Hypoxic regulation of preimplantation embryos: lessons from human embryonic stem cells
Hypoxic regulation of preimplantation embryos: lessons from human embryonic stem cells
Development of the preimplantation embryo is reliant on nutrients present in the milieu of the reproductive tract. While carbohydrates, amino acids, lipids, and micronutrients are often considered when discussing preimplantation embryo nutrition, environmental oxygen is frequently overlooked. Although oxygen is not classically considered a nutrient, it is an important component of the in vitro culture environment and a critical regulator of cellular physiology. Oxygen is required to sustain an oxidative metabolism but when oxygen becomes limited, cells mount a physiological response driven by a family of transcription factors termed 'hypoxia inducible factors' which promote expression of a multitude of oxygen sensitive genes. It is this hypoxic response that is responsible not only for the switch to a glycolytic metabolism but also for a plethora of other cellular responses. There has been much debate in recent years over which environmental oxygen tension is preferential for the culture of preimplantation embryos. The review will evaluate this question and highlights how research using human embryonic stem cells can inform our understanding of why culturing under physiological oxygen tensions may be beneficial for the development of embryos generated through clinical in vitro fertilisation.
F41–F51
Houghton, Franchesca D.
53946041-127e-45a8-9edb-bf4b3c23005f
1 January 2021
Houghton, Franchesca D.
53946041-127e-45a8-9edb-bf4b3c23005f
Houghton, Franchesca D.
(2021)
Hypoxic regulation of preimplantation embryos: lessons from human embryonic stem cells.
Reproduction, 161 (1), .
(doi:10.1530/REP-20-0322).
Abstract
Development of the preimplantation embryo is reliant on nutrients present in the milieu of the reproductive tract. While carbohydrates, amino acids, lipids, and micronutrients are often considered when discussing preimplantation embryo nutrition, environmental oxygen is frequently overlooked. Although oxygen is not classically considered a nutrient, it is an important component of the in vitro culture environment and a critical regulator of cellular physiology. Oxygen is required to sustain an oxidative metabolism but when oxygen becomes limited, cells mount a physiological response driven by a family of transcription factors termed 'hypoxia inducible factors' which promote expression of a multitude of oxygen sensitive genes. It is this hypoxic response that is responsible not only for the switch to a glycolytic metabolism but also for a plethora of other cellular responses. There has been much debate in recent years over which environmental oxygen tension is preferential for the culture of preimplantation embryos. The review will evaluate this question and highlights how research using human embryonic stem cells can inform our understanding of why culturing under physiological oxygen tensions may be beneficial for the development of embryos generated through clinical in vitro fertilisation.
Text
Accepted manuscript
- Accepted Manuscript
Text
Accepted Figures
- Accepted Manuscript
Text
Accepted Table
- Accepted Manuscript
More information
Accepted/In Press date: 24 November 2020
e-pub ahead of print date: 1 January 2021
Published date: 1 January 2021
Identifiers
Local EPrints ID: 446182
URI: http://eprints.soton.ac.uk/id/eprint/446182
ISSN: 0022-4251
PURE UUID: 42c57823-e4b0-4295-b9cb-2a7bc4a70968
Catalogue record
Date deposited: 26 Jan 2021 17:32
Last modified: 06 Jun 2024 01:43
Export record
Altmetrics
Download statistics
Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.
View more statistics
