The University of Southampton
University of Southampton Institutional Repository

Evolving applications of the egg: Chorioallantoic Membrane (CAM) assay and ex vivo organotypic culture of materials for bone tissue engineering

Evolving applications of the egg: Chorioallantoic Membrane (CAM) assay and ex vivo organotypic culture of materials for bone tissue engineering
Evolving applications of the egg: Chorioallantoic Membrane (CAM) assay and ex vivo organotypic culture of materials for bone tissue engineering
The chick chorioallantoic membrane (CAM) model has been around for over a century, applied in angiogenic, oncology, dental and xenograft research. Despite its often perceived archaic, redolent history, the CAM assay offers new and exciting opportunities for material and growth factor evaluation in bone tissue engineering. Currently, superior/improved experimental methodology for the CAM assay are difficult to identify, given an absence of scientific consensus in defining experimental approaches including, timing of inoculation with materials and the analysis of results. In addition, critically, regulatory and welfare issues impact upon experimental designs. Given such disparate points, this review details recent research using the ex vivo CAM assay and ex vivo organotypic culture to advance the field of bone tissue engineering and, highlights potential areas of improvement for their application based on recent developments within our group and the tissue engineering field.
2041-7314
Marshall, Karen
955e07ec-09e2-4464-aca7-65351afe19e3
Kanczler, Janos
eb8db9ff-a038-475f-9030-48eef2b0559c
Oreffo, Richard
ff9fff72-6855-4d0f-bfb2-311d0e8f3778
Marshall, Karen
955e07ec-09e2-4464-aca7-65351afe19e3
Kanczler, Janos
eb8db9ff-a038-475f-9030-48eef2b0559c
Oreffo, Richard
ff9fff72-6855-4d0f-bfb2-311d0e8f3778

Marshall, Karen, Kanczler, Janos and Oreffo, Richard (2020) Evolving applications of the egg: Chorioallantoic Membrane (CAM) assay and ex vivo organotypic culture of materials for bone tissue engineering. Journal of Tissue Engineering, 11 (204173142094273).

Record type: Article

Abstract

The chick chorioallantoic membrane (CAM) model has been around for over a century, applied in angiogenic, oncology, dental and xenograft research. Despite its often perceived archaic, redolent history, the CAM assay offers new and exciting opportunities for material and growth factor evaluation in bone tissue engineering. Currently, superior/improved experimental methodology for the CAM assay are difficult to identify, given an absence of scientific consensus in defining experimental approaches including, timing of inoculation with materials and the analysis of results. In addition, critically, regulatory and welfare issues impact upon experimental designs. Given such disparate points, this review details recent research using the ex vivo CAM assay and ex vivo organotypic culture to advance the field of bone tissue engineering and, highlights potential areas of improvement for their application based on recent developments within our group and the tissue engineering field.

Text
CAM review accepted version - Accepted Manuscript
Download (9MB)

More information

Accepted/In Press date: 26 June 2020
Published date: 20 October 2020

Identifiers

Local EPrints ID: 443060
URI: http://eprints.soton.ac.uk/id/eprint/443060
ISSN: 2041-7314
PURE UUID: d163d36c-1a67-4fab-866f-21f400d5ddfd
ORCID for Karen Marshall: ORCID iD orcid.org/0000-0002-6809-9807
ORCID for Janos Kanczler: ORCID iD orcid.org/0000-0001-7249-0414
ORCID for Richard Oreffo: ORCID iD orcid.org/0000-0001-5995-6726

Catalogue record

Date deposited: 07 Aug 2020 16:36
Last modified: 12 Jul 2024 04:03

Export record

Contributors

Author: Karen Marshall ORCID iD
Author: Janos Kanczler ORCID iD
Author: Richard Oreffo ORCID iD

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of http://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×