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Structured nanofilms comprising Laponite® and bone extracellular matrix for osteogenic differentiation of skeletal progenitor cells

Structured nanofilms comprising Laponite® and bone extracellular matrix for osteogenic differentiation of skeletal progenitor cells
Structured nanofilms comprising Laponite® and bone extracellular matrix for osteogenic differentiation of skeletal progenitor cells
Functionalized scaffolds hold promise for stem cell therapy by controlling stem cell fate and differentiation potential. Here, we have examined the potential of a 2-dimensional (2D) scaffold to stimulate bone regeneration. Solubilized extracellular matrix (ECM) from human bone tissue contains native extracellular cues for human skeletal cells that facilitate osteogenic differentiation. However, human bone ECM displays limited mechanical strength and degradation stability under physiological conditions, necessitating modification of the physical properties of ECM before it can be considered for tissue engineering applications. To increase the mechanical stability of ECM, we explored the potential of synthetic Laponite® (LAP) clay as a counter material to prepare a 2D scaffold using Layer-by-Layer (LbL) self-assembly. The LAP and ECM multilayer nanofilms (ECM/LAP film) were successfully generated through electrostatic and protein–clay interactions. Furthermore, to enhance the mechanical properties of the ECM/LAP film, application of a NaCl solution wash step, instead of deionized water following LAP deposition resulted in the generation of stable, multi-stacked LAP layers which displayed enhanced mechanical properties able to sustain human skeletal progenitor cell growth. The ECM/LAP films were not cytotoxic and, critically, showed enhanced osteogenic differentiation potential as a consequence of the synergistic effects of ECM and LAP. In summary, we demonstrate the fabrication of a novel ECM/LAP nanofilm layer material with potential application in hard tissue engineering.
Bone extracellular matrix, Laponite®, Layer-by-layer, Multilayer nanofilm, Osteogenic differentiation
0928-4931
Oreffo, Richard
ff9fff72-6855-4d0f-bfb2-311d0e8f3778
Choi, Daheui
25e093c2-d41f-4c8a-b3b7-ca3ff4f4ccde
Heo, Jiwoong
a9dbaa76-17d8-4262-8bc1-0d44a7dd7aae
Aviles Milan, Juan
de7da4ea-63e2-4737-9e42-c4e2c66b6a3e
Dawson, Jonathan
b220fe76-498d-47be-9995-92da6c289cf3
Hong, Jinkee
2f392f3c-fae3-4fdf-9736-2a7eb6c1b10e
Kim, Yanghee
de0d641b-c2cb-4e73-9ae2-e20d33689f5d
Oreffo, Richard
ff9fff72-6855-4d0f-bfb2-311d0e8f3778
Choi, Daheui
25e093c2-d41f-4c8a-b3b7-ca3ff4f4ccde
Heo, Jiwoong
a9dbaa76-17d8-4262-8bc1-0d44a7dd7aae
Aviles Milan, Juan
de7da4ea-63e2-4737-9e42-c4e2c66b6a3e
Dawson, Jonathan
b220fe76-498d-47be-9995-92da6c289cf3
Hong, Jinkee
2f392f3c-fae3-4fdf-9736-2a7eb6c1b10e
Kim, Yanghee
de0d641b-c2cb-4e73-9ae2-e20d33689f5d

Oreffo, Richard, Choi, Daheui, Heo, Jiwoong, Aviles Milan, Juan, Dawson, Jonathan, Hong, Jinkee and Kim, Yanghee (2020) Structured nanofilms comprising Laponite® and bone extracellular matrix for osteogenic differentiation of skeletal progenitor cells. Materials Science and Engineering C, 118, [111440]. (doi:10.1016/j.msec.2020.111440).

Record type: Article

Abstract

Functionalized scaffolds hold promise for stem cell therapy by controlling stem cell fate and differentiation potential. Here, we have examined the potential of a 2-dimensional (2D) scaffold to stimulate bone regeneration. Solubilized extracellular matrix (ECM) from human bone tissue contains native extracellular cues for human skeletal cells that facilitate osteogenic differentiation. However, human bone ECM displays limited mechanical strength and degradation stability under physiological conditions, necessitating modification of the physical properties of ECM before it can be considered for tissue engineering applications. To increase the mechanical stability of ECM, we explored the potential of synthetic Laponite® (LAP) clay as a counter material to prepare a 2D scaffold using Layer-by-Layer (LbL) self-assembly. The LAP and ECM multilayer nanofilms (ECM/LAP film) were successfully generated through electrostatic and protein–clay interactions. Furthermore, to enhance the mechanical properties of the ECM/LAP film, application of a NaCl solution wash step, instead of deionized water following LAP deposition resulted in the generation of stable, multi-stacked LAP layers which displayed enhanced mechanical properties able to sustain human skeletal progenitor cell growth. The ECM/LAP films were not cytotoxic and, critically, showed enhanced osteogenic differentiation potential as a consequence of the synergistic effects of ECM and LAP. In summary, we demonstrate the fabrication of a novel ECM/LAP nanofilm layer material with potential application in hard tissue engineering.

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Manuscript_Revised_Final Accepted version - Accepted Manuscript
Restricted to Repository staff only until 25 August 2021.
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More information

Accepted/In Press date: 22 August 2020
e-pub ahead of print date: 25 August 2020
Keywords: Bone extracellular matrix, Laponite®, Layer-by-layer, Multilayer nanofilm, Osteogenic differentiation

Identifiers

Local EPrints ID: 443572
URI: http://eprints.soton.ac.uk/id/eprint/443572
ISSN: 0928-4931
PURE UUID: 22204390-e08d-41e8-a7ce-222172cfca5d
ORCID for Richard Oreffo: ORCID iD orcid.org/0000-0001-5995-6726
ORCID for Jonathan Dawson: ORCID iD orcid.org/0000-0002-6712-0598

Catalogue record

Date deposited: 03 Sep 2020 00:38
Last modified: 18 Feb 2021 17:11

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