
Diurnal rhythm of plasma EPA and DHA in healthy adults

Knowledge of the diurnal variation in circulating omega-3 polyunsaturated fatty acids (n-3 PUFAs) may be an important consideration for the development of dosing protocols designed to optimise tissue delivery of these fatty acids. The objective of the current study was to examine the variation in plasma concentrations of eicosapentaenoic acid (EPA; 20:5n-3) and docosahexaenoic acid (DHA; 22:6n-3) over a 24-h period in healthy adults under eating and sleeping conditions generally approximate to a free-living environment. Twenty-one healthy participants aged 25–44 years took part in a single laboratory visit encompassing an overnight stay. EPA and DHA were measured in plasma samples collected every two hours from 22:00 until 22:00 the following day, with all meals being provided at conventional times. Cosinor analysis was used to estimate the diurnal variation in each fatty acid from pooled data across all participants. A significant diurnal variation in the pooled plasma concentrations of both fatty acids was detected. However, evidence of distinct rhythmicity was strongest for DHA. The timing of the peak concentration of DHA was 17:43 with a corresponding nadir at 05:43. In comparison, the observed acrophase for EPA was delayed by three hours, occurring at 20:41, with a nadir at 08:41. This is the first time that the diurnal variation in these important bioactive fatty acids has been described in a sample of healthy adults following a normal pattern of eating and sleeping. In the absence of any dietary intake of EPA and DHA, circulating levels of these fatty acids fall during the overnight period and reach their lowest point in the morning. Consumption of n-3 PUFAs at night time, which counteracts this pattern, may have functional significance.

Diurnal rhythm, Docosahexaenoic acid, Eicosapentaenoic acid, Healthy population
0952-3278
1-6
Jackson, Philippa A.
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Husberg, Cathrine
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Hustvedt, Svein-Olaf
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Calder, Philip
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Khan, Julie
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Avery, Hannah
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Forster, Joanne
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Kennedy, David O.
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Jackson, Philippa A.
ba00c879-4f18-4910-a2f1-209f5f7fc2ea
Husberg, Cathrine
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Hustvedt, Svein-Olaf
ae7c50bd-137d-4768-8ab7-c47d50160784
Calder, Philip
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Khan, Julie
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Avery, Hannah
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Forster, Joanne
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Kennedy, David O.
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Jackson, Philippa A., Husberg, Cathrine, Hustvedt, Svein-Olaf, Calder, Philip, Khan, Julie, Avery, Hannah, Forster, Joanne and Kennedy, David O. (2020) Diurnal rhythm of plasma EPA and DHA in healthy adults. Prostaglandins, Leukotrienes and Essential Fatty Acids, 154, 1-6, [102054]. (doi:10.1016/j.plefa.2020.102054).

Record type: Article

Abstract

Knowledge of the diurnal variation in circulating omega-3 polyunsaturated fatty acids (n-3 PUFAs) may be an important consideration for the development of dosing protocols designed to optimise tissue delivery of these fatty acids. The objective of the current study was to examine the variation in plasma concentrations of eicosapentaenoic acid (EPA; 20:5n-3) and docosahexaenoic acid (DHA; 22:6n-3) over a 24-h period in healthy adults under eating and sleeping conditions generally approximate to a free-living environment. Twenty-one healthy participants aged 25–44 years took part in a single laboratory visit encompassing an overnight stay. EPA and DHA were measured in plasma samples collected every two hours from 22:00 until 22:00 the following day, with all meals being provided at conventional times. Cosinor analysis was used to estimate the diurnal variation in each fatty acid from pooled data across all participants. A significant diurnal variation in the pooled plasma concentrations of both fatty acids was detected. However, evidence of distinct rhythmicity was strongest for DHA. The timing of the peak concentration of DHA was 17:43 with a corresponding nadir at 05:43. In comparison, the observed acrophase for EPA was delayed by three hours, occurring at 20:41, with a nadir at 08:41. This is the first time that the diurnal variation in these important bioactive fatty acids has been described in a sample of healthy adults following a normal pattern of eating and sleeping. In the absence of any dietary intake of EPA and DHA, circulating levels of these fatty acids fall during the overnight period and reach their lowest point in the morning. Consumption of n-3 PUFAs at night time, which counteracts this pattern, may have functional significance.

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191218 DHA EPA diurnal variation - Accepted Manuscript
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More information

Accepted/In Press date: 13 January 2020
e-pub ahead of print date: 15 January 2020
Published date: March 2020
Additional Information: Copyright © 2020 The Authors. Published by Elsevier Ltd.. All rights reserved.
Keywords: Diurnal rhythm, Docosahexaenoic acid, Eicosapentaenoic acid, Healthy population

Identifiers

Local EPrints ID: 437185
URI: http://eprints.soton.ac.uk/id/eprint/437185
ISSN: 0952-3278
PURE UUID: 87f2c871-bd25-4bf1-9c1d-0a9ec703fb73
ORCID for Philip Calder: ORCID iD orcid.org/0000-0002-6038-710X

Catalogue record

Date deposited: 21 Jan 2020 17:32
Last modified: 26 Nov 2021 05:35

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Contributors

Author: Philippa A. Jackson
Author: Cathrine Husberg
Author: Svein-Olaf Hustvedt
Author: Philip Calder ORCID iD
Author: Julie Khan
Author: Hannah Avery
Author: Joanne Forster
Author: David O. Kennedy

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