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3D cyclorama for digital unrolling and visualisation of deformed tubes

3D cyclorama for digital unrolling and visualisation of deformed tubes
3D cyclorama for digital unrolling and visualisation of deformed tubes
Colonic crypts are tubular glands that multiply through a symmetric branching process called crypt fission. During the early stages of colorectal cancer, the normal fission process is disturbed, leading to asymmetrical branching or budding. The challenging shapes of the budding crypts make it difficult to prepare paraffin sections for conventional histology, resulting in colonic cross sections with crypts that are only partially visible. To study crypt budding in situ and in three dimensions (3D), we employ X-ray micro-computed tomography to image intact colons, and a new method we developed (3D cyclorama) to digitally unroll them. Here, we present, verify and validate our ‘3D cyclorama’ method that digitally unrolls deformed tubes of non-uniform thickness. It employs principles from electrostatics to reform the tube into a series of onion-like surfaces, which are mapped onto planar panoramic views. This enables the study of features extending over several layers of the tube’s depth, demonstrated here by two case studies: (i) microvilli in the human placenta and (ii) 3D-printed adhesive films for drug delivery. Our 3D cyclorama method can provide novel insights into a wide spectrum of applications where digital unrolling or flattening is necessary, including long bones, teeth roots and ancient scrolls.
2045-2322
Rossides, Harry
8ea65ff8-3ba0-46d3-9f88-18553aedfb7d
Pender, Sylvia
62528b03-ec42-41bb-80fe-48454c2c5242
Schneider, Philipp
a810f925-4808-44e4-8a4a-a51586f9d7ad
Rossides, Harry
8ea65ff8-3ba0-46d3-9f88-18553aedfb7d
Pender, Sylvia
62528b03-ec42-41bb-80fe-48454c2c5242
Schneider, Philipp
a810f925-4808-44e4-8a4a-a51586f9d7ad

Rossides, Harry, Pender, Sylvia and Schneider, Philipp (2021) 3D cyclorama for digital unrolling and visualisation of deformed tubes. Scientific Reports, 11 (1), [14672]. (doi:10.1038/s41598-021-93184-x).

Record type: Article

Abstract

Colonic crypts are tubular glands that multiply through a symmetric branching process called crypt fission. During the early stages of colorectal cancer, the normal fission process is disturbed, leading to asymmetrical branching or budding. The challenging shapes of the budding crypts make it difficult to prepare paraffin sections for conventional histology, resulting in colonic cross sections with crypts that are only partially visible. To study crypt budding in situ and in three dimensions (3D), we employ X-ray micro-computed tomography to image intact colons, and a new method we developed (3D cyclorama) to digitally unroll them. Here, we present, verify and validate our ‘3D cyclorama’ method that digitally unrolls deformed tubes of non-uniform thickness. It employs principles from electrostatics to reform the tube into a series of onion-like surfaces, which are mapped onto planar panoramic views. This enables the study of features extending over several layers of the tube’s depth, demonstrated here by two case studies: (i) microvilli in the human placenta and (ii) 3D-printed adhesive films for drug delivery. Our 3D cyclorama method can provide novel insights into a wide spectrum of applications where digital unrolling or flattening is necessary, including long bones, teeth roots and ancient scrolls.

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Author accepted manuscript - Accepted Manuscript
Restricted to Repository staff only until 14 June 2022.
Available under License Creative Commons Attribution.
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Text
Author accepted supplementary - Accepted Manuscript
Restricted to Repository staff only until 14 June 2022.
Available under License Creative Commons Attribution.
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More information

In preparation date: 2021
Accepted/In Press date: 15 June 2021
Published date: 19 July 2021

Identifiers

Local EPrints ID: 448073
URI: http://eprints.soton.ac.uk/id/eprint/448073
ISSN: 2045-2322
PURE UUID: 43c63908-3dfa-4368-8b60-dd7f2be5c413
ORCID for Harry Rossides: ORCID iD orcid.org/0000-0002-7547-0256
ORCID for Sylvia Pender: ORCID iD orcid.org/0000-0001-6332-0333
ORCID for Philipp Schneider: ORCID iD orcid.org/0000-0001-7499-3576

Catalogue record

Date deposited: 01 Apr 2021 15:41
Last modified: 26 Nov 2021 03:10

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