Ephemeral detection of Bonamia exitiosa (Haplosporida) in adult and larval European flat oysters Ostrea edulis in the Solent, United Kingdom
Ephemeral detection of Bonamia exitiosa (Haplosporida) in adult and larval European flat oysters Ostrea edulis in the Solent, United Kingdom
The haplosporidian parasite Bonamia exitiosa was detected using PCR in four adult and six larval brood samples of the European flat oyster Ostrea edulis from the Solent, UK. This represents the second reported detection of this parasite along the south coast of England. Adult oysters were collected and preserved from seabed populations or restoration broodstock cages between 2015 and 2018. The larvae within brooding adults sampled during 2017 and 2018 were also preserved. Molecular analysis of all samples was performed in 2019. The DNA of B. exitiosa was confirmed to be present within the gill tissue of one oyster within the Portsmouth wild fishery seabed population (n = 48), sampled in November 2015; the congeneric parasite Bonamia ostreae was not detected in this individual. This is the earliest record of B. exitiosa in the Solent. Concurrent presence of both B. ostreae and B. exitiosa, determined by DNA presence, was confirmed in the gill and heart tissue of three mature individuals from broodstock cages sampled in October 2017 (n = 99), two from a location on the River Hamble and one from the Camber Dock in Portsmouth Harbour. B. exitiosa was not detected in the November 2018 broodstock populations. A total of six larval broods were positive for B. exitiosa, with five also positive for B. ostreae. None of the brooding adults were positive for B. exitiosa suggesting that horizontal transmission from the surrounding environment to the brooding larvae is occurring. Further sampling of broodstock populations conducted by the Fish Health Inspectorate at the Centre for Environment, Fisheries and Aquaculture Science in June 2019 did not detect infection of O. edulis by B. exitiosa. These findings together suggest that the pathogen has not currently established in the area.
Bonamia exitiosa, Bonamia ostreae, Haplosporida, Molecular diagnosis, Ostrea edulis, Oyster
1-8
Helmer, Luke
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Hauton, Chris
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Bean, Tim
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Bass, David
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Hendy, Ian
b46e8a38-281d-4459-b9fd-f33efd8b4b22
Harris-scott, Eric
b02be754-9e66-4d0a-9b8c-f65ab0e56f84
Preston, Joanne
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1 July 2020
Helmer, Luke
87a6dbed-2d4c-4444-b39f-24090ab9754a
Hauton, Chris
7706f6ba-4497-42b2-8c6d-00df81676331
Bean, Tim
a24a11dd-fdc9-47a7-880c-7c990c3c867f
Bass, David
7058c349-be39-4a68-8c41-04efabdc8029
Hendy, Ian
b46e8a38-281d-4459-b9fd-f33efd8b4b22
Harris-scott, Eric
b02be754-9e66-4d0a-9b8c-f65ab0e56f84
Preston, Joanne
3e709180-12dc-4311-91e8-6ff5c87e96aa
Helmer, Luke, Hauton, Chris, Bean, Tim, Bass, David, Hendy, Ian, Harris-scott, Eric and Preston, Joanne
(2020)
Ephemeral detection of Bonamia exitiosa (Haplosporida) in adult and larval European flat oysters Ostrea edulis in the Solent, United Kingdom.
Journal of Invertebrate Pathology, 174, , [107421].
(doi:10.1016/j.jip.2020.107421).
Abstract
The haplosporidian parasite Bonamia exitiosa was detected using PCR in four adult and six larval brood samples of the European flat oyster Ostrea edulis from the Solent, UK. This represents the second reported detection of this parasite along the south coast of England. Adult oysters were collected and preserved from seabed populations or restoration broodstock cages between 2015 and 2018. The larvae within brooding adults sampled during 2017 and 2018 were also preserved. Molecular analysis of all samples was performed in 2019. The DNA of B. exitiosa was confirmed to be present within the gill tissue of one oyster within the Portsmouth wild fishery seabed population (n = 48), sampled in November 2015; the congeneric parasite Bonamia ostreae was not detected in this individual. This is the earliest record of B. exitiosa in the Solent. Concurrent presence of both B. ostreae and B. exitiosa, determined by DNA presence, was confirmed in the gill and heart tissue of three mature individuals from broodstock cages sampled in October 2017 (n = 99), two from a location on the River Hamble and one from the Camber Dock in Portsmouth Harbour. B. exitiosa was not detected in the November 2018 broodstock populations. A total of six larval broods were positive for B. exitiosa, with five also positive for B. ostreae. None of the brooding adults were positive for B. exitiosa suggesting that horizontal transmission from the surrounding environment to the brooding larvae is occurring. Further sampling of broodstock populations conducted by the Fish Health Inspectorate at the Centre for Environment, Fisheries and Aquaculture Science in June 2019 did not detect infection of O. edulis by B. exitiosa. These findings together suggest that the pathogen has not currently established in the area.
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Accepted/In Press date: 3 June 2020
e-pub ahead of print date: 6 June 2020
Published date: 1 July 2020
Keywords:
Bonamia exitiosa, Bonamia ostreae, Haplosporida, Molecular diagnosis, Ostrea edulis, Oyster
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Local EPrints ID: 442245
URI: http://eprints.soton.ac.uk/id/eprint/442245
ISSN: 0022-2011
PURE UUID: 726b40fb-85b6-4ca7-a900-ed0a312350e1
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Date deposited: 09 Jul 2020 16:39
Last modified: 17 Mar 2024 05:43
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Author:
Luke Helmer
Author:
Tim Bean
Author:
David Bass
Author:
Ian Hendy
Author:
Eric Harris-scott
Author:
Joanne Preston
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