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Structured nanofilms comprising Laponite® and bone extracellular matrix for osteogenic differentiation of skeletal progenitor cells

Structured nanofilms comprising Laponite® and bone extracellular matrix for osteogenic differentiation of skeletal progenitor cells
Structured nanofilms comprising Laponite® and bone extracellular matrix for osteogenic differentiation of skeletal progenitor cells
Functionalized scaffolds hold promise for stem cell therapy by controlling stem cell fate and differentiation potential. Here, we have examined the potential of a 2-dimensional (2D) scaffold to stimulate bone regeneration. Solubilized extracellular matrix (ECM) from human bone tissue contains native extracellular cues for human skeletal cells that facilitate osteogenic differentiation. However, human bone ECM displays limited mechanical strength and degradation stability under physiological conditions, necessitating modification of the physical properties of ECM before it can be considered for tissue engineering applications. To increase the mechanical stability of ECM, we explored the potential of synthetic Laponite® (LAP) clay as a counter material to prepare a 2D scaffold using Layer-by-Layer (LbL) self-assembly. The LAP and ECM multilayer nanofilms (ECM/LAP film) were successfully generated through electrostatic and protein–clay interactions. Furthermore, to enhance the mechanical properties of the ECM/LAP film, application of a NaCl solution wash step, instead of deionized water following LAP deposition resulted in the generation of stable, multi-stacked LAP layers which displayed enhanced mechanical properties able to sustain human skeletal progenitor cell growth. The ECM/LAP films were not cytotoxic and, critically, showed enhanced osteogenic differentiation potential as a consequence of the synergistic effects of ECM and LAP. In summary, we demonstrate the fabrication of a novel ECM/LAP nanofilm layer material with potential application in hard tissue engineering.
Bone extracellular matrix, Laponite®, Layer-by-layer, Multilayer nanofilm, Osteogenic differentiation
0928-4931
Oreffo, Richard
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Choi, Daheui
25e093c2-d41f-4c8a-b3b7-ca3ff4f4ccde
Heo, Jiwoong
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Aviles Milan, Juan
de7da4ea-63e2-4737-9e42-c4e2c66b6a3e
Dawson, Jonathan
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Hong, Jinkee
2f392f3c-fae3-4fdf-9736-2a7eb6c1b10e
Kim, Yanghee
de0d641b-c2cb-4e73-9ae2-e20d33689f5d
Oreffo, Richard
ff9fff72-6855-4d0f-bfb2-311d0e8f3778
Choi, Daheui
25e093c2-d41f-4c8a-b3b7-ca3ff4f4ccde
Heo, Jiwoong
a9dbaa76-17d8-4262-8bc1-0d44a7dd7aae
Aviles Milan, Juan
de7da4ea-63e2-4737-9e42-c4e2c66b6a3e
Dawson, Jonathan
b220fe76-498d-47be-9995-92da6c289cf3
Hong, Jinkee
2f392f3c-fae3-4fdf-9736-2a7eb6c1b10e
Kim, Yanghee
de0d641b-c2cb-4e73-9ae2-e20d33689f5d

Oreffo, Richard, Choi, Daheui, Heo, Jiwoong, Aviles Milan, Juan, Dawson, Jonathan, Hong, Jinkee and Kim, Yanghee (2021) Structured nanofilms comprising Laponite® and bone extracellular matrix for osteogenic differentiation of skeletal progenitor cells. Materials Science and Engineering C, 118, [111440]. (doi:10.1016/j.msec.2020.111440).

Record type: Article

Abstract

Functionalized scaffolds hold promise for stem cell therapy by controlling stem cell fate and differentiation potential. Here, we have examined the potential of a 2-dimensional (2D) scaffold to stimulate bone regeneration. Solubilized extracellular matrix (ECM) from human bone tissue contains native extracellular cues for human skeletal cells that facilitate osteogenic differentiation. However, human bone ECM displays limited mechanical strength and degradation stability under physiological conditions, necessitating modification of the physical properties of ECM before it can be considered for tissue engineering applications. To increase the mechanical stability of ECM, we explored the potential of synthetic Laponite® (LAP) clay as a counter material to prepare a 2D scaffold using Layer-by-Layer (LbL) self-assembly. The LAP and ECM multilayer nanofilms (ECM/LAP film) were successfully generated through electrostatic and protein–clay interactions. Furthermore, to enhance the mechanical properties of the ECM/LAP film, application of a NaCl solution wash step, instead of deionized water following LAP deposition resulted in the generation of stable, multi-stacked LAP layers which displayed enhanced mechanical properties able to sustain human skeletal progenitor cell growth. The ECM/LAP films were not cytotoxic and, critically, showed enhanced osteogenic differentiation potential as a consequence of the synergistic effects of ECM and LAP. In summary, we demonstrate the fabrication of a novel ECM/LAP nanofilm layer material with potential application in hard tissue engineering.

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Accepted/In Press date: 22 August 2020
e-pub ahead of print date: 25 August 2020
Published date: January 2021
Additional Information: Funding Information: This work was supported by the UK-Korea Partnering Award funded by the UK Medical Research Council (MRC) and the Korea Health Industry Development Institute (KHIDI) (grant number MC_PC_18015 ), Jonathan Dawson's UK Engineering and Physical Sciences Research Council ( EPSRC) fellowship (grant number EP/L010259/1 ), and RO's UK Regenerative Medicine Platform Acellular / Smart Materials – 3D Architecture ( MR/R015651/1 ). And this research was also supported by a grant of the Korea Health Technology R&D Project through the Korea Health Industry Development Institute (KHIDI), funded by the Ministry of Health & Welfare , Republic of Korea ( HI18C2021 ), and Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science and ICT ( NRF-2017R1E1A1A01074343 ). Publisher Copyright: © 2020 Elsevier B.V.
Keywords: Bone extracellular matrix, Laponite®, Layer-by-layer, Multilayer nanofilm, Osteogenic differentiation

Identifiers

Local EPrints ID: 443572
URI: http://eprints.soton.ac.uk/id/eprint/443572
ISSN: 0928-4931
PURE UUID: 22204390-e08d-41e8-a7ce-222172cfca5d
ORCID for Richard Oreffo: ORCID iD orcid.org/0000-0001-5995-6726
ORCID for Jonathan Dawson: ORCID iD orcid.org/0000-0002-6712-0598
ORCID for Yanghee Kim: ORCID iD orcid.org/0000-0002-5312-3448

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Date deposited: 03 Sep 2020 00:38
Last modified: 06 Jun 2024 04:22

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Contributors

Author: Richard Oreffo ORCID iD
Author: Daheui Choi
Author: Jiwoong Heo
Author: Juan Aviles Milan
Author: Jonathan Dawson ORCID iD
Author: Jinkee Hong
Author: Yanghee Kim ORCID iD

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