Nanoclay-polyamine composite hydrogel for topical delivery of nitric oxide gas via innate gelation characteristics of laponite
Nanoclay-polyamine composite hydrogel for topical delivery of nitric oxide gas via innate gelation characteristics of laponite
Because nitric oxide (NO) gas is an endogenously produced signaling molecule related to numerous physiological functions, manystudies have been conducted to develop NO delivery systems for potential biomedical applications. However, NO is a reactive radical gas molecule that has a very short life-time and readily transforms into nitrogen oxide species via reaction with oxygen species. Therefore, it is necessary to develop an NO delivery carrier that allows local release of the NO gas at the site of application. In this study, Laponite (LP) nanoclay was used to fabricate an NO delivery carrier through the formation of Laponite–polyamine (LP–PAn) composites. The Laponite clay and pentaethylenehexamine (PEHA) formed a macromolecular structure by electrostatic interaction and the nitric oxide donor, N-diazeniumdiolate (NONOates), was synthesized into the LP–PAn composite. We investigated the conformation of the LP–PAn composite structure and the NO donor formation by ζ potential, X-ray diffraction, and UV–vis and Fourier transform infrared (FT-IR) spectroscopies and also by analyzing the NO release profile. Additionally, we confirmed the applicability in biomedical applications via a cell viability and in vitro endothelial cell tube formation assay.
2096-2103
Park, Kyungtae
49dc0f89-3793-476a-8c6b-e0dc7fe4ca03
Dawson, Jonathan
b220fe76-498d-47be-9995-92da6c289cf3
Oreffo, Richard
ff9fff72-6855-4d0f-bfb2-311d0e8f3778
Kim, Yanghee
de0d641b-c2cb-4e73-9ae2-e20d33689f5d
Hong, Jinkee
1a7277e4-0e22-4aab-adc5-ff05adc57289
8 June 2020
Park, Kyungtae
49dc0f89-3793-476a-8c6b-e0dc7fe4ca03
Dawson, Jonathan
b220fe76-498d-47be-9995-92da6c289cf3
Oreffo, Richard
ff9fff72-6855-4d0f-bfb2-311d0e8f3778
Kim, Yanghee
de0d641b-c2cb-4e73-9ae2-e20d33689f5d
Hong, Jinkee
1a7277e4-0e22-4aab-adc5-ff05adc57289
Park, Kyungtae, Dawson, Jonathan, Oreffo, Richard, Kim, Yanghee and Hong, Jinkee
(2020)
Nanoclay-polyamine composite hydrogel for topical delivery of nitric oxide gas via innate gelation characteristics of laponite.
Biomacromolecules, 21 (6), .
(doi:10.1021/acs.biomac.0c00086).
Abstract
Because nitric oxide (NO) gas is an endogenously produced signaling molecule related to numerous physiological functions, manystudies have been conducted to develop NO delivery systems for potential biomedical applications. However, NO is a reactive radical gas molecule that has a very short life-time and readily transforms into nitrogen oxide species via reaction with oxygen species. Therefore, it is necessary to develop an NO delivery carrier that allows local release of the NO gas at the site of application. In this study, Laponite (LP) nanoclay was used to fabricate an NO delivery carrier through the formation of Laponite–polyamine (LP–PAn) composites. The Laponite clay and pentaethylenehexamine (PEHA) formed a macromolecular structure by electrostatic interaction and the nitric oxide donor, N-diazeniumdiolate (NONOates), was synthesized into the LP–PAn composite. We investigated the conformation of the LP–PAn composite structure and the NO donor formation by ζ potential, X-ray diffraction, and UV–vis and Fourier transform infrared (FT-IR) spectroscopies and also by analyzing the NO release profile. Additionally, we confirmed the applicability in biomedical applications via a cell viability and in vitro endothelial cell tube formation assay.
Text
(2nd rebuttal) Biomacromolecules_manuscript_Final
- Accepted Manuscript
More information
Accepted/In Press date: 8 April 2020
e-pub ahead of print date: 8 April 2020
Published date: 8 June 2020
Additional Information:
Funding Information:
This work was supported by the Korea Health Technology R&D Project through the Korea Health Industry Development Institute (KHIDI), funded by the Ministry of Health & Welfare, Republic of Korea (HI18C2021) and Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science and ICT (NRF-2017R1E1A1A01074343). This work was supported by the UK-Korea Partnering Award jointly funded by the UK Medical Research Council (MRC) and the Korea Health Industry Development Institute (KHIDI) (grant number MC_PC_18015). J.I.D. gratefully acknowledges fellowship funding from the EPSRC (grant number EP/L010259/1).
Publisher Copyright:
Copyright © 2020 American Chemical Society.
Identifiers
Local EPrints ID: 442286
URI: http://eprints.soton.ac.uk/id/eprint/442286
ISSN: 1525-7797
PURE UUID: 33e69777-194b-4136-87ff-87e820d6dd4b
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Date deposited: 10 Jul 2020 16:41
Last modified: 17 Mar 2024 05:43
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Author:
Kyungtae Park
Author:
Jinkee Hong
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