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Nanopatterned titanium implants accelerate bone formation in vivo

Nanopatterned titanium implants accelerate bone formation in vivo
Nanopatterned titanium implants accelerate bone formation in vivo
Accelerated de novo formation of bone is a highly desirable aim of implants targeting musculoskeletal injuries. To date this has primarily been addressed by biologic factors. However, there is an unmet need for robust, highly reproducible yet economic alternative strategies that strongly induce osteogenic cell response. Here we present a surface engineering method of translating bioactive nanopatterns from polymeric in vitro studies to clinically relevant material for orthopedics: three-dimensional, large area metal. We use a titanium-based sol-gel whereby metal implants can be engineered to induce osteoinduction both in vitro and in vivo. We show that controlled disordered nanotopographies presented as pillars with 15-25 nm height and 100 nm diameter on titanium dioxide effectively induce osteogenesis when seeded with STRO-1 enriched human skeletal stem cells in vivo subcutaneous implantation in mice. After 28 days samples were retrieved, which showed 20-fold increase in osteogenic gene induction of nanopatterned substrates, indicating that the sol-gel nanopatterning method offers a promising route for translation to future clinical orthopedic implants.
coating, osteogenesis, prosthesis, sol-gel, stem cell
1944-8244
33541-33549
Oreffo, Richard
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Greer, Andrew
6979ae26-9faf-4a5b-9932-3621c5f3d075
Goriainov, Vitali
dc456832-0a5a-4816-8fb7-de132f044a99
Kanczler, Janos
eb8db9ff-a038-475f-9030-48eef2b0559c
Black, Cameron Russell Macgregor
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Turner, Lesley
9ce55e58-e149-4768-8618-388c3c9b7dae
Meek, R.
e24431ea-8c00-4ee0-8082-d17f0980bdf5
Burgess, Karl
b1cd8041-8abb-4025-bdba-985cd43bd217
MacLaren, Ian
e004e137-79f8-4190-be1b-76f9ae5fc6ba
Dalby, Matthew J.
25dcae6a-8289-4169-abb7-c45fff0bafdc
Gadegaard, Nikolaj
bf72c3d1-e6a7-48b4-a968-c496b655bd98
Oreffo, Richard
ff9fff72-6855-4d0f-bfb2-311d0e8f3778
Greer, Andrew
6979ae26-9faf-4a5b-9932-3621c5f3d075
Goriainov, Vitali
dc456832-0a5a-4816-8fb7-de132f044a99
Kanczler, Janos
eb8db9ff-a038-475f-9030-48eef2b0559c
Black, Cameron Russell Macgregor
7f616e37-4d80-4f60-ac8d-ba96e583db72
Turner, Lesley
9ce55e58-e149-4768-8618-388c3c9b7dae
Meek, R.
e24431ea-8c00-4ee0-8082-d17f0980bdf5
Burgess, Karl
b1cd8041-8abb-4025-bdba-985cd43bd217
MacLaren, Ian
e004e137-79f8-4190-be1b-76f9ae5fc6ba
Dalby, Matthew J.
25dcae6a-8289-4169-abb7-c45fff0bafdc
Gadegaard, Nikolaj
bf72c3d1-e6a7-48b4-a968-c496b655bd98

Oreffo, Richard, Greer, Andrew, Goriainov, Vitali, Kanczler, Janos, Black, Cameron Russell Macgregor, Turner, Lesley, Meek, R., Burgess, Karl, MacLaren, Ian, Dalby, Matthew J. and Gadegaard, Nikolaj (2020) Nanopatterned titanium implants accelerate bone formation in vivo. ACS Applied Materials & Interfaces, 12 (30), 33541-33549. (doi:10.1021/acsami.0c10273).

Record type: Article

Abstract

Accelerated de novo formation of bone is a highly desirable aim of implants targeting musculoskeletal injuries. To date this has primarily been addressed by biologic factors. However, there is an unmet need for robust, highly reproducible yet economic alternative strategies that strongly induce osteogenic cell response. Here we present a surface engineering method of translating bioactive nanopatterns from polymeric in vitro studies to clinically relevant material for orthopedics: three-dimensional, large area metal. We use a titanium-based sol-gel whereby metal implants can be engineered to induce osteoinduction both in vitro and in vivo. We show that controlled disordered nanotopographies presented as pillars with 15-25 nm height and 100 nm diameter on titanium dioxide effectively induce osteogenesis when seeded with STRO-1 enriched human skeletal stem cells in vivo subcutaneous implantation in mice. After 28 days samples were retrieved, which showed 20-fold increase in osteogenic gene induction of nanopatterned substrates, indicating that the sol-gel nanopatterning method offers a promising route for translation to future clinical orthopedic implants.

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Greer - nano Ti in vivo Author Accepted Manuscript - Accepted Manuscript
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More information

Accepted/In Press date: 6 July 2020
e-pub ahead of print date: 6 July 2020
Keywords: coating, osteogenesis, prosthesis, sol-gel, stem cell

Identifiers

Local EPrints ID: 443046
URI: http://eprints.soton.ac.uk/id/eprint/443046
ISSN: 1944-8244
PURE UUID: e5931441-35f7-4f9b-9ad2-3e343297052c
ORCID for Richard Oreffo: ORCID iD orcid.org/0000-0001-5995-6726
ORCID for Janos Kanczler: ORCID iD orcid.org/0000-0001-7249-0414

Catalogue record

Date deposited: 06 Aug 2020 16:36
Last modified: 26 Nov 2021 06:17

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Contributors

Author: Richard Oreffo ORCID iD
Author: Andrew Greer
Author: Vitali Goriainov
Author: Janos Kanczler ORCID iD
Author: Cameron Russell Macgregor Black
Author: Lesley Turner
Author: R. Meek
Author: Karl Burgess
Author: Ian MacLaren
Author: Matthew J. Dalby
Author: Nikolaj Gadegaard

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