Nuclear hyperpolarization of (1-13C)-pyruvate in aqueous solution by proton-relayed side-Arm hydrogenation
Nuclear hyperpolarization of (1-13C)-pyruvate in aqueous solution by proton-relayed side-Arm hydrogenation
We employ Parahydrogen Induced Polarization with Side-Arm Hydrogenation (PHIP-SAH) to polarize (1-13C)-pyruvate. We introduce a new method called proton-relayed side-Arm hydrogenation (PR-SAH) in which an intermediate proton is used to transfer polarization from the side-Arm to the 13C-labelled site of the pyruvate before hydrolysis. This significantly reduces the cost and effort needed to prepare the precursor for radio-frequency transfer experiments while still maintaining acceptable polarization transfer efficiency. Experimentally we have attained on average 4.33% 13C polarization in an aqueous solution of (1-13C)-pyruvate after about 10 seconds of cleavage and extraction. PR-SAH is a promising pulsed NMR method for hyperpolarizing 13C-labelled metabolites in solution, conducted entirely in high magnetic field.
1772-1778
Dagys, Laurynas
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Jagtap, Anil P.
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Korchak, Sergey
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Mamone, Salvatore
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Saul, Philip
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Levitt, Malcolm H.
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Glöggler, Stefan
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7 March 2021
Dagys, Laurynas
0de61597-b152-4bee-a934-123a9d2de883
Jagtap, Anil P.
298f2515-ada9-43d7-96e7-a8f3d6d3a1e3
Korchak, Sergey
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Mamone, Salvatore
7f4c620f-8bb6-4eea-a95f-1173ce69bf80
Saul, Philip
7efad9b8-3e7a-4385-a1f3-ca489df0674c
Levitt, Malcolm H.
bcc5a80a-e5c5-4e0e-9a9a-249d036747c3
Glöggler, Stefan
ac98f078-6073-4783-973f-281616bdf3e6
Dagys, Laurynas, Jagtap, Anil P., Korchak, Sergey, Mamone, Salvatore, Saul, Philip, Levitt, Malcolm H. and Glöggler, Stefan
(2021)
Nuclear hyperpolarization of (1-13C)-pyruvate in aqueous solution by proton-relayed side-Arm hydrogenation.
Analyst, 146 (5), .
(doi:10.1039/d0an02389b).
Abstract
We employ Parahydrogen Induced Polarization with Side-Arm Hydrogenation (PHIP-SAH) to polarize (1-13C)-pyruvate. We introduce a new method called proton-relayed side-Arm hydrogenation (PR-SAH) in which an intermediate proton is used to transfer polarization from the side-Arm to the 13C-labelled site of the pyruvate before hydrolysis. This significantly reduces the cost and effort needed to prepare the precursor for radio-frequency transfer experiments while still maintaining acceptable polarization transfer efficiency. Experimentally we have attained on average 4.33% 13C polarization in an aqueous solution of (1-13C)-pyruvate after about 10 seconds of cleavage and extraction. PR-SAH is a promising pulsed NMR method for hyperpolarizing 13C-labelled metabolites in solution, conducted entirely in high magnetic field.
Text
d0an02389b
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Accepted/In Press date: 15 January 2021
e-pub ahead of print date: 15 January 2021
Published date: 7 March 2021
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Local EPrints ID: 448389
URI: http://eprints.soton.ac.uk/id/eprint/448389
ISSN: 0003-2654
PURE UUID: 90e43c1e-7e88-4d5a-a814-39a250fe1e1d
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Date deposited: 21 Apr 2021 16:32
Last modified: 17 Mar 2024 02:52
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Author:
Laurynas Dagys
Author:
Anil P. Jagtap
Author:
Sergey Korchak
Author:
Salvatore Mamone
Author:
Philip Saul
Author:
Stefan Glöggler
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