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Ultrastructure imaging of Pseudomonas aeruginosa lawn biofilms and eradication of the tobramycin-resistant variants under in vitro electroceutical treatment

Ultrastructure imaging of Pseudomonas aeruginosa lawn biofilms and eradication of the tobramycin-resistant variants under in vitro electroceutical treatment
Ultrastructure imaging of Pseudomonas aeruginosa lawn biofilms and eradication of the tobramycin-resistant variants under in vitro electroceutical treatment
Electrochemically generated bactericidal compounds have been shown to eradicate bacterial lawn biofilms through electroceutical treatment. However, the ultrastructure of biofilms exposed to these species has not been studied. Moreover, it is unknown if the efficacy of electroceutical treatment extends to antibiotic-resistant variants that emerge in lawn biofilms after antibiotic treatment. In this report, the efficacy of the in vitro electroceutical treatment of Pseudomonas aeruginosa biofilms is demonstrated both at room temperature and in an incubator, with a ~ 4 log decrease (p < 0.01) in the biofilm viability observed on the anode at both conditions. The ultrastructure changes in the lawn biofilms imaged using transmission electron microscopy demonstrate significant bacterial cell damage at the anode after 24 h of electroceutical treatment. A mix of both damaged and undamaged cells was observed at the cathode. Finally, both eradication and prevention of the emergence of tobramycin-resistant variants were demonstrated by combining antibiotic treatment with electroceutical treatment on the lawn biofilms.
Ultrastructure imaging, Pseudomonas aeruginosa, lawn biofilms, tobramycin-resistant variants, in vitro electroceutical treatment
2045-2322
Lochab, Varun
64ab1066-34f4-4bea-b8e9-089d0014c02c
Jones, Travis
c2e4ba02-4f53-4faf-8ca2-c5f7514cb6ae
Dusane, Devendra H.
9a47c5eb-5587-4f1d-bfd4-8548681be2bc
Peters, Casey W.
7c4253bd-aa41-42f3-a253-9da8bd3acb13
Stoodley, Paul
08614665-92a9-4466-806e-20c6daeb483f
Wozniak, Daniel J.
bfa8e8e5-5929-449b-af6a-ec2d86c47eb5
Subramaniam, Vish V.
102f9a0a-89f0-4b6d-9d1c-2556bfa00f8c
Prakash, Shaurya
54775442-9f96-4129-b93d-082a5ff91d9b
Lochab, Varun
64ab1066-34f4-4bea-b8e9-089d0014c02c
Jones, Travis
c2e4ba02-4f53-4faf-8ca2-c5f7514cb6ae
Dusane, Devendra H.
9a47c5eb-5587-4f1d-bfd4-8548681be2bc
Peters, Casey W.
7c4253bd-aa41-42f3-a253-9da8bd3acb13
Stoodley, Paul
08614665-92a9-4466-806e-20c6daeb483f
Wozniak, Daniel J.
bfa8e8e5-5929-449b-af6a-ec2d86c47eb5
Subramaniam, Vish V.
102f9a0a-89f0-4b6d-9d1c-2556bfa00f8c
Prakash, Shaurya
54775442-9f96-4129-b93d-082a5ff91d9b

Lochab, Varun, Jones, Travis, Dusane, Devendra H., Peters, Casey W., Stoodley, Paul, Wozniak, Daniel J., Subramaniam, Vish V. and Prakash, Shaurya (2020) Ultrastructure imaging of Pseudomonas aeruginosa lawn biofilms and eradication of the tobramycin-resistant variants under in vitro electroceutical treatment. Scientific Reports. (In Press)

Record type: Article

Abstract

Electrochemically generated bactericidal compounds have been shown to eradicate bacterial lawn biofilms through electroceutical treatment. However, the ultrastructure of biofilms exposed to these species has not been studied. Moreover, it is unknown if the efficacy of electroceutical treatment extends to antibiotic-resistant variants that emerge in lawn biofilms after antibiotic treatment. In this report, the efficacy of the in vitro electroceutical treatment of Pseudomonas aeruginosa biofilms is demonstrated both at room temperature and in an incubator, with a ~ 4 log decrease (p < 0.01) in the biofilm viability observed on the anode at both conditions. The ultrastructure changes in the lawn biofilms imaged using transmission electron microscopy demonstrate significant bacterial cell damage at the anode after 24 h of electroceutical treatment. A mix of both damaged and undamaged cells was observed at the cathode. Finally, both eradication and prevention of the emergence of tobramycin-resistant variants were demonstrated by combining antibiotic treatment with electroceutical treatment on the lawn biofilms.

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Accepted/In Press date: 22 May 2020
Keywords: Ultrastructure imaging, Pseudomonas aeruginosa, lawn biofilms, tobramycin-resistant variants, in vitro electroceutical treatment

Identifiers

Local EPrints ID: 441114
URI: http://eprints.soton.ac.uk/id/eprint/441114
ISSN: 2045-2322
PURE UUID: 4366ebf4-7b09-4fb7-a5be-739c2944ab71
ORCID for Paul Stoodley: ORCID iD orcid.org/0000-0001-6069-273X

Catalogue record

Date deposited: 02 Jun 2020 16:30
Last modified: 13 Dec 2021 06:02

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Contributors

Author: Varun Lochab
Author: Travis Jones
Author: Devendra H. Dusane
Author: Casey W. Peters
Author: Paul Stoodley ORCID iD
Author: Daniel J. Wozniak
Author: Vish V. Subramaniam
Author: Shaurya Prakash

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