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Semi-quantitative detection of inflammatory biomarkers using a laser-patterned multiplexed lateral flow device

Semi-quantitative detection of inflammatory biomarkers using a laser-patterned multiplexed lateral flow device
Semi-quantitative detection of inflammatory biomarkers using a laser-patterned multiplexed lateral flow device

Inflammatory markers including C-reactive protein (CRP) and procalcitonin (PCT) have been shown to be useful biomarkers to improve triage speed and prevent the inappropriate use of antibiotics for infections such as pneumonia. Here, we present a novel and exciting solution to guide the administration of antibiotic treatment via rapid, semi-quantitative and multiplexed detection of CRP and PCT using an advanced lateral flow device (LFD) designed to have multiple parallel flow-paths, produced via the precise laser-based partitioning of the single flow-path of a standard LFD. Each flow-path within this multiplexed LFD has a unique detection capability which permits tailored detection of CRP within a predefined cut-off range (20 μg/mL - 100 μg/mL) and PCT above a pre-defined threshold (0.5 ng/mL). We demonstrate the use of this LFD in the successful detection of CRP and PCT semi-quantitatively within spiked human serum samples. This multiplexed near-patient assay has potential for development into a rapid triage and treatment of patients with suspected pneumonia.

C-reactive protein, Lateral-flow device, Multiplexed, Point-of-care, Procalcitonin, Semi-quantitative
0039-9140
Iles, Alice H.
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He, Peijun J.W.
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Katis, Ioannis N.
f92dfb8f-610d-4877-83f6-fd26a571df12
Galanis, Panagiotis P.
4457b788-deef-4293-ab39-76f501b9529d
John, Anto J.U.K.
518d3739-712a-4681-9d0c-3e4fccf00454
Elkington, Paul
60828c7c-3d32-47c9-9fcc-6c4c54c35a15
Eason, Robert W.
e38684c3-d18c-41b9-a4aa-def67283b020
Sones, Collin L.
9de9d8ee-d394-46a5-80b7-e341c0eed0a8
Iles, Alice H.
4c7e0b28-8a6e-442e-88ca-a06e6e1a453d
He, Peijun J.W.
2e303166-6aa5-4a09-b22e-440d96a54a9f
Katis, Ioannis N.
f92dfb8f-610d-4877-83f6-fd26a571df12
Galanis, Panagiotis P.
4457b788-deef-4293-ab39-76f501b9529d
John, Anto J.U.K.
518d3739-712a-4681-9d0c-3e4fccf00454
Elkington, Paul
60828c7c-3d32-47c9-9fcc-6c4c54c35a15
Eason, Robert W.
e38684c3-d18c-41b9-a4aa-def67283b020
Sones, Collin L.
9de9d8ee-d394-46a5-80b7-e341c0eed0a8

Iles, Alice H., He, Peijun J.W., Katis, Ioannis N., Galanis, Panagiotis P., John, Anto J.U.K., Elkington, Paul, Eason, Robert W. and Sones, Collin L. (2022) Semi-quantitative detection of inflammatory biomarkers using a laser-patterned multiplexed lateral flow device. Talanta, 237, [122944]. (doi:10.1016/j.talanta.2021.122944).

Record type: Article

Abstract

Inflammatory markers including C-reactive protein (CRP) and procalcitonin (PCT) have been shown to be useful biomarkers to improve triage speed and prevent the inappropriate use of antibiotics for infections such as pneumonia. Here, we present a novel and exciting solution to guide the administration of antibiotic treatment via rapid, semi-quantitative and multiplexed detection of CRP and PCT using an advanced lateral flow device (LFD) designed to have multiple parallel flow-paths, produced via the precise laser-based partitioning of the single flow-path of a standard LFD. Each flow-path within this multiplexed LFD has a unique detection capability which permits tailored detection of CRP within a predefined cut-off range (20 μg/mL - 100 μg/mL) and PCT above a pre-defined threshold (0.5 ng/mL). We demonstrate the use of this LFD in the successful detection of CRP and PCT semi-quantitatively within spiked human serum samples. This multiplexed near-patient assay has potential for development into a rapid triage and treatment of patients with suspected pneumonia.

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In preparation date: 2020
Accepted/In Press date: 7 October 2021
Published date: 15 January 2022
Additional Information: Funding Information: The authors acknowledge the funding received via the Engineering and Physical Sciences Research Council (EPSRC) Grant Nos. EP/N004388/1 , EP/P025757/1 and EP/M027260/1 . The underpinning RDM data for this paper can be found at https://doi.org/10.5258/SOTON/D1560 . Publisher Copyright: © 2021 Elsevier B.V.
Keywords: C-reactive protein, Lateral-flow device, Multiplexed, Point-of-care, Procalcitonin, Semi-quantitative

Identifiers

Local EPrints ID: 444037
URI: http://eprints.soton.ac.uk/id/eprint/444037
ISSN: 0039-9140
PURE UUID: 3f64ee34-3604-4ade-8a24-b8dce0518ac2
ORCID for Ioannis N. Katis: ORCID iD orcid.org/0000-0002-2016-557X
ORCID for Panagiotis P. Galanis: ORCID iD orcid.org/0000-0002-2028-5803
ORCID for Paul Elkington: ORCID iD orcid.org/0000-0003-0390-0613
ORCID for Robert W. Eason: ORCID iD orcid.org/0000-0001-9704-2204

Catalogue record

Date deposited: 23 Sep 2020 16:30
Last modified: 09 Jan 2022 03:59

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Contributors

Author: Alice H. Iles
Author: Peijun J.W. He
Author: Panagiotis P. Galanis ORCID iD
Author: Paul Elkington ORCID iD
Author: Robert W. Eason ORCID iD
Author: Collin L. Sones

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