Evolving applications of the egg: Chorioallantoic Membrane (CAM) assay and ex vivo organotypic culture of materials for bone tissue engineering
Evolving applications of the egg: Chorioallantoic Membrane (CAM) assay and ex vivo organotypic culture of materials for bone tissue engineering
The chick chorioallantoic membrane (CAM) model has been around for over a century, applied in angiogenic, oncology, dental and xenograft research. Despite its often perceived archaic, redolent history, the CAM assay offers new and exciting opportunities for material and growth factor evaluation in bone tissue engineering. Currently, superior/improved experimental methodology for the CAM assay are difficult to identify, given an absence of scientific consensus in defining experimental approaches including, timing of inoculation with materials and the analysis of results. In addition, critically, regulatory and welfare issues impact upon experimental designs. Given such disparate points, this review details recent research using the ex vivo CAM assay and ex vivo organotypic culture to advance the field of bone tissue engineering and, highlights potential areas of improvement for their application based on recent developments within our group and the tissue engineering field.
Marshall, Karen
955e07ec-09e2-4464-aca7-65351afe19e3
Kanczler, Janos
eb8db9ff-a038-475f-9030-48eef2b0559c
Oreffo, Richard
ff9fff72-6855-4d0f-bfb2-311d0e8f3778
20 October 2020
Marshall, Karen
955e07ec-09e2-4464-aca7-65351afe19e3
Kanczler, Janos
eb8db9ff-a038-475f-9030-48eef2b0559c
Oreffo, Richard
ff9fff72-6855-4d0f-bfb2-311d0e8f3778
Marshall, Karen, Kanczler, Janos and Oreffo, Richard
(2020)
Evolving applications of the egg: Chorioallantoic Membrane (CAM) assay and ex vivo organotypic culture of materials for bone tissue engineering.
Journal of Tissue Engineering, 11 (204173142094273).
Abstract
The chick chorioallantoic membrane (CAM) model has been around for over a century, applied in angiogenic, oncology, dental and xenograft research. Despite its often perceived archaic, redolent history, the CAM assay offers new and exciting opportunities for material and growth factor evaluation in bone tissue engineering. Currently, superior/improved experimental methodology for the CAM assay are difficult to identify, given an absence of scientific consensus in defining experimental approaches including, timing of inoculation with materials and the analysis of results. In addition, critically, regulatory and welfare issues impact upon experimental designs. Given such disparate points, this review details recent research using the ex vivo CAM assay and ex vivo organotypic culture to advance the field of bone tissue engineering and, highlights potential areas of improvement for their application based on recent developments within our group and the tissue engineering field.
Text
CAM review accepted version
- Accepted Manuscript
More information
Accepted/In Press date: 26 June 2020
Published date: 20 October 2020
Identifiers
Local EPrints ID: 443060
URI: http://eprints.soton.ac.uk/id/eprint/443060
ISSN: 2041-7314
PURE UUID: d163d36c-1a67-4fab-866f-21f400d5ddfd
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Date deposited: 07 Aug 2020 16:36
Last modified: 17 Mar 2024 05:47
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Contributors
Author:
Karen Marshall
Author:
Janos Kanczler
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