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Evolving applications of the egg: Chorioallantoic Membrane (CAM) assay and ex vivo organotypic culture of materials for bone tissue engineering

Evolving applications of the egg: Chorioallantoic Membrane (CAM) assay and ex vivo organotypic culture of materials for bone tissue engineering
Evolving applications of the egg: Chorioallantoic Membrane (CAM) assay and ex vivo organotypic culture of materials for bone tissue engineering
The chick chorioallantoic membrane (CAM) model has been around for over a century, applied in angiogenic, oncology, dental and xenograft research. Despite its often perceived archaic, redolent history, the CAM assay offers new and exciting opportunities for material and growth factor evaluation in bone tissue engineering. Currently, superior/improved experimental methodology for the CAM assay are difficult to identify, given an absence of scientific consensus in defining experimental approaches including, timing of inoculation with materials and the analysis of results. In addition, critically, regulatory and welfare issues impact upon experimental designs. Given such disparate points, this review details recent research using the ex vivo CAM assay and ex vivo organotypic culture to advance the field of bone tissue engineering and, highlights potential areas of improvement for their application based on recent developments within our group and the tissue engineering field.
2041-7314
Oreffo, Richard
ff9fff72-6855-4d0f-bfb2-311d0e8f3778
Marshall, Karen
955e07ec-09e2-4464-aca7-65351afe19e3
Kanczler, Janos
eb8db9ff-a038-475f-9030-48eef2b0559c
Oreffo, Richard
ff9fff72-6855-4d0f-bfb2-311d0e8f3778
Marshall, Karen
955e07ec-09e2-4464-aca7-65351afe19e3
Kanczler, Janos
eb8db9ff-a038-475f-9030-48eef2b0559c

Oreffo, Richard, Marshall, Karen and Kanczler, Janos (2020) Evolving applications of the egg: Chorioallantoic Membrane (CAM) assay and ex vivo organotypic culture of materials for bone tissue engineering. Journal of Tissue Engineering. (In Press)

Record type: Article

Abstract

The chick chorioallantoic membrane (CAM) model has been around for over a century, applied in angiogenic, oncology, dental and xenograft research. Despite its often perceived archaic, redolent history, the CAM assay offers new and exciting opportunities for material and growth factor evaluation in bone tissue engineering. Currently, superior/improved experimental methodology for the CAM assay are difficult to identify, given an absence of scientific consensus in defining experimental approaches including, timing of inoculation with materials and the analysis of results. In addition, critically, regulatory and welfare issues impact upon experimental designs. Given such disparate points, this review details recent research using the ex vivo CAM assay and ex vivo organotypic culture to advance the field of bone tissue engineering and, highlights potential areas of improvement for their application based on recent developments within our group and the tissue engineering field.

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CAM review accepted version - Accepted Manuscript
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Accepted/In Press date: 26 June 2020

Identifiers

Local EPrints ID: 443060
URI: http://eprints.soton.ac.uk/id/eprint/443060
ISSN: 2041-7314
PURE UUID: d163d36c-1a67-4fab-866f-21f400d5ddfd
ORCID for Richard Oreffo: ORCID iD orcid.org/0000-0001-5995-6726
ORCID for Karen Marshall: ORCID iD orcid.org/0000-0002-6809-9807
ORCID for Janos Kanczler: ORCID iD orcid.org/0000-0001-7249-0414

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Date deposited: 07 Aug 2020 16:36
Last modified: 18 Feb 2021 17:39

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