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Differential postprandial incorporation of 20:5n-3 and 22:6n-3 into individual plasma triacylglycerol and phosphatidylcholine molecular species in humans

Differential postprandial incorporation of 20:5n-3 and 22:6n-3 into individual plasma triacylglycerol and phosphatidylcholine molecular species in humans
Differential postprandial incorporation of 20:5n-3 and 22:6n-3 into individual plasma triacylglycerol and phosphatidylcholine molecular species in humans
The mechanisms by which digested fat is absorbed and transported in the circulation are well documented. However, it is uncertain whether the molecular species composition of dietary fats influences the molecular species composition of meal-derived lipids in blood. This may be important because enzymes that remove meal-derived fatty acids from the circulation exhibit differential activities towards individual lipid molecular species. To determine the effect of consuming oils with different molecular compositions on the incorporation of 20:5n-3 and 22:6n-3 into plasma lipid molecular species. Men and women (18 - 30 years) consumed standardised meals containing 20:5n-5 and 22:6n-3 (total 450mg) provided by an oil from transgenic Camelina sativa (CSO) or a blended fish oil (BFO) which differed in the composition of 20:5n-3 and 22:6n-3 – containing molecular species. Blood was collected during the subsequent 8 hours. Samples were analysed by liquid chromatography-mass spectrometry. The molecular species composition of the test oils was distinct from the composition of plasma triacylglycerol (TG) or phosphatidylcholine (PC) molecular species at baseline and at 1.5 or 6 hours after the meal. The rank order by concentration of both plasma PC and TG molecular species at baseline was maintained during the postprandial period. 20:5n-3 and 22:6n-3 were incorporated preferentially into plasma PC compared to plasma TG. Together these findings suggest that the composition of dietary lipids undergoes extensive rearrangement after absorption, such that plasma TG and PC maintain their molecular species composition, which may facilitate lipase activities in blood and/or influence lipoprotein structural stability and function.
Docosahexaenoic acid, Phosphatidylcholine, eicosapentaenoic acid, molecular species, postprandial, triacylglycerol
1388-1981
West, A
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Michaelson, Louise
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Miles, Elizabeth
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Haslam, Richard
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Lillycrop, Karen
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Georgescu, Ramona
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Han, Lihua
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Sayanova, Olga
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Napier, Johnathan
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Calder, Philip
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Burdge, Graham
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West, A
684d348c-563d-4818-a4c0-fe4834cc038b
Michaelson, Louise
c0b5d276-6ab0-43b2-a798-cbddce5e36a4
Miles, Elizabeth
20332899-ecdb-4214-95bc-922dde36d416
Haslam, Richard
9bc4a834-54b5-4773-b891-12bf009a139e
Lillycrop, Karen
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Georgescu, Ramona
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Han, Lihua
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Sayanova, Olga
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Napier, Johnathan
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Calder, Philip
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Burdge, Graham
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West, A, Michaelson, Louise, Miles, Elizabeth, Haslam, Richard, Lillycrop, Karen, Georgescu, Ramona, Han, Lihua, Sayanova, Olga, Napier, Johnathan, Calder, Philip and Burdge, Graham (2020) Differential postprandial incorporation of 20:5n-3 and 22:6n-3 into individual plasma triacylglycerol and phosphatidylcholine molecular species in humans. Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids, 1865 (8), [158710]. (doi:10.1016/j.bbalip.2020.158710).

Record type: Article

Abstract

The mechanisms by which digested fat is absorbed and transported in the circulation are well documented. However, it is uncertain whether the molecular species composition of dietary fats influences the molecular species composition of meal-derived lipids in blood. This may be important because enzymes that remove meal-derived fatty acids from the circulation exhibit differential activities towards individual lipid molecular species. To determine the effect of consuming oils with different molecular compositions on the incorporation of 20:5n-3 and 22:6n-3 into plasma lipid molecular species. Men and women (18 - 30 years) consumed standardised meals containing 20:5n-5 and 22:6n-3 (total 450mg) provided by an oil from transgenic Camelina sativa (CSO) or a blended fish oil (BFO) which differed in the composition of 20:5n-3 and 22:6n-3 – containing molecular species. Blood was collected during the subsequent 8 hours. Samples were analysed by liquid chromatography-mass spectrometry. The molecular species composition of the test oils was distinct from the composition of plasma triacylglycerol (TG) or phosphatidylcholine (PC) molecular species at baseline and at 1.5 or 6 hours after the meal. The rank order by concentration of both plasma PC and TG molecular species at baseline was maintained during the postprandial period. 20:5n-3 and 22:6n-3 were incorporated preferentially into plasma PC compared to plasma TG. Together these findings suggest that the composition of dietary lipids undergoes extensive rearrangement after absorption, such that plasma TG and PC maintain their molecular species composition, which may facilitate lipase activities in blood and/or influence lipoprotein structural stability and function.

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West Accepted version 30 March 2020 - Accepted Manuscript
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Accepted/In Press date: 6 April 2020
e-pub ahead of print date: 11 April 2020
Published date: August 2020
Keywords: Docosahexaenoic acid, Phosphatidylcholine, eicosapentaenoic acid, molecular species, postprandial, triacylglycerol

Identifiers

Local EPrints ID: 439203
URI: http://eprints.soton.ac.uk/id/eprint/439203
ISSN: 1388-1981
PURE UUID: 9367a887-81be-4d12-9f38-52c677e871a3
ORCID for Elizabeth Miles: ORCID iD orcid.org/0000-0002-8643-0655
ORCID for Karen Lillycrop: ORCID iD orcid.org/0000-0001-7350-5489
ORCID for Philip Calder: ORCID iD orcid.org/0000-0002-6038-710X
ORCID for Graham Burdge: ORCID iD orcid.org/0000-0002-7665-2967

Catalogue record

Date deposited: 07 Apr 2020 16:30
Last modified: 26 Nov 2021 05:37

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Contributors

Author: A West
Author: Louise Michaelson
Author: Elizabeth Miles ORCID iD
Author: Richard Haslam
Author: Karen Lillycrop ORCID iD
Author: Ramona Georgescu
Author: Lihua Han
Author: Olga Sayanova
Author: Johnathan Napier
Author: Philip Calder ORCID iD
Author: Graham Burdge ORCID iD

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