The University of Southampton
University of Southampton Institutional Repository
Warning ePrints Soton is experiencing an issue with some file downloads not being available. We are working hard to fix this. Please bear with us.

Paternal bisphenol A exposure induces testis and sperm pathologies in mice offspring: possibly due to oxidative stress?

Paternal bisphenol A exposure induces testis and sperm pathologies in mice offspring: possibly due to oxidative stress?
Paternal bisphenol A exposure induces testis and sperm pathologies in mice offspring: possibly due to oxidative stress?

Bisphenol A (BPA), an endocrine and metabolic disruptor, is widely used to manufacture polycarbonate plastics and epoxy resins. Accumulating evidence suggests that paternal BPA exposure adversely affects male germlines and results in atypical reproductive phenotypes that might persist for generations to come. Our study investigated this exposure on testicular architecture and sperm quality in mouse offspring, and characterised underlying molecular mechanism(s). A total of 18 immature male Swiss albino mice (3.5 weeks old) were randomly divided into three groups and treated as follows: Group I, no treatment (sham control); Group II, sterile corn oil only (vehicle control); Group III, BPA (400 μg/kg) in sterile corn oil. At 9.5 weeks old, F0 males were mated with unexposed females. F0 offspring (F1 generation) were monitored for postnatal development for 10 weeks. At 11.5 weeks old, the animals were sacrificed to examine testicular architecture, sperm parameters, including DNA integrity, and oxidative stress biomarkers. Results showed that BPA significantly induced changes in the body and testis weights of the F0 and F1 generation BPA lineages compared to F0 and F1 generation control lineages. A decrease in sperm count and motility with further, increased sperm abnormalities, no or few sperm DNA alterations and elevated levels of MDA, PC and NO were recorded. Similar effects were found in BPA exposed F0 males, but were more pronounced in the F0 offspring. In addition, BPA caused alterations in the testicular architecture. These pathological changes extended transgenerationally to F1 generation males’ mice, but the pathological changes were more pronounced in the F1 generation. Our findings demonstrate that the biological and health BPA impacts do not end in paternal adults, but are passed on to offspring generations. Hence, linking observed testis and sperm abnormalities in the F1 generation to BPA exposure of their parental line was evident in this work. The findings also illustrate that oxidative stress appears to be a molecular component of the testis and sperm pathologies.

BPA, Endocrine disruptors, Multigenerational, Oxidative stress, Sperm quality, Testis architecture, Transgenerational
948-955
Al-Griw, Mohamed A.
7a5e0326-8d98-4f08-ad27-0f24a0227d41
Alghazeer, Rabia O.
5acc4581-0ada-46b8-a611-abe68d254b92
Salama, Naser M.
daa9b6e1-44cf-4217-83f1-32f6bc67f207
Lwaleed, Bashir
e7c59131-82ad-4a14-a227-7370e91e3f21
Eskandrani, Areej A.
9ea29608-3bf3-4635-ba85-5da49a7d18d6
Alansari, Wafa S.
fbffc044-da74-42ae-8306-be91e78a3aae
Alnajeebi, Afnan M.
49bfc26b-5cee-47f8-ad64-8fbb3464ff07
Babteen, Nouf A.
4bfe386d-b798-4b89-9f77-211239279bc4
Elnfati, Abdul Hakim
3073068e-2810-494e-ae93-6e06d4e64955
Al-Griw, Mohamed A.
7a5e0326-8d98-4f08-ad27-0f24a0227d41
Alghazeer, Rabia O.
5acc4581-0ada-46b8-a611-abe68d254b92
Salama, Naser M.
daa9b6e1-44cf-4217-83f1-32f6bc67f207
Lwaleed, Bashir
e7c59131-82ad-4a14-a227-7370e91e3f21
Eskandrani, Areej A.
9ea29608-3bf3-4635-ba85-5da49a7d18d6
Alansari, Wafa S.
fbffc044-da74-42ae-8306-be91e78a3aae
Alnajeebi, Afnan M.
49bfc26b-5cee-47f8-ad64-8fbb3464ff07
Babteen, Nouf A.
4bfe386d-b798-4b89-9f77-211239279bc4
Elnfati, Abdul Hakim
3073068e-2810-494e-ae93-6e06d4e64955

