The University of Southampton
University of Southampton Institutional Repository
Warning ePrints Soton is experiencing an issue with some file downloads not being available. We are working hard to fix this. Please bear with us.

Genome-wide DNA methylation in peripheral blood, and long-term exposure to source-specific transportation noise and air pollution, the SAPALDIA study

Genome-wide DNA methylation in peripheral blood, and long-term exposure to source-specific transportation noise and air pollution, the SAPALDIA study
Genome-wide DNA methylation in peripheral blood, and long-term exposure to source-specific transportation noise and air pollution, the SAPALDIA study
Background:
Few epigenome-wide association studies (EWAS) on air pollutants exist, and none have been done on transportation noise exposures, which also contribute to environmental burden of disease.

Objective:
We performed mutually independent EWAS on transportation noise and air pollution exposures.

Methods:
We used data from two time points of the Swiss Cohort Study on Air Pollution and Lung and Heart Diseases in Adults (SAPALDIA) from 1,389 participants contributing 2,542 observations. We applied multiexposure linear mixed-effects regressions with participant-level random intercept to identify significant Cytosine-phosphate-Guanine (CpG) sites and differentially methylated regions (DMRs) in relation to 1-y average aircraft, railway, and road traffic day-evening-night noise (Lden); nitrogen dioxide (NO2); and particulate matter (PM) with aerodynamic diameter <2.5μm (PM2.5). We performed candidate (CpG-based; cross-systemic phenotypes, combined into “allostatic load”) and agnostic (DMR-based) pathway enrichment tests, and replicated previously reported air pollution EWAS signals.

Results:
We found no statistically significant CpGs at false discovery rate <0.05. However, 14, 48, 183, 8, and 71 DMRs independently associated with aircraft, railway, and road traffic Lden; NO2; and PM2.5, respectively, with minimally overlapping signals. Transportation Lden and air pollutants tendentially associated with decreased and increased methylation, respectively. We observed significant enrichment of candidate DNA methylation related to C-reactive protein and body mass index (aircraft, road traffic Lden, and PM2.5), renal function and “allostatic load” (all exposures). Agnostic functional networks related to cellular immunity, gene expression, cell growth/proliferation, cardiovascular, auditory, embryonic, and neurological systems development were enriched. We replicated increased methylation in cg08500171 (NO2) and decreased methylation in cg17629796 (PM2.5).

Conclusions:
Mutually independent DNA methylation was associated with source-specific transportation noise and air pollution exposures, with distinct and shared enrichments for pathways related to inflammation, cellular development, and immune responses. These findings contribute in clarifying the pathways linking these exposures and age-related diseases but need further confirmation in the context of mediation analyses. https://doi.org/10.1289/EHP6174
0091-6765
1-14
Eze, Ikenna C.
e29f8a9c-8206-478a-b12c-23c8d28df77b
Jeong, Ayoung
d742adca-f0fd-4da7-89ba-a01b5e879664
Schaffner, Emmanuel
293d2f9d-6359-4e3f-93ce-b8c052c888a3
Rezwan, Faisal I.
203f8f38-1f5d-485b-ab11-c546b4276338
Ghantous, Akram
c078d3dc-5baa-4ec3-b157-f200f843a0d1
Foraster, Maria
8968447a-d909-49c0-a62a-ac37104b1770
Vienneau, Danielle
a56c1424-c49c-49d0-a898-69c94b28eab8
Kronenberg, Florian
056e8fa9-c2f1-4d61-a849-19ad35ed5c34
Herceg, Zdenko
489f5d2b-bf53-4ddd-89fc-68ec41b5d528
Vineis, Paolo
8ea44c7a-2278-4a1b-a68a-8c879b0f59d7
Brink, Mark
0a2d35ea-7697-4797-ab54-58373c9becd6
Wunderli, Jean-Marc
909e555a-0658-4203-91aa-2524fa245976
Schindler, Christian
c17229fe-9c92-46ff-9d28-13b93219d814
Cajochen, Christian
f605e720-e417-45dc-9b5c-244b1a1d6265
Roosli, Martin
8886efbe-1275-422a-adee-846b24759d41
Holloway, John
4bbd77e6-c095-445d-a36b-a50a72f6fe1a
Imboden, Medea
7ac4495d-e803-4e18-b6ba-318da568f776
Probst-Hensch, Nicole
33ee3cca-a1d4-4157-87c3-20ebfd84406f
Eze, Ikenna C.
e29f8a9c-8206-478a-b12c-23c8d28df77b
Jeong, Ayoung
d742adca-f0fd-4da7-89ba-a01b5e879664
Schaffner, Emmanuel
293d2f9d-6359-4e3f-93ce-b8c052c888a3
Rezwan, Faisal I.
203f8f38-1f5d-485b-ab11-c546b4276338
Ghantous, Akram
c078d3dc-5baa-4ec3-b157-f200f843a0d1
Foraster, Maria
8968447a-d909-49c0-a62a-ac37104b1770
Vienneau, Danielle
a56c1424-c49c-49d0-a898-69c94b28eab8
Kronenberg, Florian
056e8fa9-c2f1-4d61-a849-19ad35ed5c34
Herceg, Zdenko
489f5d2b-bf53-4ddd-89fc-68ec41b5d528
Vineis, Paolo
8ea44c7a-2278-4a1b-a68a-8c879b0f59d7
Brink, Mark
0a2d35ea-7697-4797-ab54-58373c9becd6
Wunderli, Jean-Marc
909e555a-0658-4203-91aa-2524fa245976
Schindler, Christian
c17229fe-9c92-46ff-9d28-13b93219d814
Cajochen, Christian
f605e720-e417-45dc-9b5c-244b1a1d6265
Roosli, Martin
8886efbe-1275-422a-adee-846b24759d41
Holloway, John
4bbd77e6-c095-445d-a36b-a50a72f6fe1a
Imboden, Medea
7ac4495d-e803-4e18-b6ba-318da568f776
Probst-Hensch, Nicole
33ee3cca-a1d4-4157-87c3-20ebfd84406f

