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Laser-patterned paper-based sensors for rapid point-of-care detection and antibiotic-resistance testing of bacterial infections

Laser-patterned paper-based sensors for rapid point-of-care detection and antibiotic-resistance testing of bacterial infections
Laser-patterned paper-based sensors for rapid point-of-care detection and antibiotic-resistance testing of bacterial infections

Antimicrobial resistance (AMR) has been identified by the World Health Organisation as a global threat that currently claims at least 25,000 deaths each year in Europe and 700,000 globally; the number is projected to reach 10 million per year between 2015 and 2050. Therefore, there is an urgent need for low-cost but reliable point-of-care diagnostics for early screening of infections especially in developing countries lacking in basic infrastructure and trained personnel. This work is aimed at developing such a device, a paper-based microfluidic device for infection testing by an unskilled user in a low resource setting. Here, we present our work relating to the use of our laser-patterned paper-based devices for detection and susceptibility testing of Escherichia coli, via a simple visually observable colour change. The results indicate the suitability of our integrated paper devices for timely identification of bacterial infections at the point-of-care and their usefulness in providing a hugely beneficial pathway for accurate antibiotic prescribing and thus a novel route to tackling the global challenge of AMR.

Antimicrobial resistance, Escherichia coli, Point-of-care, Bacterial pathogen detection, Antibiotic-resistance testing, Paper-based device
0956-5663
He, Peijun J.W.
2e303166-6aa5-4a09-b22e-440d96a54a9f
Katis, Ioannis N.
f92dfb8f-610d-4877-83f6-fd26a571df12
Kumar, Anto J.U.
202a7bb5-ef3b-4049-8749-3e4b91418c78
Bryant, Catherine A.
d53ab6c9-909d-43cb-84fc-3e197df377f3
Keevil, Charles W.
cb7de0a7-ce33-4cfa-af52-07f99e5650eb
Somani, Bhaskar K.
ab5fd1ce-02df-4b88-b25e-8ece396335d9
Mahobia, Nitin
cf61df75-633e-4622-abc7-271979c13d3f
Eason, Robert W.
e38684c3-d18c-41b9-a4aa-def67283b020
Sones, Collin L.
9de9d8ee-d394-46a5-80b7-e341c0eed0a8
He, Peijun J.W.
2e303166-6aa5-4a09-b22e-440d96a54a9f
Katis, Ioannis N.
f92dfb8f-610d-4877-83f6-fd26a571df12
Kumar, Anto J.U.
202a7bb5-ef3b-4049-8749-3e4b91418c78
Bryant, Catherine A.
d53ab6c9-909d-43cb-84fc-3e197df377f3
Keevil, Charles W.
cb7de0a7-ce33-4cfa-af52-07f99e5650eb
Somani, Bhaskar K.
ab5fd1ce-02df-4b88-b25e-8ece396335d9
Mahobia, Nitin
cf61df75-633e-4622-abc7-271979c13d3f
Eason, Robert W.
e38684c3-d18c-41b9-a4aa-def67283b020
Sones, Collin L.
9de9d8ee-d394-46a5-80b7-e341c0eed0a8

He, Peijun J.W., Katis, Ioannis N., Kumar, Anto J.U., Bryant, Catherine A., Keevil, Charles W., Somani, Bhaskar K., Mahobia, Nitin, Eason, Robert W. and Sones, Collin L. (2020) Laser-patterned paper-based sensors for rapid point-of-care detection and antibiotic-resistance testing of bacterial infections. Biosensors & Bioelectronics, 152, [112008]. (doi:10.1016/j.bios.2020.112008).

Record type: Article

Abstract

Antimicrobial resistance (AMR) has been identified by the World Health Organisation as a global threat that currently claims at least 25,000 deaths each year in Europe and 700,000 globally; the number is projected to reach 10 million per year between 2015 and 2050. Therefore, there is an urgent need for low-cost but reliable point-of-care diagnostics for early screening of infections especially in developing countries lacking in basic infrastructure and trained personnel. This work is aimed at developing such a device, a paper-based microfluidic device for infection testing by an unskilled user in a low resource setting. Here, we present our work relating to the use of our laser-patterned paper-based devices for detection and susceptibility testing of Escherichia coli, via a simple visually observable colour change. The results indicate the suitability of our integrated paper devices for timely identification of bacterial infections at the point-of-care and their usefulness in providing a hugely beneficial pathway for accurate antibiotic prescribing and thus a novel route to tackling the global challenge of AMR.

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Manuscript_final - Accepted Manuscript
Restricted to Repository staff only until 9 January 2021.
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More information

Accepted/In Press date: 6 January 2020
e-pub ahead of print date: 9 January 2020
Published date: 15 March 2020
Keywords: Antimicrobial resistance, Escherichia coli, Point-of-care, Bacterial pathogen detection, Antibiotic-resistance testing, Paper-based device

Identifiers

Local EPrints ID: 437779
URI: http://eprints.soton.ac.uk/id/eprint/437779
ISSN: 0956-5663
PURE UUID: 680a4d0a-9504-411f-9817-6ffeb9ec7ed5
ORCID for Ioannis N. Katis: ORCID iD orcid.org/0000-0002-2016-557X
ORCID for Charles W. Keevil: ORCID iD orcid.org/0000-0003-1917-7706
ORCID for Robert W. Eason: ORCID iD orcid.org/0000-0001-9704-2204

Catalogue record

Date deposited: 17 Feb 2020 17:30
Last modified: 13 May 2020 00:41

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