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Correlative fluorescence and atomic force microscopy to advance the bio-physical characterisation of co-culture of living cells

Correlative fluorescence and atomic force microscopy to advance the bio-physical characterisation of co-culture of living cells
Correlative fluorescence and atomic force microscopy to advance the bio-physical characterisation of co-culture of living cells
An understanding of the cell mechanical properties involved in numerous cellular processes including cell division, cell migration/invasion, and cell morphology, is crucial in developing and informing cell physiology and function. Atomic force microscopy (AFM) offers a powerful biophysical technique that facilitates the imaging of living cells under physiological buffer conditions. However, AFM in isolation cannot discriminate between different cell types within heterogeneous samples for example in a solid biopsy. The current studies demonstrate the potential of AFM in combination with correlative fluorescence optical sectioning microscopy for live cell imaging. Furthermore, this work establishes the advantage of fluorescence-AFM imaging to distinguish and analyse single-cell bio-physical properties in mixed human cell populations, in real-time. Critically, our results show that correlative fluorescence-AFM imaging allows the simultaneous co-localised detection of fluorescence coupled with nano-mechanical mapping. The findings from this work contribute to the promotion and dissemination of correlative multimodal imaging in life sciences, providing a platform for further investigations in biological and pre-clinical research.
0006-291X
392-397
Moura, C.
3dd7eb32-f5e7-44dd-9b40-6eba803261fb
Miranda, A
2da17b7c-ecf3-4d5f-a2ef-29f550e5df84
Oreffo, Richard
ff9fff72-6855-4d0f-bfb2-311d0e8f3778
De Beule, Pieter
9ba69332-b4c0-4948-a11f-2010664222b7
Moura, C.
3dd7eb32-f5e7-44dd-9b40-6eba803261fb
Miranda, A
2da17b7c-ecf3-4d5f-a2ef-29f550e5df84
Oreffo, Richard
ff9fff72-6855-4d0f-bfb2-311d0e8f3778
De Beule, Pieter
9ba69332-b4c0-4948-a11f-2010664222b7

Moura, C., Miranda, A, Oreffo, Richard and De Beule, Pieter (2020) Correlative fluorescence and atomic force microscopy to advance the bio-physical characterisation of co-culture of living cells. Biochemical and Biophysical Research Communications, 529 (2), 392-397. (doi:10.1016/j.bbrc.2020.06.037).

Record type: Article

Abstract

An understanding of the cell mechanical properties involved in numerous cellular processes including cell division, cell migration/invasion, and cell morphology, is crucial in developing and informing cell physiology and function. Atomic force microscopy (AFM) offers a powerful biophysical technique that facilitates the imaging of living cells under physiological buffer conditions. However, AFM in isolation cannot discriminate between different cell types within heterogeneous samples for example in a solid biopsy. The current studies demonstrate the potential of AFM in combination with correlative fluorescence optical sectioning microscopy for live cell imaging. Furthermore, this work establishes the advantage of fluorescence-AFM imaging to distinguish and analyse single-cell bio-physical properties in mixed human cell populations, in real-time. Critically, our results show that correlative fluorescence-AFM imaging allows the simultaneous co-localised detection of fluorescence coupled with nano-mechanical mapping. The findings from this work contribute to the promotion and dissemination of correlative multimodal imaging in life sciences, providing a platform for further investigations in biological and pre-clinical research.

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Manuscript-CostaMoura2020 - Accepted Manuscript
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More information

Accepted/In Press date: 8 June 2020
e-pub ahead of print date: 1 July 2020
Published date: 20 August 2020

Identifiers

Local EPrints ID: 441614
URI: http://eprints.soton.ac.uk/id/eprint/441614
ISSN: 0006-291X
PURE UUID: 29eec7ee-6367-430a-8079-d6ab06bda0e9
ORCID for Richard Oreffo: ORCID iD orcid.org/0000-0001-5995-6726

Catalogue record

Date deposited: 19 Jun 2020 16:33
Last modified: 09 Jan 2022 05:17

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Contributors

Author: C. Moura
Author: A Miranda
Author: Richard Oreffo ORCID iD
Author: Pieter De Beule

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