Al-Griw, Mohamed A., Alghazeer, Rabia O., Salama, Naser M., Lwaleed, Bashir, Eskandrani, Areej A., Alansari, Wafa S., Alnajeebi, Afnan M., Babteen, Nouf A. and Elnfati, Abdul Hakim (2021) Paternal bisphenol A exposure induces testis and sperm pathologies in mice offspring: possibly due to oxidative stress? Saudi Journal of Biological Sciences, 28 (1), 948-955. (doi:10.1016/j.sjbs.2020.11.003).

Record type: Article

Abstract

Bisphenol A (BPA), an endocrine and metabolic disruptor, is widely used to manufacture polycarbonate plastics and epoxy resins. Accumulating evidence suggests that paternal BPA exposure adversely affects male germlines and results in atypical reproductive phenotypes that might persist for generations to come. Our study investigated this exposure on testicular architecture and sperm quality in mouse offspring, and characterised underlying molecular mechanism(s). A total of 18 immature male Swiss albino mice (3.5 weeks old) were randomly divided into three groups and treated as follows: Group I, no treatment (sham control); Group II, sterile corn oil only (vehicle control); Group III, BPA (400 μg/kg) in sterile corn oil. At 9.5 weeks old, F0 males were mated with unexposed females. F0 offspring (F1 generation) were monitored for postnatal development for 10 weeks. At 11.5 weeks old, the animals were sacrificed to examine testicular architecture, sperm parameters, including DNA integrity, and oxidative stress biomarkers. Results showed that BPA significantly induced changes in the body and testis weights of the F0 and F1 generation BPA lineages compared to F0 and F1 generation control lineages. A decrease in sperm count and motility with further, increased sperm abnormalities, no or few sperm DNA alterations and elevated levels of MDA, PC and NO were recorded. Similar effects were found in BPA exposed F0 males, but were more pronounced in the F0 offspring. In addition, BPA caused alterations in the testicular architecture. These pathological changes extended transgenerationally to F1 generation males’ mice, but the pathological changes were more pronounced in the F1 generation. Our findings demonstrate that the biological and health BPA impacts do not end in paternal adults, but are passed on to offspring generations. Hence, linking observed testis and sperm abnormalities in the F1 generation to BPA exposure of their parental line was evident in this work. The findings also illustrate that oxidative stress appears to be a molecular component of the testis and sperm pathologies.

Text
Paternal bisphenol A exposure induces testis and sperm pathologies in mice offspring - Accepted Manuscript
Download (8MB)
Text
Paternal bisphenol - Version of Record
Download (1MB)

More information

Accepted/In Press date: 1 November 2020
e-pub ahead of print date: 11 November 2020
Published date: January 2021
Additional Information: © 2020 The Authors.
Keywords: BPA, Endocrine disruptors, Multigenerational, Oxidative stress, Sperm quality, Testis architecture, Transgenerational

Identifiers

Local EPrints ID: 445600
URI: http://eprints.soton.ac.uk/id/eprint/445600
PURE UUID: eea51b97-2596-442a-8705-374f7b44bc0a

Catalogue record

Date deposited: 17 Dec 2020 17:31
Last modified: 25 Nov 2021 17:45

Export record

Altmetrics

Contributors

Author: Mohamed A. Al-Griw
Author: Rabia O. Alghazeer
Author: Naser M. Salama
Author: Bashir Lwaleed
Author: Areej A. Eskandrani
Author: Wafa S. Alansari
Author: Afnan M. Alnajeebi
Author: Nouf A. Babteen
Author: Abdul Hakim Elnfati

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of http://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×