Multiscale three-dimensional imaging of the placenta
Multiscale three-dimensional imaging of the placenta
Placental function involves multiple different processes which operate at different scales from centimetres to nanometres. Everything that the placenta does from mediating blood flow to gene expression, occurs within a three-dimensional anatomical framework. This review outlines how multiscale three-dimensional imaging approaches can provide insight into placental structure and function. Three-dimensional imaging approaches include microCT, confocal, super resolution, light-sheet, and serial block-face scanning electron microscopy. Used together, these approaches allow three-dimensional imaging of the placenta across the scales at which different processes occur. Three-dimensional imaging illustrates the spatial relationships between structures and visualises structures that are not clearly apparent in two-dimensions. Understanding the three-dimensional structure of the placenta enables exploration of the relationship between structure and function, including through the development of computational models based on realistic geometries. Three-dimensional imaging approaches will enhance our understanding of placental function in health and disease.
Confocal, Imaging, Light-sheet microscopy, Placenta, Serial block face scanning electron microscopy, microCt
55-60
Lewis, Rohan M.
caaeb97d-ea69-4f7b-8adb-5fa25e2d3502
Pearson-Farr, Jennifer E.
e7da2e7c-e8e8-47d6-aca4-86b1a5b90653
December 2020
Lewis, Rohan M.
caaeb97d-ea69-4f7b-8adb-5fa25e2d3502
Pearson-Farr, Jennifer E.
e7da2e7c-e8e8-47d6-aca4-86b1a5b90653
Abstract
Placental function involves multiple different processes which operate at different scales from centimetres to nanometres. Everything that the placenta does from mediating blood flow to gene expression, occurs within a three-dimensional anatomical framework. This review outlines how multiscale three-dimensional imaging approaches can provide insight into placental structure and function. Three-dimensional imaging approaches include microCT, confocal, super resolution, light-sheet, and serial block-face scanning electron microscopy. Used together, these approaches allow three-dimensional imaging of the placenta across the scales at which different processes occur. Three-dimensional imaging illustrates the spatial relationships between structures and visualises structures that are not clearly apparent in two-dimensions. Understanding the three-dimensional structure of the placenta enables exploration of the relationship between structure and function, including through the development of computational models based on realistic geometries. Three-dimensional imaging approaches will enhance our understanding of placental function in health and disease.
Text
Multiscale imaging review for resubmission
- Accepted Manuscript
More information
Accepted/In Press date: 31 January 2020
e-pub ahead of print date: 1 February 2020
Published date: December 2020
Additional Information:
Funding Information:
RL is funded by the Leverhulme Trust ( RPG-2019-208 ). Images were produced with support of the Biomedical Imaging Unit and muVIS X-ray imaging centre . The authors would like to thank Victoria Isakzai for producing images in Fig. 3 .
Publisher Copyright:
© 2020 Elsevier Ltd
Keywords:
Confocal, Imaging, Light-sheet microscopy, Placenta, Serial block face scanning electron microscopy, microCt
Identifiers
Local EPrints ID: 438232
URI: http://eprints.soton.ac.uk/id/eprint/438232
ISSN: 0143-4004
PURE UUID: 07f0b594-89b3-46a7-9f32-581a474844d3
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Date deposited: 04 Mar 2020 17:31
Last modified: 17 Mar 2024 05:22
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Author:
Jennifer E. Pearson-Farr
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