Eze, Ikenna C., Jeong, Ayoung, Schaffner, Emmanuel, Rezwan, Faisal I., Ghantous, Akram, Foraster, Maria, Vienneau, Danielle, Kronenberg, Florian, Herceg, Zdenko, Vineis, Paolo, Brink, Mark, Wunderli, Jean-Marc, Schindler, Christian, Cajochen, Christian, Roosli, Martin, Holloway, John, Imboden, Medea and Probst-Hensch, Nicole (2020) Genome-wide DNA methylation in peripheral blood, and long-term exposure to source-specific transportation noise and air pollution, the SAPALDIA study. Environmental Health Perspectives, 128 (6), 1-14, [67003]. (doi:10.1289/EHP6174).

Record type: Article

Abstract

Background:
Few epigenome-wide association studies (EWAS) on air pollutants exist, and none have been done on transportation noise exposures, which also contribute to environmental burden of disease.

Objective:
We performed mutually independent EWAS on transportation noise and air pollution exposures.

Methods:
We used data from two time points of the Swiss Cohort Study on Air Pollution and Lung and Heart Diseases in Adults (SAPALDIA) from 1,389 participants contributing 2,542 observations. We applied multiexposure linear mixed-effects regressions with participant-level random intercept to identify significant Cytosine-phosphate-Guanine (CpG) sites and differentially methylated regions (DMRs) in relation to 1-y average aircraft, railway, and road traffic day-evening-night noise (Lden); nitrogen dioxide (NO2); and particulate matter (PM) with aerodynamic diameter <2.5μm (PM2.5). We performed candidate (CpG-based; cross-systemic phenotypes, combined into “allostatic load”) and agnostic (DMR-based) pathway enrichment tests, and replicated previously reported air pollution EWAS signals.

Results:
We found no statistically significant CpGs at false discovery rate <0.05. However, 14, 48, 183, 8, and 71 DMRs independently associated with aircraft, railway, and road traffic Lden; NO2; and PM2.5, respectively, with minimally overlapping signals. Transportation Lden and air pollutants tendentially associated with decreased and increased methylation, respectively. We observed significant enrichment of candidate DNA methylation related to C-reactive protein and body mass index (aircraft, road traffic Lden, and PM2.5), renal function and “allostatic load” (all exposures). Agnostic functional networks related to cellular immunity, gene expression, cell growth/proliferation, cardiovascular, auditory, embryonic, and neurological systems development were enriched. We replicated increased methylation in cg08500171 (NO2) and decreased methylation in cg17629796 (PM2.5).

Conclusions:
Mutually independent DNA methylation was associated with source-specific transportation noise and air pollution exposures, with distinct and shared enrichments for pathways related to inflammation, cellular development, and immune responses. These findings contribute in clarifying the pathways linking these exposures and age-related diseases but need further confirmation in the context of mediation analyses. https://doi.org/10.1289/EHP6174

Text
R3 Manuscript with Tables and Figure Legend [1] - Accepted Manuscript
Download (604kB)
Text
R3 Figure 1 - Accepted Manuscript
Download (122kB)
Text
R3 Figure 2 A B - Accepted Manuscript
Download (216kB)
Text
EHP6174 - Version of Record
Available under License Other.
Download (465kB)

More information

Accepted/In Press date: 30 April 2020
e-pub ahead of print date: 1 June 2020
Published date: June 2020

Identifiers

Local EPrints ID: 440771
URI: http://eprints.soton.ac.uk/id/eprint/440771
ISSN: 0091-6765
PURE UUID: 31c0d492-ad0d-4e91-a90f-79baa217b3db
ORCID for Faisal I. Rezwan: ORCID iD orcid.org/0000-0001-9921-222X
ORCID for John Holloway: ORCID iD orcid.org/0000-0001-9998-0464

Catalogue record

Date deposited: 15 May 2020 16:57
Last modified: 26 Nov 2021 05:18

Export record

Altmetrics

Contributors

Author: Ikenna C. Eze
Author: Ayoung Jeong
Author: Emmanuel Schaffner
Author: Faisal I. Rezwan ORCID iD
Author: Akram Ghantous
Author: Maria Foraster
Author: Danielle Vienneau
Author: Florian Kronenberg
Author: Zdenko Herceg
Author: Paolo Vineis
Author: Mark Brink
Author: Jean-Marc Wunderli
Author: Christian Schindler
Author: Christian Cajochen
Author: Martin Roosli
Author: John Holloway ORCID iD
Author: Medea Imboden
Author: Nicole Probst-Hensch

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of http://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×