In vitro bioassay-guided identification of anticancer properties from Moringa oleifera Lam. Leaf against MDA-MB-231 cell line
In vitro bioassay-guided identification of anticancer properties from Moringa oleifera Lam. Leaf against MDA-MB-231 cell line
Moringa oleifera Lam. (MO) is a medicinal plant distributed across the Middle East, Asia, and Africa. MO has been used in the traditional treatment of various diseases including cancer. This study aimed to perform bioassay-guided fractionation and identification of bioactive compounds from MO leaf against MDA-MB-231 breast cancer cells. MO leaf was sequentially extracted with hexane, ethyl acetate (EtOAc), and ethanol. The most effective extract was subjected to fractionation. MO extract and its derived fractions were continuously screened for anti-cancer activities. The strongest fraction was selected for re-fractionation and identification of bioactive compounds using LC-ESI-QTOF-MS/MS analysis. The best anticancer activities were related to the fraction no. 7-derived crude EtOAc extract. This fraction significantly reduced cell viability and clonogenic growth and increased cells apoptosis. Moreover, sub-fraction no. 7.7-derived fraction no. 7 was selected for the identification of bioactive compounds. There were 10 candidate compounds tentatively identified by LC-ESI-QTOF-MS. Three of identified compounds (7-octenoic acid, oleamide, and 1-phenyl-2-pentanol) showed anticancer activities by inducing cell cycle arrest and triggering apoptosis through suppressed Bcl-2 expression which subsequently promotes activation of caspase 3, indicators for the apoptosis pathway. This study identified 10 candidate compounds that may have potential in the field of anticancer substances.
1-phenyl-2-pentanol, 7-octenoic acid, LC-ESI-QTOF-MS/MS, MDA-MB-231, Moringa oleifera, Oleamide, Triple negative breast cancer
1-20
Wisitpongpun, Prapakorn
dedbdfc4-92d7-44b6-890f-3fede7cdd83c
Suphrom, Nungruthai
790be07b-e1ae-4a91-bfe5-c3acafaad2d1
Potup, Pachuen
27c48f4e-5f52-44fd-ae76-d77060ea5b8b
Nuengchamnong, Nitra
27d0d844-2d7d-4db4-a471-826bbd25f4a0
Calder, Philip
1797e54f-378e-4dcb-80a4-3e30018f07a6
Usuwanthim, Kanchana
da5a4117-5ca6-4fff-b07d-66eb42575df4
December 2020
Wisitpongpun, Prapakorn
dedbdfc4-92d7-44b6-890f-3fede7cdd83c
Suphrom, Nungruthai
790be07b-e1ae-4a91-bfe5-c3acafaad2d1
Potup, Pachuen
27c48f4e-5f52-44fd-ae76-d77060ea5b8b
Nuengchamnong, Nitra
27d0d844-2d7d-4db4-a471-826bbd25f4a0
Calder, Philip
1797e54f-378e-4dcb-80a4-3e30018f07a6
Usuwanthim, Kanchana
da5a4117-5ca6-4fff-b07d-66eb42575df4
Wisitpongpun, Prapakorn, Suphrom, Nungruthai, Potup, Pachuen, Nuengchamnong, Nitra, Calder, Philip and Usuwanthim, Kanchana
(2020)
In vitro bioassay-guided identification of anticancer properties from Moringa oleifera Lam. Leaf against MDA-MB-231 cell line.
Pharmaceuticals, 13 (12), , [464].
(doi:10.3390/ph13120464).
Abstract
Moringa oleifera Lam. (MO) is a medicinal plant distributed across the Middle East, Asia, and Africa. MO has been used in the traditional treatment of various diseases including cancer. This study aimed to perform bioassay-guided fractionation and identification of bioactive compounds from MO leaf against MDA-MB-231 breast cancer cells. MO leaf was sequentially extracted with hexane, ethyl acetate (EtOAc), and ethanol. The most effective extract was subjected to fractionation. MO extract and its derived fractions were continuously screened for anti-cancer activities. The strongest fraction was selected for re-fractionation and identification of bioactive compounds using LC-ESI-QTOF-MS/MS analysis. The best anticancer activities were related to the fraction no. 7-derived crude EtOAc extract. This fraction significantly reduced cell viability and clonogenic growth and increased cells apoptosis. Moreover, sub-fraction no. 7.7-derived fraction no. 7 was selected for the identification of bioactive compounds. There were 10 candidate compounds tentatively identified by LC-ESI-QTOF-MS. Three of identified compounds (7-octenoic acid, oleamide, and 1-phenyl-2-pentanol) showed anticancer activities by inducing cell cycle arrest and triggering apoptosis through suppressed Bcl-2 expression which subsequently promotes activation of caspase 3, indicators for the apoptosis pathway. This study identified 10 candidate compounds that may have potential in the field of anticancer substances.
Text
pharmaceuticals
- Accepted Manuscript
More information
Accepted/In Press date: 4 December 2020
e-pub ahead of print date: 15 December 2020
Published date: December 2020
Additional Information:
Funding Information:
This research was funded by the Royal Golden Jubilee Ph.D. Program (grant no. PHD/0002/2559), Thailand Science Research and Innovation, and Naresuan University (grant no. R2564B002).
Publisher Copyright:
© 2020 by the authors. Licensee MDPI, Basel, Switzerland.
Keywords:
1-phenyl-2-pentanol, 7-octenoic acid, LC-ESI-QTOF-MS/MS, MDA-MB-231, Moringa oleifera, Oleamide, Triple negative breast cancer
Identifiers
Local EPrints ID: 445595
URI: http://eprints.soton.ac.uk/id/eprint/445595
ISSN: 1424-8247
PURE UUID: 392e58b4-9a61-4d87-bbdd-1a9971b174b6
Catalogue record
Date deposited: 16 Dec 2020 18:14
Last modified: 17 Mar 2024 02:42
Export record
Altmetrics
Contributors
Author:
Prapakorn Wisitpongpun
Author:
Nungruthai Suphrom
Author:
Pachuen Potup
Author:
Nitra Nuengchamnong
Author:
Kanchana Usuwanthim
Download statistics
Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.
View more statistics
