3D cyclorama for digital unrolling and visualisation of deformed tubes
3D cyclorama for digital unrolling and visualisation of deformed tubes
Colonic crypts are tubular glands that multiply through a symmetric branching process called crypt fission. During the early stages of colorectal cancer, the normal fission process is disturbed, leading to asymmetrical branching or budding. The challenging shapes of the budding crypts make it difficult to prepare paraffin sections for conventional histology, resulting in colonic cross sections with crypts that are only partially visible. To study crypt budding in situ and in three dimensions (3D), we employ X-ray micro-computed tomography to image intact colons, and a new method we developed (3D cyclorama) to digitally unroll them. Here, we present, verify and validate our ‘3D cyclorama’ method that digitally unrolls deformed tubes of non-uniform thickness. It employs principles from electrostatics to reform the tube into a series of onion-like surfaces, which are mapped onto planar panoramic views. This enables the study of features extending over several layers of the tube’s depth, demonstrated here by two case studies: (i) microvilli in the human placenta and (ii) 3D-printed adhesive films for drug delivery. Our 3D cyclorama method can provide novel insights into a wide spectrum of applications where digital unrolling or flattening is necessary, including long bones, teeth roots and ancient scrolls.
Rossides, Harry
8ea65ff8-3ba0-46d3-9f88-18553aedfb7d
Pender, Sylvia
62528b03-ec42-41bb-80fe-48454c2c5242
Schneider, Philipp
a810f925-4808-44e4-8a4a-a51586f9d7ad
19 July 2021
Rossides, Harry
8ea65ff8-3ba0-46d3-9f88-18553aedfb7d
Pender, Sylvia
62528b03-ec42-41bb-80fe-48454c2c5242
Schneider, Philipp
a810f925-4808-44e4-8a4a-a51586f9d7ad
Rossides, Harry, Pender, Sylvia and Schneider, Philipp
(2021)
3D cyclorama for digital unrolling and visualisation of deformed tubes.
Scientific Reports, 11 (1), [14672].
(doi:10.1038/s41598-021-93184-x).
Abstract
Colonic crypts are tubular glands that multiply through a symmetric branching process called crypt fission. During the early stages of colorectal cancer, the normal fission process is disturbed, leading to asymmetrical branching or budding. The challenging shapes of the budding crypts make it difficult to prepare paraffin sections for conventional histology, resulting in colonic cross sections with crypts that are only partially visible. To study crypt budding in situ and in three dimensions (3D), we employ X-ray micro-computed tomography to image intact colons, and a new method we developed (3D cyclorama) to digitally unroll them. Here, we present, verify and validate our ‘3D cyclorama’ method that digitally unrolls deformed tubes of non-uniform thickness. It employs principles from electrostatics to reform the tube into a series of onion-like surfaces, which are mapped onto planar panoramic views. This enables the study of features extending over several layers of the tube’s depth, demonstrated here by two case studies: (i) microvilli in the human placenta and (ii) 3D-printed adhesive films for drug delivery. Our 3D cyclorama method can provide novel insights into a wide spectrum of applications where digital unrolling or flattening is necessary, including long bones, teeth roots and ancient scrolls.
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In preparation date: 2021
Accepted/In Press date: 15 June 2021
Published date: 19 July 2021
Additional Information:
Funding Information:
This study was supported by the Engineering and Physical Sciences Research Council (EPSRC) Doctoral Training Partnership (DTP) (EP/N509747/1), the Institute for Life Sciences, University of Southampton, Southampton, UK, and Nikon X-Tek Systems Ltd., Tring, UK.
Funding Information:
This study was supported by the Engineering and Physical Sciences Research Council (EPSRC) Doctoral Training Partnership (DTP) (EP/N509747/1), UK, the Institute for Life Sciences, University of Southampton, Southampton, UK and Nikon X-Tek Systems Ltd., Tring, UK. The authors would like to thank Diamond Light Source for granting beamtime, and the staff of beamline I13-2, Andrew J. Bodey, Shashidhara Marathe, and Kazimir Wanelik for assistance with testing, data collection, and data processing. Special thanks go to Rohan M. Lewis and Eleni Palaiologou for providing the serial block-face scanning electron microscopy (SBF SEM) stack of the placental tissue, and to Dimitrios G. Fatouros, Georgios K. Eleftheriadis, and Orestis L. Katsamenis for sharing the μCT stack of the pharmaceutical film. Thanks goes also to the staff at the μ-VIS X-ray Imaging Centre, the Histochemistry Research Unit, the Biomedical Imaging Unit, and the Biomedical Research Facility at the University of Southampton, Southampton, UK, for their assistance and access to their facilities. The authors also thank David S. Chatelet for the thorough testing of the 3D cyclorama Fiji plugin.
Publisher Copyright:
© 2021, The Author(s).
Identifiers
Local EPrints ID: 448073
URI: http://eprints.soton.ac.uk/id/eprint/448073
ISSN: 2045-2322
PURE UUID: 43c63908-3dfa-4368-8b60-dd7f2be5c413
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Date deposited: 01 Apr 2021 15:41
Last modified: 17 Mar 2024 06:28
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