Differential postprandial incorporation of 20:5n-3 and 22:6n-3 into individual plasma triacylglycerol and phosphatidylcholine molecular species in humans
Differential postprandial incorporation of 20:5n-3 and 22:6n-3 into individual plasma triacylglycerol and phosphatidylcholine molecular species in humans
The mechanisms by which digested fat is absorbed and transported in the circulation are well documented. However, it is uncertain whether the molecular species composition of dietary fats influences the molecular species composition of meal-derived lipids in blood. This may be important because enzymes that remove meal-derived fatty acids from the circulation exhibit differential activities towards individual lipid molecular species. To determine the effect of consuming oils with different molecular compositions on the incorporation of 20:5n-3 and 22:6n-3 into plasma lipid molecular species. Men and women (18 - 30 years) consumed standardised meals containing 20:5n-5 and 22:6n-3 (total 450mg) provided by an oil from transgenic Camelina sativa (CSO) or a blended fish oil (BFO) which differed in the composition of 20:5n-3 and 22:6n-3 – containing molecular species. Blood was collected during the subsequent 8 hours. Samples were analysed by liquid chromatography-mass spectrometry. The molecular species composition of the test oils was distinct from the composition of plasma triacylglycerol (TG) or phosphatidylcholine (PC) molecular species at baseline and at 1.5 or 6 hours after the meal. The rank order by concentration of both plasma PC and TG molecular species at baseline was maintained during the postprandial period. 20:5n-3 and 22:6n-3 were incorporated preferentially into plasma PC compared to plasma TG. Together these findings suggest that the composition of dietary lipids undergoes extensive rearrangement after absorption, such that plasma TG and PC maintain their molecular species composition, which may facilitate lipase activities in blood and/or influence lipoprotein structural stability and function.
Docosahexaenoic acid, Phosphatidylcholine, eicosapentaenoic acid, molecular species, postprandial, triacylglycerol
West, A
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Michaelson, Louise
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Miles, Elizabeth
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Haslam, Richard
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Lillycrop, Karen
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Georgescu, Ramona
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Han, Lihua
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Sayanova, Olga
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Napier, Johnathan
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Calder, Philip
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Burdge, Graham
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August 2020
West, A
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Michaelson, Louise
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Miles, Elizabeth
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Haslam, Richard
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Lillycrop, Karen
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Georgescu, Ramona
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Han, Lihua
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Sayanova, Olga
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Napier, Johnathan
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Calder, Philip
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Burdge, Graham
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West, A, Michaelson, Louise, Miles, Elizabeth, Haslam, Richard, Lillycrop, Karen, Georgescu, Ramona, Han, Lihua, Sayanova, Olga, Napier, Johnathan, Calder, Philip and Burdge, Graham
(2020)
Differential postprandial incorporation of 20:5n-3 and 22:6n-3 into individual plasma triacylglycerol and phosphatidylcholine molecular species in humans.
Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids, 1865 (8), [158710].
(doi:10.1016/j.bbalip.2020.158710).
Abstract
The mechanisms by which digested fat is absorbed and transported in the circulation are well documented. However, it is uncertain whether the molecular species composition of dietary fats influences the molecular species composition of meal-derived lipids in blood. This may be important because enzymes that remove meal-derived fatty acids from the circulation exhibit differential activities towards individual lipid molecular species. To determine the effect of consuming oils with different molecular compositions on the incorporation of 20:5n-3 and 22:6n-3 into plasma lipid molecular species. Men and women (18 - 30 years) consumed standardised meals containing 20:5n-5 and 22:6n-3 (total 450mg) provided by an oil from transgenic Camelina sativa (CSO) or a blended fish oil (BFO) which differed in the composition of 20:5n-3 and 22:6n-3 – containing molecular species. Blood was collected during the subsequent 8 hours. Samples were analysed by liquid chromatography-mass spectrometry. The molecular species composition of the test oils was distinct from the composition of plasma triacylglycerol (TG) or phosphatidylcholine (PC) molecular species at baseline and at 1.5 or 6 hours after the meal. The rank order by concentration of both plasma PC and TG molecular species at baseline was maintained during the postprandial period. 20:5n-3 and 22:6n-3 were incorporated preferentially into plasma PC compared to plasma TG. Together these findings suggest that the composition of dietary lipids undergoes extensive rearrangement after absorption, such that plasma TG and PC maintain their molecular species composition, which may facilitate lipase activities in blood and/or influence lipoprotein structural stability and function.
Text
West Accepted version 30 March 2020
- Accepted Manuscript
More information
Accepted/In Press date: 6 April 2020
e-pub ahead of print date: 11 April 2020
Published date: August 2020
Additional Information:
Funding Information:
This work was supported by grants from the Biotechnology and Biological Sciences Research Council (BBSRC) BB/N014081/1 and BB/N01412X/1 . LVM and RPH are supported by the BBSRC Institute Strategic Programme Tailoring Plant Metabolism BBS/E/C/000I0420 . The funder was not involved in the collection, analysis or interpretation of data; in the writing of the report; nor in the decision to submit the article for publication.
Funding Information:
GCB has received research funding from Nestle, Abbott Nutrition and Danone. He has served as member of the Scientific Advisory Board of BASF and is member of the BASF Asia Grant Panel. PCC acts as a consultant to BASF AS, Smartfish, DSM, Danone and Fresenius-Kabi. JAN has provided ad hoc consultancy services to BASF. The other authors state they have nothing to declare.
Publisher Copyright:
© 2020 Elsevier B.V.
Keywords:
Docosahexaenoic acid, Phosphatidylcholine, eicosapentaenoic acid, molecular species, postprandial, triacylglycerol
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Local EPrints ID: 439203
URI: http://eprints.soton.ac.uk/id/eprint/439203
ISSN: 1388-1981
PURE UUID: 9367a887-81be-4d12-9f38-52c677e871a3
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Date deposited: 07 Apr 2020 16:30
Last modified: 17 Mar 2024 05:28
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Contributors
Author:
A West
Author:
Louise Michaelson
Author:
Richard Haslam
Author:
Ramona Georgescu
Author:
Lihua Han
Author:
Olga Sayanova
Author:
Johnathan Napier
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