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Use of an oxygen planar optode to assess the effect of high velocity microsprays on oxygen penetration in a human dental biofilms in-vitro

Use of an oxygen planar optode to assess the effect of high velocity microsprays on oxygen penetration in a human dental biofilms in-vitro
Use of an oxygen planar optode to assess the effect of high velocity microsprays on oxygen penetration in a human dental biofilms in-vitro
Background
Dental plaque biofilms are the causative agents of caries, gingivitis and periodontitis. Both mechanical and chemical strategies are used in routine oral hygiene strategies to reduce plaque build-up. If allowed to mature biofilms can create anoxic microenvironments leading to communities which harbor pathogenic Gram-negative anaerobes. When subjected to high velocity fluid jets and sprays biofilms can be fluidized which disrupts the biofilm structure and allows the more efficient delivery of antimicrobial agents.
Methods
To investigate how such jets may disrupt anoxic niches in the biofilm, we used planar optodes to measure the dissolved oxygen (DO) concentration at the base of in-vitro biofilms grown from human saliva and dental plaque. These biofilms were subject to “shooting” treatments with a commercial high velocity microspray (HVM) device.
Results
HVM treatment resulted in removal of much of the biofilm and a concurrent rapid shift from anoxic to oxic conditions at the base of the surrounding biofilm. We also assessed the impact of HVM treatment on the microbial community by tracking 7 target species by qPCR. There was a general reduction in copy numbers of the universal 16S RNA by approximately 95%, and changes of individual species in the target region ranged from approximately 1 to 4 log reductions.
Conclusion
We concluded that high velocity microsprays removed a sufficient amount of biofilm to disrupt the anoxic region at the biofilm-surface interface.
Mechanical disruption, biofilm, dissolved oxygen, microspray, oral, planar optodes
Khosravi, Yalda
e341065c-78aa-4780-9234-7e5f3cbe850f
Prasad Kandukuri, Raja Durga
c2366b19-f056-4350-a0e8-5be8453dcfa8
Palmer, Sara
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Gloag, Erin S.
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Borisov, Sergey M.
eceeb922-0fb8-4425-813e-71cd23601921
Starke, E.Michelle
52224939-da66-4070-afa6-01def56c4427
Ward, Marilyn
c0c450d1-882e-470f-b0d8-05b2e32b7fb1
Kumar, Purnima
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deBeer, Dirk
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Chennu, Arjun
756b7fe3-7981-4ac8-a577-0c996e456eff
Stoodley, Paul
08614665-92a9-4466-806e-20c6daeb483f
Khosravi, Yalda
e341065c-78aa-4780-9234-7e5f3cbe850f
Prasad Kandukuri, Raja Durga
c2366b19-f056-4350-a0e8-5be8453dcfa8
Palmer, Sara
05ad99fd-a12f-41e5-9b43-e6e0d22a55ca
Gloag, Erin S.
36de1738-c25d-4eb7-acd3-1ad55d9a7cee
Borisov, Sergey M.
eceeb922-0fb8-4425-813e-71cd23601921
Starke, E.Michelle
52224939-da66-4070-afa6-01def56c4427
Ward, Marilyn
c0c450d1-882e-470f-b0d8-05b2e32b7fb1
Kumar, Purnima
24a05e97-0489-4a30-a3eb-dac29ecc2d1c
deBeer, Dirk
6fcf8b7b-3f1e-470b-ac93-b2afa8b1f613
Chennu, Arjun
756b7fe3-7981-4ac8-a577-0c996e456eff
Stoodley, Paul
08614665-92a9-4466-806e-20c6daeb483f

Khosravi, Yalda, Prasad Kandukuri, Raja Durga, Palmer, Sara, Gloag, Erin S., Borisov, Sergey M., Starke, E.Michelle, Ward, Marilyn, Kumar, Purnima, deBeer, Dirk, Chennu, Arjun and Stoodley, Paul (2020) Use of an oxygen planar optode to assess the effect of high velocity microsprays on oxygen penetration in a human dental biofilms in-vitro. BMC Oral Health, 20 (1), [230]. (doi:10.1186/s12903-020-01217-0).

Record type: Article

Abstract

Background
Dental plaque biofilms are the causative agents of caries, gingivitis and periodontitis. Both mechanical and chemical strategies are used in routine oral hygiene strategies to reduce plaque build-up. If allowed to mature biofilms can create anoxic microenvironments leading to communities which harbor pathogenic Gram-negative anaerobes. When subjected to high velocity fluid jets and sprays biofilms can be fluidized which disrupts the biofilm structure and allows the more efficient delivery of antimicrobial agents.
Methods
To investigate how such jets may disrupt anoxic niches in the biofilm, we used planar optodes to measure the dissolved oxygen (DO) concentration at the base of in-vitro biofilms grown from human saliva and dental plaque. These biofilms were subject to “shooting” treatments with a commercial high velocity microspray (HVM) device.
Results
HVM treatment resulted in removal of much of the biofilm and a concurrent rapid shift from anoxic to oxic conditions at the base of the surrounding biofilm. We also assessed the impact of HVM treatment on the microbial community by tracking 7 target species by qPCR. There was a general reduction in copy numbers of the universal 16S RNA by approximately 95%, and changes of individual species in the target region ranged from approximately 1 to 4 log reductions.
Conclusion
We concluded that high velocity microsprays removed a sufficient amount of biofilm to disrupt the anoxic region at the biofilm-surface interface.

Text
Khosravi BMC Oral Health Accepted Version - Accepted Manuscript
Available under License Creative Commons Attribution.
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More information

Accepted/In Press date: 12 August 2020
e-pub ahead of print date: 21 August 2020
Keywords: Mechanical disruption, biofilm, dissolved oxygen, microspray, oral, planar optodes

Identifiers

Local EPrints ID: 443385
URI: http://eprints.soton.ac.uk/id/eprint/443385
PURE UUID: 0e3f1fa1-25d1-4edf-8e42-52a44ada0486
ORCID for Paul Stoodley: ORCID iD orcid.org/0000-0001-6069-273X

Catalogue record

Date deposited: 24 Aug 2020 16:31
Last modified: 28 Apr 2022 02:02

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Contributors

Author: Yalda Khosravi
Author: Raja Durga Prasad Kandukuri
Author: Sara Palmer
Author: Erin S. Gloag
Author: Sergey M. Borisov
Author: E.Michelle Starke
Author: Marilyn Ward
Author: Purnima Kumar
Author: Dirk deBeer
Author: Arjun Chennu
Author: Paul Stoodley ORCID iD

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